View More View Less
  • 1 University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
  • 2 University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Over the last few years several porcine epidemic diarrhoea (PED) outbreaks have been discovered in Europe including the first PED case in Slovenia in January 2015. The aim of this study was to determine when PED virus (PEDV) infection started in Slovenia. Serum samples collected between 2012 and 2016 were tested. Three hundred and seventy-five serum samples were collected from 132 Slovenian small, one-site pig farms. Samples were tested for PEDV antibodies utilising three different serological methods: commercially-available indirect ELISA, in-house blocking ELISA test and Immunoperoxidase Monolayer Assay (IPMA) test. One hundred and seventy (45.33%) tested samples were found positive by the commercially-available ELISA test kit, and 10 (5.68%) of these 170 samples found positive were positive by the in-house blocking ELISA. Only these 10 samples were collected from a farm where clinical signs of PED infection had been observed and PEDV was confirmed by RT-PCR methodology; the other 160 samples were collected randomly. Thirty-two samples with the highest S/P value obtained with the commercial ELISA were all negative with IPMA. Reasons for the high variance in the results obtained remain unclear; more research is required to ensure higher sensitivity and specificity in terms of PEDV antibody tests and other PED diagnostic methods.

  • Biovet Porcine Epidemic Diarrhea Virus antibody test kit, ELISA (2015): http://biovet.ca/data/documents/prod-doc/PEDV%20ELISA%20kit%20EN.pdf. Accessed 14 March 2017.

  • Bøtner, A., Nielsen, J. and Bille-Hansen, V. (1994): Isolation of porcine reproductive and respiratory syndrome (PRRS) virus in a Danish swine herd and experimental infection of pregnant gilts with the virus. Vet. Microbiol. 40, 351360.

    • Search Google Scholar
    • Export Citation
  • Bøtner, A., Strandbygaard, B., van der Poel, W., Hakze-van der Honing, R., Steinbach, F., Dastjerdi, A., Grasland, B., Bigault, L., Widén, F. and Svedlund, G. (2015): Interlaboratory comparison of assays for detection of antibodies against Porcine Epidemic Diarrhea virus. Proc. ESVV, Montpellier, France. p. 236.

    • Search Google Scholar
    • Export Citation
  • Carvajal, A., Lanza, I., Diego, R., Rubio, P. and Cámenes, P. (1995): Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhea virus and its antibodies. J. Vet. Diagn. Invest. 7, 6064.

    • Search Google Scholar
    • Export Citation
  • Gerber, P. F., Gong, Q., Huang, Y. W., Wang, C., Holtkamp, D. and Opriessnig, T. (2014): Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA. Vet. J. 202, 3336.

    • Search Google Scholar
    • Export Citation
  • Jung, K. and Saif, L. J. (2015): Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet. J. 204, 134143.

    • Search Google Scholar
    • Export Citation
  • Leidenberger, S., Schröder, C., Zani, L., Auste, A., Pinette, M., Ambagala, A., Nikolin, V., de Smit, H., Beer, M. and Blome, S. (2017): Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies. Sci. Rep. 7, 10825. http://doi.org/10.1038/s41598-017-11160-w.

    • Search Google Scholar
    • Export Citation
  • Nagy, B., Nagy, G., Meder, M. and Mocsári, E. (1996): Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary. Acta Vet. Hung. 44, 919.

    • Search Google Scholar
    • Export Citation
  • OIE (2014): Infection with porcine epidemic diarrhoea virus. http://www.oie.int/fileadmin/Home/eng/Our_scientific_expertise/docs/pdf/A_factsheet_PEDV.pdf.

    • Search Google Scholar
    • Export Citation
  • Pensaert, M. B. and de Bouck, P. (1978): A new coronavirus-like particle associated with diarrhea in swine. Arch. Virol. 58, 243247.

  • Pijpers, A., van Nieuwstadt, A. P., Terpstra, C. and Verheijden, J. H. (1993): Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs. Vet. Rec. 132, 129131.

    • Search Google Scholar
    • Export Citation
  • Pritchard, G. C., Paton, D. J., Wibberley, G. and Ibata, G. (1999): Transmissible gastroenteritis and porcine epidemic diarrhoea in Britain. Vet. Rec. 144, 616618.

    • Search Google Scholar
    • Export Citation
  • Puranaveja, S., Poolperm, P., Lertwatcharasarakul, P., Kesdaengsakonwut, S., Boonsoongnern, A., Urairong, K., Kitikoon, P., Choojai, P., Kedkovid, R., Teankum, K. and Thanawongnuwech, R. (2009): Chinese-like strain of porcine epidemic diarrhea virus, Thailand. Emerg. Infect. Dis. 15, 11121115.

    • Search Google Scholar
    • Export Citation
  • Saif, L. J., Pensaert, M. B., Sestak, K., Yeo, S. G. and Jung, K. (2012): Coronaviruses. In: Zimmerman, J. J., Karriker, L. A., Ramirez, A., Schwartz, K. J. and Stevenson, G. W. (eds) Diseases of Swine. 10th edition. Wiley-Blackwell, Ames, Iowa. pp. 501524.

    • Search Google Scholar
    • Export Citation
  • Shibata, I., Tsuda, T., Mori, M., Ono, M., Sueyoshi, M. and Uruno, K. (2000): Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet. Microbiol. 72, 173182.

    • Search Google Scholar
    • Export Citation
  • Song, D. and Park, B. (2012): Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes 44, 167175.

    • Search Google Scholar
    • Export Citation
  • Sørensen, K. J., Bøtner, A., Madsen, E. S., Strandbygaard, B. and Nielsen, J. (1997): Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus. Vet. Microbiol. 56, 18.

    • Search Google Scholar
    • Export Citation
  • Stevenson, G. W., Hoang, H., Schwartz, K. J., Burrough, E. R., Sun, D., Madson, D., Cooper, V. L., Pillatzki, A., Gauger, P., Schmitt, B. J., Koster, L. G., Killian, M. L. and Yoon, K. J. (2013): Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J. Vet. Diagn. Invest. 25, 649654.

    • Search Google Scholar
    • Export Citation
  • Toplak, I., Štukelj, M., Rihtarič, D., Hostnik, P. and Grom, J. (2015): First detection of porcine epidemic diarrhea virus in Slovenia, 2015. Proc. ESVV, Montpellier, France. p. 178.

    • Search Google Scholar
    • Export Citation
  • Wood, E. N. (1997): An apparently new syndrome of porcine epidemic diarrhoea. Vet. Rec. 100, 243244.

The author instruction is available in PDF.
Please, download the file from HERE.

 

MANUSCRIPT SUBMISSION

  • Impact Factor (2019): 0.991
  • Scimago Journal Rank (2019): 0.372
  • SJR Hirsch-Index (2019): 32
  • SJR Quartile Score (2019): Q2 Veterinary (miscellaneous)
  • Impact Factor (2018): 1.059
  • Scimago Journal Rank (2018): 0.517
  • SJR Hirsch-Index (2018): 30
  • SJR Quartile Score (2018): Q2 Veterinary (miscellaneous)

Language: English

Founded in 1951
Publication: One volume of four issues annually
Publication Programme: 2020. Vol. 68.
Indexing and Abstracting Services:

  • Biological Abstracts

     

  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Subscribers can access the electronic version of every printed article.

Senior editors

Editor(s)-in-Chief: Benkő, Mária

Managing Editor(s): Székely, András

Editorial Board

      Dénes, Béla
      Eszterbauer, Edit
      Fébel, Hedvig
      Fodor, László
      Harrach, Balázs
      Andras Komaromy (USA)
      Peter Massanyi (Slovak Republic)
      Nagy, Béla
      Németh, Tibor
      Neogrády, Zsuzsanna
      Kurt Pfister (Germany)
      Solti, László
      Szabó, József
      Vajdovich, Péter
      Varga, János
      Štefan, Vilĉek (Slovak Republic)
      Vörös, Károly
      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793