The authors studied the present status of Hungarian indigenous sheep breeds based on the genetic background of scrapie resistance. The aim of this investigation was to estimate the relative frequency of prion haplotypes, genotypes and risk categories, as well as to reveal the efficiency of the scrapie eradication programme achieved over the last decade. A novel approach in the characterisation of prion by using its genic variation was also implemented. The authors established that the proportion of deleterious sites (%) can be a useful indicator of the eradication programme. Based on a large sample size, it was confirmed that the scrapie resistance of the Cikta breed is low, and the classification of this breed according to risk category has not improved. However, the frequent genotype ARQ and risk category 3 can also be considered characteristic of the breed. The careful use of these genotypes is permitted and will contribute to the maintenance of breed diversity. The response of prion genic variation to selection for scrapie resistance in the other breeds (Tsigai, Milking Tsigai, White Racka, Black Racka and Gyimes Racka) was definitely successful.
Agrobiogen GmBH (2016): Scrapie Resistenz. Agrobiogen GmbH Biotechnologie. URL: http://www.agrobiogen.de. Accessed 25 March 2016.
Áldásy, P. and Süveges, T. (1964): The incidence of scrapie in Hungary [in Hungarian]. Magy. Allatorvosok 19, 463–465.
Álvarez, I., Gutiérrez, J. P., Royo, L. J., Fernández, I. and Goyache, F. (2009): Quantifying diversity losses due to selection for scrapie resistance in three endangered Spanish sheep breeds using microsatellite information. Prev. Vet. Med. 91, 172–178.
Álvarez, I., Royo, L. J., Gutiérrez, J. P., Fernández, I., Arranz, J. J. and Goyache, F. (2007): Genetic diversity loss due to selection for scrapie resistance in the rare Spanish Xalda sheep breed. Livest. Sci. 111, 204–212.
Baylis, M., Chihota, C., Stevenson, E., Goldmann, W., Smith, A., Sivam, K., Tongue, S. and Gravenor, M. B. (2004): Risk of scrapie in British sheep of different prion protein genotype. J. Gen. Virol. 85, 2735–2470.
Baylis, M., Goldmann, W., Houston, F., Cairns, D., Chong, A., Ross, A., Smith, A., Hunter, N. and McLean, A. R. (2002): Scrapie epidemic in a fully PrP-genotyped sheep flock. J. Gen. Virol. 83, 2907–2914.
Bodó, I. (ed.) (2000): Living Heritage – Old Historical Hungarian Livestock. Agroinform Publishing and Printing Ltd., Budapest. 125 pp.
Bostedt, H. and Dedié, K. (1996): Infectious diseases of the whole organism. Viral diseases: scrapie [in German]. In: Bostedt, H. and Dedié, K (eds) Schaf-und Ziegenkrankheiten. Verlag Eugen Ulmer, Stuttgart. pp. 73–75.
Cameron, C., Bell-Rogers, P., McDowall, R., Rebelo, A. R. and Cai, H. Y. (2014): Prion protein genotypes of sheep as determined from 3343 samples submitted from Ontario and other provinces of Canada from 2005 to 2012. Can. J. Vet. Res. 78, 260–266.
Dell Inc. (2015): Dell Statistica (data analysis software system), version 13. http://www.software. dell.com.
Dexler, H. (1931): Scrapie [in German]. In: Stang, V. and Wirth, D. (eds) Tierheilkunde und Tierzucht. Eine Enzyklopädie der praktischen Nutztierkunde. Urban & Schwarzenberg, Berlin–Wien. 807 pp.
Drögemüller, C., Leeb, T. and Distl, O. (2001): PrP genotype frequencies in German breeding sheep and the potential to breed for resistance to scrapie. Vet. Rec. 149, 349–352.
European Commission (2001): Regulation (EC) No 999/2001 of the European Parliament and of the Council of 22 May 2001, laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies.
Fésüs, L., Zsolnai, A., Horogh, G. and Anton, I. (2004): Scrapie of sheep 2. Prion genotype frequencies in Hungarian indigenous populations [in Hungarian, with English abstract]. Magy. Allatorvosok 126, 670–675.
Fésüs, L., Zsolnai, A., Anton, I. and Sáfár, L. (2008): Breeding for scrapie resistance in the Hungarian sheep population. Acta Vet. Hung. 56, 173–180.
Foster, J. and Hunter, N. (1998): Transmissible spongiform encephalopathies: transmission, mechanism of disease, and persistence. Curr. Opin. Microbiol. 1, 442–447.
FVM (2004): Decree 22/2004 (II. 27.) FVM of the Ministry of Agriculture and Rural Development on modification of FVM Decree 69/2003. (VI. 25.) Prevention, control and eradication of transmissible spongiform encephalopathies [in Hungarian]. Magyar Közlöny [Hungarian Gazette] 21, 1836.
Gáspárdy, A., Kukovics, S., Anton, I., Zsolnai, A. and Komlósi, I. (2014): Comprehensive examination of the biochemical and DNA polymorphisms of Hungarian Tsigai varieties [in Hungarian]. Állatteny. Takarm. 63, 123–135.
Government of Hungary (2017): Report B/17568 on food chain security and the allocation of the food chain management fee [in Hungarian]. Presented by Dr. Sándor Fazekas, Minister of Agriculture, Budapest, September 2017, http://www.parlament.hu/irom40/17568/17568.pdf
Ipate, I., Strasser, C., Ionita, L., Strateanu, A., Strasser, H. and Enache, L. M. (2013): Risk of scrapie for Romanian Turcana Sheep of prion protein genotype ARQ/ARQ. Rom. Biotech. Lett. 18, 8245–8252.
Kang, H. E., Mo, Y., Abd Rahim, R., Lee, H. M. and Ryou, C. (2017): Prion diagnosis: Application of real-time quaking-induced conversion. Review article. Biomed. Res. Int. Article ID 5413936, 8 pages, to be found at <https://www.hindawi.com/journals/bmri/2017/5413936/> (quoted 07.11.2017)
Kim, Y.-C. and Jeong, B.-H. (2017): The first report of prion-related protein gene (PRNT) polymorphisms in goat. Acta Vet. Hung. 65, 291–300.
Kluge, J. P. and Glávits, R. (1993): Hungary remains free of scrapie and bovine spongiform encephalopathy (BSE). Acta Vet. Hung. 41, 325–328.
McCutcheon, S., Hunter, N. and Houston, F. (2005): Use of a new immunoassay to measure PrPSc levels in scrapie-infected sheep brains reveals PrP genotype-specific differences. J. Immunol. Methods 298, 119–128.
Nagy, B., Anton, I., Sáfár, L., Fésüs, L. and Zsolnai, A. (2009): Association between PrP genotypes and selected growth traits of Hungarian Merino and German Mutton Merino rams. Arch. Tierz. 52, 613–617.
Prusiner, S. B. (1982): Novel proteinaceous infectious particles cause scrapie. Science 216, 136–144.
Rabenau, H. F. (2009): Part III. Special microbiological diagnostics [in German], 2 Prionen: 28 Diagnostik prionbedingter Erkrankungen, TSE-Erreger, Übertragungswege bei Tieren. In: Neumeister, B., Geiss, H. K., Braun, R. W. and Kimmig, P. (eds) Mikrobiologische Diagnostik – Bakteriologie – Mykologie – Virologie – Parasitologie. Georg Thieme Verlag, Stuttgart. pp. 635–639.
Stepanek, O. and Horin, P. (2017): Genetic diversity of the prion protein gene (PRNP) coding sequence in Czech sheep and evaluation of the national breeding programme for resistance to scrapie in the Czech Republic. J. Appl. Genet. 58, 111–121.
Viorca, C., Vlaic, A., Padeanu, I., Daraban, S., Voia, S., Catoi, C., Constantinescu, R. and Vicovan, G. (2008): The primer extension technique for the polymorphism detection at ovine PRN-P locus. Scientific Papers: Animal Science and Biotechnologies 41, 40–44.