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  • 1 University of Veterinary Medicine Budapest, H-2225 Üllő-Dóra major, Hungary
  • 2 MTA-SZIE Large Animal Clinical Research Group, Üllő-Dóra major, Hungary
  • 3 Szent István University, Gödöllő, Hungary
Open access

The aim of this study was to examine the effect of twin pregnancy, fetal laterality, the number of corpora lutea (CL) and cavitary CL on pregnancy losses in Holstein-Friesian cows with a positive pregnancy diagnosis based on ultrasonography between days 29–42 after AI. Pregnancy was confirmed by transrectal palpation between days 57–70 after AI and at the time of drying-off as well. Twin pregnancy rate was 8.4% at the time of the early pregnancy examination. Pregnancy loss did not differ between singleton- and twin-carrying animals either between days 57–70 of gestation or at drying-off. More losses occurred in singletons between days 29–42 and 57–70 in cows with cavitary than in cows with noncavitary CL (12.1% vs. 3.6%; P < 0.05) and in cows with double CL than in cows with single CL (7.3% vs. 3.6% %; P < 0.05). Between days 57–70 of gestation and drying-off this difference was still significant (20.7% vs. 3.7%; P < 0.001), while it was non-significant between cows with one CL (5.7%) vs. double CL (3.7%). Cavity occurrence was not affected by hormone therapy prior to AI (either PGF2α or OvSynch; 4.4% vs. 5.4%, respectively); however, the number of CL was reduced by the treatments (11.6 vs. 19.6%; P < 0.0005). In twin pregnancies there was no difference in the pregnancy losses between bilateral and unilateral pregnancies at any time point. The length of gestation was 278.2 ± 10.5 (singleton) and 267.4 ± 31.2 (twin) days, respectively (P < 0.01). The stillbirth ratio was higher in twin carriers than in singleton carriers (19.5% vs. 5.3%; P < 0.001).

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  • Andreu-Vázquez, C., García-Ispierto, I., Ganau, S., Fricke, P. M. and López-Gatius, F. (2012): Effects of twinning on the subsequent reproductive performance and productive lifespan of high-producing dairy cows. Theriogenology 78, 20612070.

    • Search Google Scholar
    • Export Citation
  • Ball, P. J. H. (1997): Later embryo and early foetal mortality in the cow. Anim. Breed. Abstr. 65, 167175.

  • Balogh, O. G., Fébel, H., Huszenicza, G., Kulcsár, M., Abonyi-Tóth, Zs., Endrődi, T. and Gábor, G. (2012): Seasonal fertility differences in synchronised dairy cows: ultrasonic, metabolic and endocrine findings. Acta Vet. Hung. 60, 131143.

    • Search Google Scholar
    • Export Citation
  • Balogh, O. G., Túry, E., Abonyi-Tóth, Z., Kastelic, J. and Gábor, G. (2014): Macroscopic and histological characteristics of fluid-filled ovarian structures in dairy cows. Acta Vet. Hung. 62, 215232.

    • Search Google Scholar
    • Export Citation
  • Bech-Sábat, G., López-Gatius, F., García-Ispierto, I., Santolaria, J. P., Serrano, B., Nogareda, C., de Sousa, N. M., Beckers, J. F. and Yániz, J. (2009): Pregnancy patterns during the early foetal period in high producing dairy cows treated with GnRH or progesterone. Theriogenology 71, 920929.

    • Search Google Scholar
    • Export Citation
  • Day, J. D., Weaver, L. D. and Franti, C. E. (1995): Twin pregnancy diagnosis in Holstein cows: Discriminatory powers and accuracy of diagnosis by transrectal palpation and outcome of twin pregnancies. Can. Vet. J. 36, 9397.

    • Search Google Scholar
    • Export Citation
  • Diskin, M. G. and Morris, D. G. (2008): Embryonic and early foetal losses in cattle and other ruminants. Reprod. Dom. Anim. 43 (Suppl. 2), 260–267.

    • Search Google Scholar
    • Export Citation
  • Echternkamp, S. E. and Gregory, K. E. (2002): Effects of twinning on gestation length, retained placenta and dystocia. J. Anim. Sci. 77, 3947.

    • Search Google Scholar
    • Export Citation
  • Fricke, P. M. (2002): Scanning the future –Ultrasonography as a reproductive management tool for dairy cattle. J. Dairy Sci. 85, 19181926.

    • Search Google Scholar
    • Export Citation
  • Gábor, G., Kastelic, J. P., Abonyi-Tóth, Z., Gábor, P., Endrődi, T. and Balogh, O. G. (2016): Pregnancy loss in dairy cattle: relationship of ultrasound, blood pregnancy-specific protein B, progesterone and production variables. Reprod. Domest. Anim. 51, 467473.

    • Search Google Scholar
    • Export Citation
  • Hatvani, C., Balogh, O. G., Endrődi, T., Abonyi-Tóth, Z., Holló, I., Kastelic, J. P. and Gábor, G. (2013): Estrus response and fertility after a single cloprostenol treatment in dairy cows with various ovarian structures. Can. J. Vet. Res. 77, 218220.

    • Search Google Scholar
    • Export Citation
  • Hoedemaker, M., Ruddat, I., Teltscher, M. K., Essmeyer, K. and Kreienbrock, L. (2010): Influence of animal, herd and management factors on perinatal mortality in dairy cattle –A survey in Thuringia, Germany. Berl. Münch. Tierärztl. Wochenschr. 123, 130136.

    • Search Google Scholar
    • Export Citation
  • Hossein-Zadeh, N. G., Nejati-Javaremi, A., Miraei-Ashtiani, S. R. and Kohram, H. (2008): An observational analysis of twin births, calf stillbirth, calf sex ratio, and abortion in Iranian Holsteins. J. Dairy Sci. 91, 41984205.

    • Search Google Scholar
    • Export Citation
  • Humblot, P. (2001): Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. Theriogenology 56, 14171433.

    • Search Google Scholar
    • Export Citation
  • Kastelic, J. P., Curran, S. and Ginther, O. J. (1989): Accuracy of ultrasonography for pregnancy diagnosis on days 10 to 22 in heifers. Theriogenology 31, 813820.

    • Search Google Scholar
    • Export Citation
  • Kirkpatrick, B. W., Byla, B., Kurar, E. and Warren, W. C. (2002): Development of microsatellite markers and comparative mapping for bovine chromosome 19. Anim. Genet. 33, 6568.

    • Search Google Scholar
    • Export Citation
  • Lombard, J. E., Garry, F. B., Tomlinson, S. M. and Garber, L. P. (2007): Impacts of dystocia on health and survival of dairy calves. J. Dairy Sci. 90, 17511760.

    • Search Google Scholar
    • Export Citation
  • Lopez, H., Caraviello, D. Z., Satter, L. D., Fricke, P. M. and Wiltbank, M. C. (2005): Relationship between level of milk production and multiple ovulations in lactating dairy cows. J. Dairy Sci. 88, 27832793.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F. and Hunter, R. H. (2005): Spontaneous reduction of advanced twin embryos: its occurrence and clinical relevance in dairy cattle. Theriogenology 63, 118125.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F., García-Ispierto, I. and Hunter, R. (2010): Factors affecting spontaneous reduction of corpora lutea and twin embryos during the late embryonic/early fetal period in multiple-ovulating dairy cows. Theriogenology 73, 293299.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F., López-Béjar, M., Fenech, M. and Hunter, R. H. (2005): Ovulation failure and double ovulation in dairy cattle: risk factors and effects. Theriogenology 63, 12981307.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F., Santolaria, P., Yániz, J. L., Garbayo, J. M. and Hunter, R. H. (2004): Timing of early foetal loss for single and twin pregnancies in dairy cattle. Reprod. Dom. Anim. 39, 429433.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F., Santolaria, P., Yániz, J., Rutllant, J. and López-Béjar, M. (2002): Factors affecting pregnancy loss from gestation Day 38 to 90 in lactating dairy cows from a single herd. Theriogenology 57, 12511261.

    • Search Google Scholar
    • Export Citation
  • López-Gatius, F., Szenci, O., Bech-Sábat, G., García-Ispierto, I., Serrano, B. and Santolaria, P. and Yániz, J. (2009): Factors of non-infectious nature affecting late embryonic and early foetal loss in high producing dairy herds in north-eastern Spain [with Hungarian abstract]. Magy. Allatorvosok 131, 515531.

    • Search Google Scholar
    • Export Citation
  • Meyer, C. L., Berger, P. J. and Koehler, K. J. (2000): Interactions among factors affecting stillbirths in Holstein cattle in the United States. J. Dairy Sci. 83, 26572663.

    • Search Google Scholar
    • Export Citation
  • Mur-Novales, R., López-Gatius, F., Fricke, P. and Cabrera, V. E. (2018): An economic evaluation of management strategies to mitigate the negative impact of twinning in dairy herds. J. Dairy Sci. 101, 83358349.

    • Search Google Scholar
    • Export Citation
  • National Research Council (2001): Nutrient Requirements of Dairy Cattle. Seventh revised edition, 2001. The National Academies Press, Washington, DC.

    • Export Citation
  • Niles, D. (2016): The modern dairy maternity ward [in English, with Hungarian abstract]. Magy. Allatorvosok 138 (Suppl. 1), 275–279.

  • Norman, H. D., Wright, J. R., Kuhn, M. T., Hubbard, S. M., Cole, J. D. and VanRaden, P. M. (2009): Genetic and environmental factors that affect gestation length in dairy cattle. J. Dairy Sci. 92, 22592269.

    • Search Google Scholar
    • Export Citation
  • Okuda, K., Kito, S., Sumi, N. and Sato, K. (1988): A study of the central cavity in the bovine corpus luteum. Vet. Rec. 123, 180183.

  • Perez-Marín, C. (2009): Formation of corpora lutea and central luteal cavities and their relationship with plasma progesterone levels and other metabolic parameters in dairy cattle. Reprod. Dom. Anim. 44, 384389.

    • Search Google Scholar
    • Export Citation
  • Pieterse, M. C., Szenci, O., Willemse, A. H., Bajcsy, A. C., Dieleman, S. J. and Taverne, M. A. (1990): Early pregnancy diagnosis in cattle by means of linear-array real-time ultrasound scanning of the uterus and a qualitative and quantitative milk progesterone test. Theriogenology 33, 697707.

    • Search Google Scholar
    • Export Citation
  • Saacke, R. G., Dalton, J. C., Nadir, S., Nebel, R. L. and Bame, J. H. (2000): Relationship of seminal traits and insemination time to fertilization rate and embryo quality. Anim. Reprod. Sci. 60–61, 663–677.

    • Search Google Scholar
    • Export Citation
  • Santos, J. E. P., Thatcher, W. W., Chebel, R. C., Cerri, C. L. A. and Galvão, K. N. (2004): The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim. Reprod. Sci. 82–83, 513–535.

    • Search Google Scholar
    • Export Citation
  • Silva-Del-Río, N., Colloton, J. D. and Fricke, P. M. (2009): Factors affecting pregnancy loss for single and twin pregnancies in a high-producing dairy herd. Theriogenology 71, 14621471.

    • Search Google Scholar
    • Export Citation
  • Szenci, O. (1985): Role of acid-base disturbances in perinatal mortality of calves (A summary of thesis). Acta Vet. Hung. 33, 205220.

    • Search Google Scholar
    • Export Citation
  • Szenci, O. (2015): Recent possibilities for the diagnosis and pharmacological control of pregnancy loss in dairy cow. J. Life Sci. 9, 171180.

    • Search Google Scholar
    • Export Citation
  • Szenci, O., Beckers, J. F., Humblot, P., Sulon, J., Sasser, G., Taverne, M. A. M., Varga, J., Baltusen, R. and Schekk, Gy. (1998): Comparison of ultrasonography, bovine pregnancy specific protein B, and bovine pregnancy associated glycoprotein 1 test for pregnancy detection in dairy cows. Theriogenology 50, 7788.

    • Search Google Scholar
    • Export Citation
  • Szenci, O., Gyulai, G., Nagy, P., Kovács, L., Varga, J. and Taverne, M. A. (1995): Effect of uterus position relative to the pelvic inlet on the accuracy of early bovine pregnancy diagnosis by means of ultrasonography. Vet. Q. 17, 3739.

    • Search Google Scholar
    • Export Citation
  • Szenci, O., Humblot, P., Beckers, J. F., Sasser, G., Sulon, J., Baltusen, R., Varga, J., Bajcsy, C. A. and Taverne, M. A. (2000): Plasma profiles of progesterone and conceptus proteins in cows with spontaneous embryonic/foetal mortality as diagnosed by ultrasonography. Vet. J. 159, 287290.

    • Search Google Scholar
    • Export Citation
  • Wiltbank, M. C., Fricke, P. M., Sangsritavong, S., Sartori, R. and Ginther, O. J. (2000): Mechanisms that prevent and produce double ovulations in dairy cattle. J. Dairy Sci. 83, 29983007.

    • Search Google Scholar
    • Export Citation