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  • 1 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary
  • 2 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

We investigated the effect of four feed additives, namely β-glucan, a drinking water acidifier (DWA), a sanguinarine-containing product (SN) and fulvic acid, on hepatic cytochrome P450 (CYP) mRNA expression and CYP enzyme activity in chickens. The test substances were given to the chickens in the recommended dose or in tenfold dose. The administration of 5 mg/kg body weight (bw) β-glucan and 0.1 ml/kg bw DWA for five days decreased the relative gene expression of CYP1A4 and CYP2C23a. The dosing of 50 mg/kg bw β-glucan, 5 and 50 mg/kg bw SN, 1 ml/kg bw DWA and 250 mg/kg bw fulvic acid doubled the hepatic CYP1A4 activity. The activity of CYP2C and CYP3A remained unchanged. Avoidance of CYP1A-mediated feed-drug interactions requires accurate dosing of β-glucan, DWA and fulvic acid. According to our results, no treatment resulted in excessive or less CYP2C and CYP3A protein formation, which reduces the risk of potential feed additive-drug interactions in chickens. However, the administration of feed additive SN containing a plant alkaloid should be avoided concomitantly with CYP1A-metabolised medicines.

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  • Carre, W., Bourneuf, E., Douaire, M. and Diot, C. (2002): Differential expression and genetic variation of hepatic messenger RNAs from genetically lean and fat chickens. Gene 299, 235243. doi: 10.1016/S0378-1119(02)01077-6

    • Search Google Scholar
    • Export Citation
  • Chang, Q., Lu, Z., He, M., Gao, R., Bai, H., Shi, B. and Shan, A. (2014): Effects of dietary supplementation of fulvic acid on lipid metabolism of finishing pigs. J. Anim. Sci. 92, 49214926. doi:https://doi.org/10.2527/jas.2014-8137

    • Search Google Scholar
    • Export Citation
  • Csikó, G., Nagy, G., Mátis, G., Neogrády, Z., Kulcsár, A., Jerzsele, Á., Szekér, K. and Gálfi, P. (2014): Effects of dietary sodium butyrate on hepatic biotransformation and pharmacokinetics of erythromycin in chickens. J. Vet. Pharmacol. Ther. 37, 406412. doi:10.1111/jvp.12109

    • Search Google Scholar
    • Export Citation
  • de Boer, A., van Hunsel, F. and Bast, A. (2015): Adverse food-drug interactions. Regul. Toxicol. Pharmacol. 73, 859865. doi:https://doi.org/10.1016/j.yrtph.2015.10.009

    • Search Google Scholar
    • Export Citation
  • Diani-Moore, S., Papachristou, F., Labitzke, E. and Rifkind, A. B. (2006): Induction of cyp1a and cyp2-mediated arachidonic acid epoxygenation and suppression of 20-hydroxyeicosatetraenoic acid by imidazole derivatives including the aromatase inhibitor vorozole. Drug Metab. Dispos. 34, 1376-1385. doi:10.1124/dmd.106.009498

    • Search Google Scholar
    • Export Citation
  • Fink-Gremmels, J. (2008): Implications of hepatic cytochrome P450-related biotransformation processes in veterinary sciences. Eur. J. Pharmacol. 585, 502509. doi:10.1016/j.ejphar.2008.03.013

    • Search Google Scholar
    • Export Citation
  • Hong, Y. H., Lillehoj, H. S., Lee, S. H., Dalloul, R. A. and Lillehoj, E. P. (2006): Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections. Vet. Immunol. Immunopathol. 114, 209223. doi:http://dx.doi.org/10.1016/j.vetimm.2006.07.007

    • Search Google Scholar
    • Export Citation
  • Islam, K. M. S., Schumacher, A. and Gropp, J. (2005): Humic acid substances in animal agriculture. Pak. J. Nutr. 4, 126134. doi:10.3923/pjn.2005.126.134

    • Search Google Scholar
    • Export Citation
  • Kantas, D., Papatsiros, V. G., Tassis, P. D., Athanasiou, L. V. and Tzika, E. D. (2015): Effect of a natural feed additive (Macleaya cordata), containing sanguinarine, on the performance and health status of weaning pigs. Anim. Sci. J. = Nihon Chikusan Gakkaihō 86, 9298. doi:https://doi.org/10.1111/asj.12240

    • Search Google Scholar
    • Export Citation
  • Nebbia, C., Ceppa, L., Dacasto, M., Nachtmann, C. and Carletti, M. (2001): Oxidative monensin metabolism and cytochrome P450 3A content and functions in liver microsomes from horses, pigs, broiler chicks, cattle and rats. J. Vet. Pharmacol. Ther. 24, 399103. doi:10.1046/j.1365-2885.2001.00362.x

    • Search Google Scholar
    • Export Citation
  • Ourlin, J. C., Baader, M., Fraser, D., Halpert, J. R. and Meyer, U. A. (2000): Cloning and functional expression of a first inducible avian cytochrome P450 of the CYP3A subfamily (CYP3A37). Arch. Biochem. Biophys. 373, 375384. doi:10.1006/abbi.1999.1566

    • Search Google Scholar
    • Export Citation
  • Palócz, O. and Csikó, G. (2014): Reduction of the excessive use of antibiotics in animal husbandry and in clinical practice. Literature review [in Hungarian, with English abstract]. Magy. Allatorvosok 136, 177183.

    • Search Google Scholar
    • Export Citation
  • Palócz, O., Farkas, O., Clayton, P. and Csikó, G. (2017): Changes in cytochrome P450 gene expression and enzyme activity induced by xenobiotics in rabbits in vivo and in vitro. World Rabbit Sci. 25, 173180. doi:10.4995/wrs.2017.4574

    • Search Google Scholar
    • Export Citation
  • Palócz, O., Szita, G. and Csikó, G. (2019): Alteration in inflammatory responses and cytochrome P450 expression of porcine jejunal cells by drinking water supplements. Mediators of Inflammation 2019, 6. doi:https://doi.org/10.1155/2019/5420381

    • Search Google Scholar
    • Export Citation
  • Plaza, C., Garcia-Gil, J. C., Polo, A., Senesi, N. and Brunetti, G. (2005): Proton binding by humic and fulvic acids from pig slurry and amended soils. J. Environ. Qual. 34, 11311137. doi:10.2134/jeq2004.0378

    • Search Google Scholar
    • Export Citation
  • Sridhar, J., Goyal, N., Liu, J. and Foroozesh, M. (2017): Review of ligand specificity factors for CYP1A subfamily enzymes from molecular modeling studies reported to-date. Molecules 22. doi: 10.3390/molecules22071143

    • Search Google Scholar
    • Export Citation
  • Szabó, J., Vucskits, A. V., Berta, E., Andrásofszky, E., Bersényi, A. and Hullár, I. (2017): Effect of fulvic and humic acids on iron and manganese homeostasis in rats. Acta Vet. Hung. 65, 6680. doi:10.1556/004.2017.007

    • Search Google Scholar
    • Export Citation
  • Temesvári, M., Kóbori, L., Paulik, J., Sárváry, E., Belic, A. and Monostory, K. (2012): Estimation of drug-metabolizing capacity by cytochrome P450 genotyping and expression. J. Pharmacol. Exp. Ther. 341, 294305. doi:10.1124/jpet.111.189597

    • Search Google Scholar
    • Export Citation
  • Vieira, S., Berres, J., Reis, R., Oyarzabal, O., Coneglian, J., Freitas, D., Peña, J. and Torres, C. (2008): Studies with sanguinarine like alkaloids as feed additive in broiler diets. Rev. Bras. Cienc. Avic. 10, 6771.

    • Search Google Scholar
    • Export Citation
  • Watanabe, K. P., Kawai, Y. K., Ikenaka, Y., Kawata, M., Ikushiro, S.-I., Sakaki, T. and Ishizuka, M. (2013): Avian cytochrome P450 (CYP) 1-3 family genes: isoforms, evolutionary relationships, and mRNA expression in chicken liver. PloS One 8, e75689. doi:10.1371/journal.pone.0075689

    • Search Google Scholar
    • Export Citation

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