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Réka Borzák Institute for Veterinary Medical Research, Centre for Agricultural Research, P.O. Box 18, Budapest, H-1581, Hungary

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Boglárka Sellyei Institute for Veterinary Medical Research, Centre for Agricultural Research, P.O. Box 18, Budapest, H-1581, Hungary

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Ferenc Baska Department of Exotic Animal and Wildlife Medicine, University of Veterinary Medicine, Budapest, Hungary

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Csaba Székely Institute for Veterinary Medical Research, Centre for Agricultural Research, P.O. Box 18, Budapest, H-1581, Hungary

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Andor Doszpoly Institute for Veterinary Medical Research, Centre for Agricultural Research, P.O. Box 18, Budapest, H-1581, Hungary

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Abstract

Two adult barbels (Barbus barbus) with visible skin tumours were subjected to histopathological and molecular examinations. The fish were caught in the River Danube near Budapest. Papillomas were found around their oral cavity, at the operculum and at the pectoral fins, while epidermal hyperplasias were seen on the body surface. Cyprinid herpesvirus 1 (CyHV-1) was detected in the kidney of the specimens by polymerase chain reaction (PCR), and barbel circovirus 1 (BaCV1) was found in all internal organs and in the tissues of the tumours. The whole genome of BaCV1 and three conserved genes from the genome of CyHV-1 were sequenced. Previously, BaCV1 had been reported only once from a mass mortality event among barbel fry. The whole genome sequence of our circovirus shared 99.9% nucleotide identity with that of the formerly reported BaCV1. CyHV-1 is known to infect common carp and coloured carp (Cyprinus carpio), and has been assumed to infect other cyprinid fish species as well. We found the nucleotide sequences of the genes of CyHV-1 to be identical in 98.7% to those of the previous isolates from carp. To the best of our knowledge, this is the first molecular confirmation of the presence of CyHV-1 DNA in cyprinid fish species other than carp.

Abstract

Two adult barbels (Barbus barbus) with visible skin tumours were subjected to histopathological and molecular examinations. The fish were caught in the River Danube near Budapest. Papillomas were found around their oral cavity, at the operculum and at the pectoral fins, while epidermal hyperplasias were seen on the body surface. Cyprinid herpesvirus 1 (CyHV-1) was detected in the kidney of the specimens by polymerase chain reaction (PCR), and barbel circovirus 1 (BaCV1) was found in all internal organs and in the tissues of the tumours. The whole genome of BaCV1 and three conserved genes from the genome of CyHV-1 were sequenced. Previously, BaCV1 had been reported only once from a mass mortality event among barbel fry. The whole genome sequence of our circovirus shared 99.9% nucleotide identity with that of the formerly reported BaCV1. CyHV-1 is known to infect common carp and coloured carp (Cyprinus carpio), and has been assumed to infect other cyprinid fish species as well. We found the nucleotide sequences of the genes of CyHV-1 to be identical in 98.7% to those of the previous isolates from carp. To the best of our knowledge, this is the first molecular confirmation of the presence of CyHV-1 DNA in cyprinid fish species other than carp.

Introduction

The common barbel (Barbus barbus) belongs to the family Cyprinidae. It is a native freshwater fish in Europe, inhabiting mostly fast-flowing rivers. The barbel is an excellent fish for sporting activities, and its importance in aquaculture is growing.

Little is known about the diseases affecting the barbel, with the exception of a few parasitic (Myxobolus spp., Aspidogaster limacoides, Pomphorhynchus laevis) (Thielen et al., 2004; Molnár et al., 2012) and viral (circovirus, rhabdovirus) infections (Lőrincz et al., 2011; Vicenova et al., 2011). However, recently a large variety of tumours with uncertain origin have been reported from barbels. For example, skin and gonadal tumours have been observed in barbels originating from the River Ohre, Czech Republic (Palikova et al., 2007), and a branchial osteogenetic neoplasm has been reported in specimens from the River Adige, North Italy (Manera and Biavati, 1999). In the River Lee in Southern England, numerous barbels with epidermal hyperplasias and papillomas have been found. Hyperplasias (1–3 mm thick) were found on the scales and papillomas (1–2 cm in diameter) at the base of the fins. They had a cauliflower-like appearance (Barnes et al., 1993). Although viral particles were not observed by electron microscopy, Dixon (2008) surmised that these hyperplasias were due to cyprinid herpesvirus 1 (CyHV-1). In the last few years, several adult barbels with distinct skin tumours were caught in the middle and upper reaches of the Danube close to Budapest during the autumn months. Tumour formation is often considered multifactorial, and viruses, chemical and biological toxins, physical effects, hormones as well as the age, sex, genetic predisposition and immunological competence of the hosts are among the suspected causes (Anders and Yoshimizu, 1994; Roberts, 2012).

Alloherpesviruses (AHVs) are large, enveloped dsDNA viruses infecting fish and amphibians (Davison et al., 2009). The family Alloherpesviridae comprises four genera (Batrachovirus, Salmonivirus, Ictalurivirus and Cyprinivirus). The genus Cyprinivirus contains four virus species accepted by the International Committee on Taxonomy of Viruses (Pellett et al., 2011), three of which (Cyprinid herpesvirus 1, Cyprinid herpesvirus 2, Cyprinid herpesvirus 3) are associated with common carp (Cyprinus carpio) or goldfish (Carassius auratus). The fourth one (Anguillid herpesvirus 1) originated from the European eel (Anguilla anguilla).

CyHV-1 is the causative agent of the so-called carp pox disease in common carp (Sano et al., 1985). Similar clinical signs were reported in other fish species of the family Cyprinidae, including barbel, bleak (Alburnus alburnus), freshwater bream (Abramis brama), chub (Squalius cephalus), Crucian carp (Carassius carassius), ide (Leuciscus idus), roach (Rutilus rutilus) and tench (Tinca tinca) (Mawdesley-Thomas and Bucke, 1967; McAllister et al., 1985; Dixon, 2008); however, it has never been confirmed by molecular methods that these viruses were actually CyHV-1, nor were any of these viruses successfully isolated.

Circoviruses (CVs) are small (12–27 nm) viruses with an icosahedral capsid containing a short single-stranded, covalently closed circular DNA genome (1.7–2.3 kilobases). The Circoviridae is a relatively newly established family of viruses which is rapidly growing as a result of the recent evolution of molecular detection techniques. The family is divided into two genera, Circovirus and Cyclovirus (Breitbart et al., 2017). The very first discovery of fish CVs (barbel circoviruses, BaCV1 and 2) was during a mass mortality event in farmed barbel fry (Lőrincz et al., 2011). Later the presence of CVs was reported in wels catfish (Silurus glanis), European eel (Anguilla anguilla), asp (Aspius aspius), roach and round goby (Neogobius melanostomus) as well (Lőrincz et al., 2012; Doszpoly et al., 2014; Tarján et al., 2014; Borzák et al., 2017).

In the present paper we report the detection of CyHV-1 and BaCV1 by molecular methods in barbels displaying epidermal tumours.

Materials and methods

Specimen collection and pathology

In November of 2014, two specimens of barbel (70 cm, approx. 4 kg) bearing skin tumours were delivered to our laboratory for histopathological and molecular examinations. The fish were caught by a sport fisherman in the River Danube near Budapest. After euthanasia, the fish were necropsied with routine tissue collection from the main organs (skin papilloma, gills, brain, liver, kidney, spleen and intestine) for histopathological, virus isolation and molecular examinations. Tissues were fixed in Bouin's fixative, washed in 80% ethanol, embedded in paraffin, sectioned (at 4–5 µm), stained with haematoxylin and eosin, and viewed by light microscopy according to standard procedures. The barbels were subjected to parasitological examinations as well. In addition, swab samples were collected from the gills and tumours for general microbiological examination on Tryptic Soy Agar (TSA) at 25 °C for 24 h.

Virus isolation

Virus isolation was attempted on EPC (Epithelioma Papulosum Cyprini) cell line (Fijan et al., 1983). The pooled internal organ homogenates were diluted to a 10% (w/v) suspension in MEM medium (Gibco) complemented with antibiotics (Penicillin 300 U/mL, Streptomycin 300 µg/mL). The suspensions were centrifuged at 2000 ×g for 10 min at 16 °C. Inoculations with the supernatant (1 mL suspension per flask) were made in 25-cm2 flasks of EPC monolayer at 80% confluency. The flasks were incubated at 17 °C and 20 °C and checked daily for the appearance of cytopathic effect (CPE).

PCR assays

For the molecular investigations, organ samples were homogenised using the TissueLyser high-throughput disruption instrument (Qiagen, Germany), and the DNA extraction was carried out by DNeasy® Blood and Tissue Kit (Qiagen, Germany) from 25 mg of tissue homogenates according to the manufacturer's recommendations. The samples were tested by two different polymerase chain reactions (PCRs), one developed for the detection of large dsDNA viruses (Hanson et al., 2006) and another one for the amplification of CVs and cycloviruses (Halami et al., 2008). After ascertaining the presence of CyHV-1 and BaCV1, amplification of the whole genome of BaCV1 was attempted by an inverse PCR described previously (Lőrincz et al., 2011). For amplifying longer fragments from the genes of the DNA-dependent DNA polymerase (DNA pol), helicase, and ATPase subunit of the terminase (terminase) of the putative novel CyHV-1, consensus primers designed for cypriniviruses were used (Doszpoly et al., 2015). The PCR products were excised from the agarose gel (1%), purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), and sequenced bidirectionally with the forward and reverse primers. The sequencing PCRs were performed with the use of the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA), and the electrophoresis was carried out by a commercial service provider (SZBK Sequencing Platform, Szeged, Hungary) on an ABI PRISM 3100 Genetic Analyzer. The sequence analysis and contig assembly were performed by the MEGA5.2 software (Tamura et al., 2011).

Results

Gross and microscopic pathology

Macroscopically, the barbels had tumours located around the mouth and the lower jaw, on the operculum, at the pectoral fins, and on the skin/scales throughout the body (Fig. 1). The number of the tumours was less than ten in each barbel. The size of the soft polypoid, pink-coloured masses was found to range between 0.5 and 3 cm in diameter. Additionally, the gross pathological examination revealed intensive infestation with a common acanthocephalan worm (Pomphorhynchus laevis, Müller), many specimens of which were present in the gut of each barbel. By bacteriological examination, only commensal bacteria, ubiquitous in the water environment, could be detected on the surface of gills and tumours. Histopathological examinations of the excised skin tumours revealed that the stroma was well vascularised in its axis. In the benign tumours, the cells were polymorphic. Foci of secondary lymphocytic inflammation were also seen (Fig. 2). The typical herpesvirus-induced nuclear changes including margination of the chromatin and the formation of inclusions (Sano et al., 1985) were not observed. These features are not always present in tissues infected with CyHV-1 (Hedrick et al., 1990). No other significant pathological/histopathological alterations were revealed.

Figure 1.
Figure 1.

External gross pathology. (a, b) Barbel showing papilloma around the oral cavity and at the operculum. (c) Epidermal hyperplasia on the body surface

Citation: Acta Veterinaria Hungarica 68, 1; 10.1556/004.2020.00004

Figure 2.
Figure 2.

Histopathological findings: (a) Cross-sectional view of the papilloma. The stroma is well vascularised in its axis. ST: stroma; V: vessels. Magnification: ×30. (b) Inside the benign tumour, the cells are polymorphic, and some foci of secondary lymphocytic inflammation can also be seen (arrow). Magnification: ×400. Histological section, haematoxylin and eosin (HE)

Citation: Acta Veterinaria Hungarica 68, 1; 10.1556/004.2020.00004

Virus isolation

No CPE was observed during the virus isolation attempts. After 14 days, a blind passage was carried out and then in a fortnight a second blind passage was conducted. There was no CPE in the second and third passages, and even the PCR of the tissue culture supernatants for the detection of viral DNA was negative in the consecutive passages.

PCR assays and sequence analysis

The presence of CyHV-1 was confirmed by the PCR targeting the DNA pol of large dsDNA viruses (Hanson et al., 2006) from the kidney. The other organs did not yield any products by this PCR. Subsequently, the PCRs for obtaining longer sequences from the three conserved genes (Doszpoly et al., 2015) were successful. From the DNA pol gene a 787-bp-long fragment was amplified and sequenced. The PCR for the terminase gene yielded a 942-bp-long fragment, while from the helicase gene a 564-bp-long part was gained. The sequences were deposited to the GenBank (Acc. Nos. MK507841-507843). The nucleotide sequences of the three genes of the CyHV-1 originating from the barbels shared 98.7% nt identity with those of the CyHV-1 described previously from common carp (Davison et al., 2013). Merely one amino acid discrepancy was detected in the terminase gene. The CV was detected in all examined organs of both barbels. The whole genome proved to consist of 1957 nt and shared 99.9% nt and 100 amino acid identity with BaCV1 (Lőrincz et al., 2011).

Discussion

Tumour formation is often considered a multifactorial disorder, resulting from the combined influence of genetic factors, pathogens and environmental insults (Anders and Yoshimizu, 1994; Roberts, 2012). Neoplastic changes at the mouth and pectoral fins have been observed mostly in fish feeding on the bottom. Epidermal injuries could increase the risk of tumour development, since carcinogenic chemicals or oncogenic viruses could find their way easier into the body through an injury than via an intact epithelial surface (Mawdesley-Thomas and Fraser, 1972). Barnes et al. (1993) observed that tumours appeared as epidermal hyperplasias on body surfaces exposed to abrasion, while in the relatively more protected areas the tumours developed into papillomas. In our cases, papillomas were located not only in protected areas but also around the mouth and at the pectoral fins (Fig. 1a) which are more exposed to rubbing, while hyperplasias were observed on the skin/scales throughout the body.

CyHV-1 is a well-known viral pathogen of common carp causing benign epidermal proliferations (Sano et al., 1985), and recently it has also been detected in cutaneous squamous cell carcinoma of koi carp (Sirri et al., 2018). According to current knowledge, herpesviruses have high host specificity (Davison, 2002). Nonetheless, CyHV-1 was described as the putative causative agent of carp-pox-like disease in other cyprinid fish species based on electron microscopic investigations (Mawdesley-Thomas and Bucke, 1967; McAllister et al., 1985; Dixon, 2008). Yet, this has never been confirmed by molecular methods. Very recently, Sellyei et al. (unpublished) have found that carp-pox-like diseases in roach and asp were associated with a novel cyprinid herpesvirus (cyprinid herpesvirus 5); this finding might imply the need for the molecular examination of cyprinid fishes showing the typical signs of the disease.

To the best of our knowledge, this is the first molecular confirmation of the presence of CyHV-1 DNA in fish species other than its usual host, the carp. Without virus isolation and experimental infection, it is not possible to confirm the causal relationship between the tumour development and the presence of CyHV-1. More so, since the virus was detected only in the kidney, and there was no RNA extraction performed for checking the viral replication. However, the seasonality and manifestation of the disease resembled those of CyHV-1 infection in common carp (Hedrick et al., 1990).

CV infection was described in European eel showing signs of the so-called cauliflower disease (benign stomatopapilloma); however, a direct connection between the presence of CV and the disease could not be demonstrated (Doszpoly et al., 2014). Moreover, later the prevalence of eel CV was found to be high (35.5%) in an apparently healthy eel population in Lake Balaton, Hungary (Borzák et al., 2017). The CVs studied in more detail (porcine and avian CVs) are known to have an immunosuppressive effect (Todd, 2004), which may enhance the pathogenicity of some agents causing concomitant infections (e.g. CyHV-1). It is also possible that CVs can cause generalised infection in immunocompromised individuals. Similar conclusions were drawn from the examination of a novel small ssDNA virus in sea turtles with fibropapillomas (Ng et al., 2009). The infection with CVs might also promote the interspecies transmission of the strictly carp-pathogenic virus (CyHV-1). However, it cannot be excluded that water pollution or other predisposing factors also play a role in the manifestation of tumours associated with CyHV-1 and BaCV1 infection in barbels.

Acknowledgements

The excellent technical assistance of Györgyi Ostoros is gratefully acknowledged. This work was supported by a grant provided by the Hungarian Scientific Research Fund (OTKA K127916).

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    • Crossref
    • Search Google Scholar
    • Export Citation
  • Barnes, A., Owen, A. G., Feist, S. W. and Bucke, D. (1993): An investigation into the occurrence of epidermal hyperplasia and papilloma in barbel (Barbus barbus L.) from a river in southern England. Bull. Eur. Assoc. Fish Pathol. 13, 115 118.

    • Search Google Scholar
    • Export Citation
  • Borzák, R., Sellyei, B., Székely, C. and Doszpoly, A. (2017): Molecular detection and genome analysis of circoviruses of European eel (Anguilla anguilla) and sichel (Pelecus cultratus). Acta Vet. Hung. 65, 262 277.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Breitbart, M., Delwart, E., Rosario, K., Segalés, J., Varsani, A. and ICTV Report Consortium. (2017): ICTV virus taxonomy profile: circoviridae. J. Gen. Virol. 98, 1997 1998.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Davison, A. J. (2002): Evolution of the herpesviruses. Vet. Microbiol. 86, 69 88.

  • Davison, A. J., Eberle, R., Ehlers, B., Hayward, G. S., McGeoch, D. J., Minson, A. C., Pellett, P. E., Roizman, B., Studdert, M. J. and Thiry, E. (2009): The order Herpesvirales. Arch. Virol. 154, 171 177.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Davison, A. J., Kurobe, T., Gatherer, D., Cunningham, C., Korf, I., Fukuda, H., Hedrick, R. P. and Waltzek, T. B. (2013): Comparative genomics of carp herpesviruses. J. Virol. 87, 2908 2922.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Dixon, P. F. (2008): Virus diseases of cyprinids. In: Eiras, J. C. and Segner, H. (eds) Fish Diseases, Book 1. Science Publishers, Enfield, Jersey, Plymouth.

    • Search Google Scholar
    • Export Citation
  • Doszpoly, A., Papp, M., Deákné, P. P., Glávits, R., Ursu, K. and Dán, Á. (2015): Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary. Arch. Virol. 160, 1279 1283.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Doszpoly, A., Tarján, Z. L., Glávits, R., Müller, T. and Benkő, M. (2014): Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease. Dis. Aquat. Org. 109, 107 115.

    • Crossref
    • Search Google Scholar
    • Export Citation
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Author information is available in PDF.
Please, download the file from HERE.

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Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 287 7073 (ed.-in-chief) or (36 1) 467 4081 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

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2022  
Web of Science  
Total Cites
WoS
972
Journal Impact Factor 0.900
Rank by Impact Factor

Veterinary Sciences 95/143

Impact Factor
without
Journal Self Cites
0.900
5 Year
Impact Factor
1.1
Journal Citation Indicator 0.47
Rank by Journal Citation Indicator

Veterinary Sciences 103/170

Scimago  
Scimago
H-index
38
Scimago
Journal Rank
0.277
Scimago Quartile Score

Veterinary (miscellaneous) Q2

Scopus  
Scopus
Cite Score
1.9
Scopus
CIte Score Rank
General Veterinary 76/186 (59th PCTL)
Scopus
SNIP
0.475

2021  
Web of Science  
Total Cites
WoS
1040
Journal Impact Factor 0,959
Rank by Impact Factor Veterinary Sciences 103/144
Impact Factor
without
Journal Self Cites
0,876
5 Year
Impact Factor
1,222
Journal Citation Indicator 0,48
Rank by Journal Citation Indicator Veterinary Sciences 106/168
Scimago  
Scimago
H-index
36
Scimago
Journal Rank
0,313
Scimago Quartile Score Veterinary (miscellaneous) (Q2)
Scopus  
Scopus
Cite Score
1,7
Scopus
CIte Score Rank
General Veterinary 79/183 (Q2)
Scopus
SNIP
0,610

2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
submission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
Publication Model Hybrid
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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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