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  • 1 Department of Veterinary Medicine, Via Celoria 10, Milan, Italy
  • 2 Veterinary Teaching Hospital, Via dell’Università 6, Lodi, Italy
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Abstract

In view of the enormous variability of dog breeds, breed-specific reference intervals (RIs) are recommended for use in veterinary clinical decision-making. The aim of this study was to determine whether RIs of the general canine population may be applied to the Italian Greyhound (Piccoli Levrieri Italiani or PLI), and to generate breed-specific RIs, where appropriate. Sixty-three privately owned clinically healthy fasted dogs were examined. Routine haematology and biochemistry were performed on 58 enrolled patients using the ADVIA 120 haematology analyzer and the Cobas Mira system, respectively. Changes in haematological and biochemical parameters depending on sex, age and attitude (resting vs. running dogs) were investigated. The results of PLI were compared with the RIs of the general canine population. In those cases in which these RIs were not validated, new RIs were generated according to the guidelines of the American Society of Veterinary Clinical Pathology. Pre-existing RIs were considered valid based on the recommendations by the Clinical & Laboratory Standards Institute (CLSI). RIs were higher for mean corpuscular haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), cell haemoglobin concentration mean (CHCM) and lower for large unstained cells (LUC). A wider discrepancy between pre-existing and newly established RIs was found for some ADVIA parameters regarding red blood cell (RBC) or reticulocyte morphology. For total protein and cholesterol the new RIs were wider than the pre-existing ones, while albumin, calcium and iron were higher. This study suggests that most of the RIs published in veterinary textbooks cannot be validated for PLIs.

  • Campora, C., Freeman, K. P., Lewis, F. I., Gibson, G., Sacchini, F. and Sanchez-Vazquez, M. J. (2011a): Determination of haematological reference intervals in healthy adult Greyhounds. J. Small Anim. Pract. 52 , 301309.

    • Search Google Scholar
    • Export Citation
  • Campora, C., Freeman, K. P., Serra, M. and Sacchini, F. (2011b): Reference intervals for Greyhounds and Lurchers using the Sysmex XT-2000iV hematology analyzer. Vet. Clin. Pathol. 40, 467474.

    • Search Google Scholar
    • Export Citation
  • Caro, J. T., Marín, L. M., Iazbik, M. C., Zaldivar-López, S., Borghese, H., Couto, C. G. (2013): Markers of iron metabolism in retired racing Greyhounds with and without osteosarcoma. Vet. Clin. Pathol. 42, 360363.

    • Search Google Scholar
    • Export Citation
  • CLSI (2010): Defining, Establishing and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline. Third ed. CLSI document EP28-A3c. Clinical and Laboratory Standards Institute, Wayne, PA.

    • Search Google Scholar
    • Export Citation
  • Couto, C. G., Cerón, J. J., Parra, M. D., Martìnez-Subiela, S. and Iazbik, M. C. (2009): Acute phase protein concentrations in retired racing Greyhounds. Vet. Clin. Pathol. 38, 219223.

    • Search Google Scholar
    • Export Citation
  • Fayos, M., Couto, C. G., Iazbik, M. C. and Wellman, M. L. (2005): Serum protein electrophoresis in retired racing Greyhounds. Vet. Clin. Pathol. 34, 397400.

    • Search Google Scholar
    • Export Citation
  • Friedrichs, K. R., Harr, K. E., Freeman, K. P., Szladovits, B., Walton, R. M., Barnhart, K. F., Blanco-Chavez, J. and American Society for Veterinary Clinical Pathology (2012): ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Vet. Clin. Pathol. 41, 441453.

    • Search Google Scholar
    • Export Citation
  • Furlanello, T., Tasca, S., Caldin, M., Carli, E., Patron, C., Tranquillo, M., Lubas, G. and Solano-Gallego, L. (2006): Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer. Vet. Clin. Pathol. 35, 4246.

    • Search Google Scholar
    • Export Citation
  • Geffré, A., Concordet, D., Braun, J. P. and Trumel, C. (2011): Reference value advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel. Vet. Clin. Pathol. 40, 107112.

    • Search Google Scholar
    • Export Citation
  • Geffré, A., Friedrichs, K., Harr, K., Concordet, D., Trumel, C. and Braun, J. P. (2009): Reference values: a review. Vet. Clin. Pathol. 38, 288298.

    • Search Google Scholar
    • Export Citation
  • Giori, L., Gironi, S., Scarpa, P., Anselmi, A., Gualtieri, M. and Paltrinieri, S. (2011): Grey eosinophils in sighthounds: frequency in 3 breeds and comparison of eosinophil counts determined manually and with 2 hematology analyzers. Vet. Clin. Pathol. 40, 475483.

    • Search Google Scholar
    • Export Citation
  • Horn, P. S. and Pesce, A. J. (2002): Effect of ethnicity on reference intervals. Clin. Chem. 48, 18021804.

  • Horvath, S. J., Couto, C. G., Yant, K., Kontur, K., Bohenko, L., Iazbik, M. C., Marin, L. M., Hudson, D., Chase, J., Frye, M. and Denicola, D. B. (2014): Effects of racing on reticulocyte concentrations in Greyhounds. Vet. Clin. Pathol. 43, 1523.

    • Search Google Scholar
    • Export Citation
  • Ilkiw, J. E., Davis, P. E. and Church, D. B. (1989): Hematologic, biochemical, blood-gas, and acid-base values in greyhounds before and after exercise. Am. J. Vet. Res. 50, 583586.

    • Search Google Scholar
    • Export Citation
  • Kaneko, J. J., Harvey, J. W. and Bruss, M. L. (2008): Appendix IX. In: Kaneko, J. J., Harvey, J. W. and Bruss, M. L. (eds) Clinical Biochemistry of Domestic Animals. Sixth ed. Academic Press, San Diego, CA. pp. 898899.

    • Search Google Scholar
    • Export Citation
  • Kenyon, C. L., Basaraba, R. J. and Bohn, A. A. (2011): Influence of endurance exercise on serum concentrations of iron and acute phase proteins in racing sled dogs. J. Am. Vet. Med. Assoc. 239, 12011210.

    • Search Google Scholar
    • Export Citation
  • Kjelgaard-Hansen, M. and Lundorff, J. A. (2010): Reference intervals. In: Weiss, D. J. and Wardrop, K. J. (eds) Schalm's Veterinary Hematology. Sixth ed. Wiley-Blackwell Publishing, Ames, IA. pp. 10341038.

    • Search Google Scholar
    • Export Citation
  • Lassen, E. D., Craig, A. M. and Blythe, L. L. (1986): Effects of racing on hematologic and serum biochemical values in Greyhounds. J. Am. Vet. Med. Assoc. 188 , 12991303.

    • Search Google Scholar
    • Export Citation
  • Mahmoud, M. Y., Lugon, M. and Anderson, C. C. (1996): Unexplained macrocytosis in elderly patients. Age Ageing 25 , 310312.

  • Moritz, A. and Becker, M. (2010): Automated hematology systems. In: Weiss, D. J. and Wardrop, K. J. (eds) Schalm's Veterinary Hematology. Sixth ed. Wiley-Blackwell Publishing, Ames, IA. pp. 10541066.

    • Search Google Scholar
    • Export Citation
  • Moritz, A., Fickenscher, Y. and Meyer, K. (2004): Canine and feline hematology reference values for the ADVIA 120 hematology system. Vet. Clin. Pathol. 33, 3238.

    • Search Google Scholar
    • Export Citation
  • Nielsen, L., Kjelgaard-Hansen, M., Jensen, A. L. and Kristensen, A. T. (2010): Breed-specific variation of hematologic and biochemical analytes in healthy adult Bernese Mountain dogs. Vet. Clin. Pathol. 39, 2028.

    • Search Google Scholar
    • Export Citation
  • Paltrinieri, S., Ibba, F. and Rossi, G. (2014): Haematological and biochemical reference intervals of four feline breeds. J. Feline Med. Surg. 16, 125136.

    • Search Google Scholar
    • Export Citation
  • Rizzi, T. E., Meinkoth, J. H. and Clinkenbeard, K. D. (2010): Normal hematology of the dog. In: Weiss, D. J. and Wardrop, K. J. (eds) Schalm's Veterinary Hematology. Sixth ed. Wiley-Blackwell Publishing, Ames, IA. pp. 799810.

    • Search Google Scholar
    • Export Citation
  • Ruggerone, B., Giraldi, M., Paltrinieri, S. and Scarpa, P. (2018): Hematologic and biochemical reference intervals in Shetland Sheepdog. Vet. Clin. Pathol. 47, 617624.

    • Search Google Scholar
    • Export Citation
  • Shiel, R. E., Brennan, S. F., O’Rourke, L. G., McCullough, M. and Mooney, C. T. (2007): Hematologic values in young pretraining healthy Greyhounds. Vet. Clin. Pathol. 36, 274277.

    • Search Google Scholar
    • Export Citation
  • Stockham, S. L. and Scott, M. A. (2008): Calcium, phosphorus, magnesium and their regulatory hormones. In: Fundamentals of Veterinary Clinical Pathology. Second ed. Blackwell Publishing, Ames, IA. pp. 593602.

    • Search Google Scholar
    • Export Citation
  • Toll, P. W., Gaehtgens, P., Neuhaus, D., Pieschi, R. L. and Fedde, M. R. (1995): Fluid, electrolyte, and packed cell volume shifts in racing greyhounds. Am. J. Vet. Res. 56, 227232.

    • Search Google Scholar
    • Export Citation
  • Tvedten, H. and Moritz, A. (2010): Reticulocyte and Heinz body staining and enumeration. In: Weiss, D. J. and Wardrop, K. J. (eds) Schalm’s Veterinary Hematology. Sixth ed. Wiley-Blackwell Publishing, Ames, IA. pp. 10671073.

    • Search Google Scholar
    • Export Citation
  • Uhrikova, I., Lacnakova, A., Tandlerova, K., Kucharova, V., Rehakova, K., Janova, E. and Doubek, J. (2013): Haematological and biochemical variations among eight sighthound breeds. Aust. Vet. J. 91, 452459.

    • Search Google Scholar
    • Export Citation
  • Zaldívar-López, S., Marín, L. M., Iazbik, M. C., Westendorf-Stingle, N., Hensley, S. and Couto, C. G. (2011): Clinical pathology of Greyhounds and other sighthounds. Vet. Clin. Pathol. 40, 414425.

    • Search Google Scholar
    • Export Citation

  • Impact Factor (2018): 1.059
  • Veterinary (miscellaneous) SJR Quartile Score (2018): Q2
  • Scimago Journal Rank (2018): 0.517
  • SJR Hirsch-Index (2018): 30

Language: English

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      Varga, János
      Štefan, Vilĉek (Slovak Republic)
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      Zsarnovszky, Attila

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