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  • 1 Hungarian Academy of Sciences Institute for Veterinary Medical Research, Centre for Agricultural Research Budapest Hungary
  • | 2 Kuwait University College of Graduate Studies, Molecular Biology Program Kuwait Kuwait
  • | 3 Kuwait University Department of Microbiology, Faculty of Medicine Kuwait Kuwait
  • | 4 Hungarian Defence Forces Dr. Radó György Military Medical Centre Budapest Hungary
  • | 5 University of Pécs Department of Medical Microbiology and Immunology Pécs Hungary
  • | 6 University of Bari Department of Animal Health and Wellbeing Bari Italy
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In this study a Kuwaiti camel rotavirus strain, RVA/Camel-wt/KUW/s21/2010/G10P[15], is characterized by sequencing and phylogenetic analysis. The strain had multiple genes with high nucleotide sequence similarities to ovine and bovine strains (VP2, ≤ 96%; NSP2 and NSP5, ≤ 97%, NSP3, ≤ 94%), or, to porcine strains (VP1, ≤ 89%). Other genes had moderate sequence similarities (VP4, ≤ 87%; VP6, ≤ 81%; VP7, ≤ 82%) with reference strains from ruminants. The NSP4 gene shared limited sequence identity (≤ 71%) with other mammalian and avian rotavirus NSP4 types, and was designated a novel genotype, E15. This study demonstrates genetic diversity in the outer capsid and some backbone genes of an old-world camelid rotavirus strain and uncovers its common evolutionary roots with strains from other ruminants.

  • Matthijnssens, J., Ciarlet, M., McDonald, S. M., Attoui, H., Bányai, K., Brister, J. R., Buesa, J., Esona, M. D., Estes, M. K., Gentsch, J. R., Iturriza-Gómara, M., Johne, R., Kirkwood, C. D., Martella, V., Mertens, P. P., Nakagomi, O., Parreño, V., Rahman, M., Ruggeri, F. M., Saif, L. J., Santos, N., Steyer, A., Taniguchi, K., Patton, J. T., Desselberger, U., Van Ranst, M.: Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156, 1397–1413 (2011).

    Ranst M. , 'Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG) ' (2011 ) 156 Arch Virol : 1397 -1413.

    • Search Google Scholar
  • Matthijnssens, J., Ciarlet, M., Rahman, M., Attoui, H., Bányai, K., Estes, M. K., Gentsch, J. R., Iturriza-Gómara, M., Kirkwood, C. D., Martella, V., Mertens, P. P., Nakagomi, O., Patton, J. T., Ruggeri, F. M., Saif, L. J., Santos, N., Steyer, A., Taniguchi, K., Desselberger, U., Van Ranst, M.: Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153, 1621–1629 (2008).

    Ranst M. , 'Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments ' (2008 ) 153 Arch Virol : 1621 -1629.

    • Search Google Scholar
  • Martella, V., Bányai, K., Matthijnssens, J., Buonavoglia, C., Ciarlet, M.: Zoonotic aspects of rotaviruses. Vet Microbiol 140, 246–255 (2010)

    Ciarlet M. , 'Zoonotic aspects of rotaviruses ' (2010 ) 140 Vet Microbiol : 246 -255.

  • Abo Hatab, E. M., Hussein, H. A., El-Sabagh, I. M., Saber, M. S.: Isolation and antigenic and molecular characterization of G10 of group A rotavirus in camel. Int J Virol 5, 18–27 (2009).

    Saber M. S. , 'Isolation and antigenic and molecular characterization of G10 of group A rotavirus in camel ' (2009 ) 5 Int J Virol : 18 -27.

    • Search Google Scholar
  • Al-Mutairi, L. Z.: Detection and genotyping camel rotaviruses in Kuwait. Thesis, Kuwait University (2011).

  • Maes, P., Matthijnssens, J., Rahman, M., Van Ranst, M.: RotaC: A web-based tool for the complete genome classification of group A rotaviruses. BMC Microbiol 9, 238 (2009).

    Ranst M. , 'RotaC: A web-based tool for the complete genome classification of group A rotaviruses ' (2009 ) 9 BMC Microbiol : 238 -.

    • Search Google Scholar
  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731–2739 (2011).

    Kumar S. , 'MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods ' (2011 ) 28 Mol Biol Evol : 2731 -2739.

    • Search Google Scholar
  • Shen, S., Burke, B., Desselberger, U.: Nucleotide sequences of the VP4 and VP7 genes of a Chinese lamb rotavirus: evidence for a new P type in a G10 type virus. Virology 197, 497–500 (1993).

    Desselberger U. , 'Nucleotide sequences of the VP4 and VP7 genes of a Chinese lamb rotavirus: evidence for a new P type in a G10 type virus ' (1993 ) 197 Virology : 497 -500.

    • Search Google Scholar
  • Chen, Y., Zhu, W., Sui, S., Yin, Y., Hu, S., Zhang, X.: Whole genome sequencing of lamb rotavirus and comparative analysis with other mammalian rotaviruses. Virus Genes 38, 302–310 (2009).

    Zhang X. , 'Whole genome sequencing of lamb rotavirus and comparative analysis with other mammalian rotaviruses ' (2009 ) 38 Virus Genes : 302 -310.

    • Search Google Scholar
  • Esona, M. D., Mijatovic-Rustempasic, S., Conrardy, C., Tong, S., Kuzmin, I. V., Agwanda, B., Breiman, R. F., Banyai, K., Niezgoda, M., Rupprecht, C. E., Gentsch, J. R., Bowen, M. D.: Reassortant group A rotavirus from straw-colored fruit bat (Eidolon helvum). Emerg Infect Dise 16, 1844–1852 (2010).

    Bowen M. D. , 'Reassortant group A rotavirus from straw-colored fruit bat (Eidolon helvum) ' (2010 ) 16 Emerg Infect Dise : 1844 -1852.

    • Search Google Scholar
  • Esona, M. D., Geyer, A., Banyai, K., Page, N., Aminu, M., Armah, G. E., Hull, J., Steele, D. A., Glass, R. I., Gentsch, J. R.: Novel human rotavirus genotype G5P[7] from child with diarrhea, Cameroon. Emerg Infect Dis 15, 83–86 (2009).

    Gentsch J. R. , 'Novel human rotavirus genotype G5P[7] from child with diarrhea, Cameroon ' (2009 ) 15 Emerg Infect Dis : 83 -86.

    • Search Google Scholar
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