View More View Less
  • 1 Hungarian Academy of Sciences, Budapest, Hungary
  • 2 National Food Chain Safety Office, Budapest, Hungary
  • 3 Szent István University, Budapest, Hungary
Full access

Bovine respiratory disease (BRD) is the leading cause of significant economic losses in the intensive beef industry worldwide. Beside numerous risk factors Pasteurella multocida, which is regarded as a secondary pathogen, may play a role in the development of the disease. Previous studies of strains from swine pneumonia revealed that there are a few clones associated with clinical disease, suggesting that some strains may be more virulent than others. This linkage may be true in the BRD, however composition of P. multocida populations in the herds are slightly characterized. Thus, we decided to perform phenotypic and genotypic characterisation of strains isolated from calves with respiratory infection at 31 different herds in Hungary. The results demonstrated the presence of two dominant strain types. At the identical taxonomic background (P. multocida subsp. multocida) with slight phenotypic variability they could be separated by trehalose fermentation capacity, α-glucosidase activity and molecular fingerprint patterns of ERIC- and M13-PCR. Independent prevalence and geographical origin of the strain types may refer to their significance in the illness, but their comparison with strains isolated from healthy individuals is taken into consideration.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1.

    Ózsvári, L., Muntyán, J., Berkes, Á.: Financial losses caused by bovine respiratory disease (BRD) in cattle herds. MÁL 5, 259264 (2012).

    • Search Google Scholar
    • Export Citation
  • 2.

    Carter, G. R.: Observations on the pathology and bacteriology of shipping fever in Canada. Can J Comp Med Vet Sci 18, 359364 (1954).

  • 3.

    Nikunen, S., Härtel, H., Orro, T., Neuvonen, E., Tanskanen, R., Kivelä, S. L., Sankari, S., Aho, P., Pyörälä, S., Saloniemi, H., Soveri, T.: Association of bovine respiratory disease with clinical status and acute phase proteins in calves. Comp Immunol Microbiol Infect Dis 30, 143151 (2007).

    • Search Google Scholar
    • Export Citation
  • 4.

    Pardon, B., De Bleecker, K., Dewulf, J., Callens, J., Boyen, F., Catry, B., Deprez, P.: Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. Vet Rec 169, 278 (2011).

    • Search Google Scholar
    • Export Citation
  • 5.

    Welsh, R. D., Dye, L. B., Payton, M. E., Confer, A. W.: Isolation and antimicrobial susceptibilities of bacterial pathogens from bovine pneumonia: 1994–2002. J Vet Diagn Invest 16, 426431 (2004).

    • Search Google Scholar
    • Export Citation
  • 6.

    Sadler, D. F, Ezzell, J. W. Jr, Keller, K. F., Doyle, R. J.: Glycosidase activities of Bacillus anthracis. J Clin Microbiol 19, 594598 (1984).

    • Search Google Scholar
    • Export Citation
  • 7.

    Blackall, P. J., Pahoff, J. L., Bowles, R.: Phenotypic characterization of Pasteurella multocida isolates from Australian pigs. Vet Microbiol 57, 355360 (1997).

    • Search Google Scholar
    • Export Citation
  • 8.

    Fegan, N., Blackall, P. J., Pahoff, J. L.: Phenotypic characterization of Pasteurella multocida isolates from Australian poultry. Vet Microbiol 47, 281286 (1995).

    • Search Google Scholar
    • Export Citation
  • 9.

    Sellyei, B., Varga, Z., Pné Samu, K., Magyar, T.: Characterisation of Pasteurella multocida strains isolated from rabbits. MÁL 130, 396403 (2008).

    • Search Google Scholar
    • Export Citation
  • 10.

    Varga, Zs., Sellyei, B., Magyar, T.: Phenotypic and genotypic characterisation of Pasteurella multocida strains isolated from pigs in Hungary. Acta Vet Hung 55, 425434 (2007).

    • Search Google Scholar
    • Export Citation
  • 11.

    Townsend, K. M., Frost, A. J., Lee, C. W.: Genetic organisation of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39, 924929 (2001).

    • Search Google Scholar
    • Export Citation
  • 12.

    Heddleston, K. L., Gallager, J. E., Rebers, P. A.: Fowl cholera: Gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis 16, 925936 (1972).

    • Search Google Scholar
    • Export Citation
  • 13.

    Sellyei, B., Bányai, K., Magyar, T.: Characterization of the ptfA gene of avian Pasteurella multocida strains by allele specific polymerase chain reaction. J Vet Diagn Invest 22, 607610 (2010).

    • Search Google Scholar
    • Export Citation
  • 14.

    Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M., Dawkins, H. J.: Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates. J Clin Microbiol 36, 10961100 (1998).

    • Search Google Scholar
    • Export Citation
  • 15.

    Sellyei, B., Wehmann, E., Magyar, T.: Sequencing-independent method for the differentiation of the main phylogenetic lineages of Pasteurella multocida. J Vet Diagn Invest 24, 735738 (2012).

    • Search Google Scholar
    • Export Citation
  • 16.

    Versalovic, J., Koeuth, T., Lupski, J. R.: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucl Acids Res 19, 68236831 (1991).

    • Search Google Scholar
    • Export Citation
  • 17.

    Dabo, S. M., Confer, A. W., Lu Y. S.: Single primer polymerase chain reaction fingerprinting for Pasteurella multocida isolates from laboratory rabbits. Am J Vet Res 61, 305309 (2000).

    • Search Google Scholar
    • Export Citation
  • 18.

    Taylor, J. D., Fulton, R. W., Dabo, S. M., Lehenbauer, T. W., Confer, A. W.: Comparison of genotypic and phenotypic characterization methods for Pasteurella multocida isolates from fatal cases of bovine respiratory disease. J Vet Diagn Invest 22, 366375 (2010).

    • Search Google Scholar
    • Export Citation
  • 19.

    Van De Peer, Y., De Wachter, Y.: TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Applic Biosci 10, 569570 (1994).

    • Search Google Scholar
    • Export Citation
  • 20.

    Hotchkiss, E. J., Hodgson, J. C., Schmitt-van de Leemput, E., Dagleish, M. P., Zadoks, R. N.: Molecular epidemiology of Pasteurella multocida in dairy and beef calves. Vet Microbiol 151, 329335 (2011).

    • Search Google Scholar
    • Export Citation
  • 21.

    Taylor, J. D.: Molecular epidemiology of Pasteurella multocida respiratory disease in beef cattle. PhD thesis, Oklahoma State University Center for Veterinary Health Sciences, 2008, pp. 1140.

    • Search Google Scholar
    • Export Citation
  • 22.

    Ross, R. F.: Pasteurella multocida and its role in porcine pneumonia. Anim Health Res Rev 7, 1329 (2007).

  • 23.

    Hunt Gerardo, S., Citron, D. M., Claros, M. C., Fernandez, H. T., Goldstein, E. J.: Pasteurella multocida subsp. multocida and Pasteurella multocida subsp. septica differentiation by PCR fingerprinting and a-glucosidase activity. J Clin Microbiol 39, 25582564 (2001).

    • Search Google Scholar
    • Export Citation
  • 24.

    Sellyei, B., Varga, Z., Ivanics, E., Magyar, T.: Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays. Acta Vet Hung 56, 429440 (2008).

    • Search Google Scholar
    • Export Citation

  • Impact Factor (2018): 1.079
  • Immunology and Microbiology (miscellaneous) SJR Quartile Score (2018): Q4
  • Medicine (miscellaneous) SJR Quartile Score (2018): Q3
  • Scimago Journal Rank (2018): 0.313
  • SJR Hirsch-Index (2018): 24
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • SCOPUS
  • Science Citation Index Expanded

Language: English

Founded in 1954
Size: B5
Publication: One volume of four issues annually
Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Global Health
  • Index Medicus

 

Subscribers can access the electronic version of every printed article.

Senior editors

Editor(s)-in-Chief: Szabó, Dóra

Managing Editor(s): Kocsis, Béla

Co-editor(s): Horváth, Andrea

Editorial Board

      Ádám, É. (Budapest)
      S. Amyes (Edinburgh)
      K. Burián (Szeged) (Pécs)
      O. Dobay (Budapest)
      I. Duany (Magdeburg)
      L. Emődy (Pécs)
      A. Erdei (Budapest)
      É. M. Fenyő (Lund)
      L. Fodor (Budapest)
      J. Kónya (Debrecen)
      Y. Mándy (Szeged)
      K. Márialigeti (Budapest)
      J. Minárovits (Szeged)
      B. Nagy (Budapest)
      I. Nász (Budapest)
      K. Nékám (Budapest)
      E. Ostorházi (Budapest)
      R. Pusztai (Szeged)
      P. Rády (Houston)
      É. Rajnavölgyi (Debrecen)
      F. Rozgonyi (Budapest)
      Zs. Schaff (Budapest)
      J. G. Sinkovics (Tampa)
      J. Szekeres (Pécs)
      M. Takács (Budapest)
      E. Urbán (Szeged)

Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: (36 1) 210 2930 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu

The author instruction is available in PDF.
Please, download the file from HERE

 

MANUSCRIPT SUBMISSION