Portulaca oleracea L. (Portulacaceae), although an alien, has turned to be a very useful plant in Indian folk medicine. The present work deals with the evaluation of the antifungal activity of this plant. Fungitoxicity of aqueous and organic solvent (e.g. hexane, ethanol and chloroform) extracts were tested against Aspergillus niger, Rhizopus artocarpi and Fusarium sp. by Agar Cup Assay and Filter Disc methods. Hexane and aqueous extracts show antifungal activity against Fusarium sp., while ethanol and chloroform extracts of the same herb inhibit the growth of Rhizopus artocarpi. These findings can have practical application.