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  • 1 Semmelweis University 2nd Department of Obstetrics and Gynecology, Faculty of Medicine Budapest Hungary
  • 2 Semmelweis University Szentágothai János Knowledge Center Budapest Hungary
  • 3 Eötvös Lóránd University Department of Immunology, Institute of Biology Budapest Hungary
  • 4 Hungarian Academy of Sciences Research Group of Inflammation Biology and Immunogenomics Budapest Hungary
  • 5 Helmholtz Center of Infection Research and Lionex Diagnostics and Therapeutics Ltd Department of Genome Analysis Braunschweig Germany
  • 6 Semmelweis University 3rd Department of Medicine, Faculty of Medicine Budapest Hungary
  • 7 Semmelweis University 2nd Department of Obstetrics and Gynecology Faculty of Medicine Üllői út 78/A H-1083 Budapest Hungary
Open access

Anti-human Hsp60 autoantibodies — known risk factor of atherosclerosis — were investigated in a mouse model and in samples of healthy subjects: polyreactivity, presence in cord blood samples of healthy newborns and life-long stability were tested. In IgM hybridoma panel from mouse spleens, polyreactivity of anti-Hsp60 autoantibodies was studied. In healthy pregnant women, umbilical vein and maternal blood samples were collected after childbirth, anti-Hsp-60 and -65 IgM and IgG levels were measured. Life-long stability of anti-Hsp-60 levels was studied on healthy patients during 5 years. ELISA was used in all studies. Polyreactivity of IgM clones of newborn mice and lifelong stability of these autoantibodies in healthy adults were established. IgM anti-Hsp60 autoantibodies in cord blood of healthy human infants were present, however, there was no correlation between maternal and cord blood IgM anti-Hsp60 concentrations. It is proposed that presence of anti-Hsp60 autoantibodies — as part of the natural autoantibody repertoire — may be an inherited trait. Level of anti-Hsp60 autoantibodies may be an independent, innate risk factor of atherosclerosis for the adulthood.

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