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  • 1 University of Chile Departamento de Producci ón Agricola Casilla, Santiago 1004 Chile
  • 2 Escola Agrotécnica Federal de Sombrio Caixa Postal 04 Santa Rosa de Sul Brazil
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Grapevine fanleaf virus (GFLV) is the causal agent of a widespread disease that affects vineyards. Since it is difficult to culture viruses, the availability of an easy and efficient method of virus maintenance in the laboratory would be of interest to virologists. The objective of this research was to determine an adequate culture medium that promotes callus growth and permits the preservation of GFLV on Vitis vinifera tissue. Fragments of in vitro cultured leaves (25 mm 2 ), originated from Cabernet Sauvignon positive for GFLV, were cultivated on a Murashige and Skoog (1962) medium, and the callus was monitored for the presence of GFLV every two weeks using ELISA. Higher 2,4-D concentration induced a higher growth, particularly when combined with a low BA concentration. The medium enriched with 1.0 ppm of 2,4-D combined with 0.5 ppm of BA showed the best result, with the callus area reaching more than 250 mm 2 after 8 weeks in culture. ELISA absorbance observed on callus tissues during the whole period was, at least, three times higher than that observed on leaves positive for GFLV kept either in vivo or in vitro and more than 18 times higher than that of the negative control. Any remarkable difference in absorbance was recorded during the period of callus cultivation. It was concluded that the viral load on the callus was not affected during this time, suggesting that this kind of in vitro culture is an efficient method to preserve GFLV.

  • Bos, L. (1977): Persistence of infectivity of three viruses in plant material dried over CaCl and stored under different conditions. Neth. J. Plant Path. 83, 217–220

    Bos L. , 'Persistence of infectivity of three viruses in plant material dried over CaCl and stored under different conditions ' (1977 ) 83 Neth. J. Plant Path. : 217 -220.

    • Search Google Scholar
  • Carrington, J., Kasschau, K., Mahajan, S. and Schaad, M. (1996): Cell-to-cell and long-distance transport of viruses in plants. The Plant Cell 8, 1669–1681.

    Schaad M. , 'Cell-to-cell and long-distance transport of viruses in plants ' (1996 ) 8 The Plant Cell : 1669 -1681.

    • Search Google Scholar
  • Chen, P., Buss, G., Tolin, S. A. and Veilleux, R. E. (2003): A tissue culture methods for the preservation of soybean mosaic virus strains. Plant Cell, Tissue and Organ Culture 74, 185–192.

    Veilleux R. E. , 'A tissue culture methods for the preservation of soybean mosaic virus strains ' (2003 ) 74 Plant Cell, Tissue and Organ Culture : 185 -192.

    • Search Google Scholar
  • Clark, M. and Adams, A. (1977): Characteristics of the microplate method of enzyme-linked inmunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475–483.

    Adams A. , 'Characteristics of the microplate method of enzyme-linked inmunosorbent assay for the detection of plant viruses ' (1977 ) 34 Journal of General Virology : 475 -483.

    • Search Google Scholar
  • De Wijs, J. J. and Suda-Bachmann, F. (1979): The long-term preservation of Potato virus Y and Water - melon mosaic virus in liquid nitrogen in comparison to other preservation methods. Eur. J. of Plant Pathol. 85, 23–29

    Suda-Bachmann F. , 'The long-term preservation of Potato virus Y and Water-melon mosaic virus in liquid nitrogen in comparison to other preservation methods ' (1979 ) 85 Eur. J. of Plant Pathol. : 23 -29.

    • Search Google Scholar
  • Duran-Vila, N., Cambra, M., Medina, V., Ortega, C. and Navarro, L. (1989): Growth and morphogenesis of citrus tissue cultures infected with citrus tristeza virus and citrus infection variegation virus. Phytopathology 79, 820–826.

    Navarro L. , 'Growth and morphogenesis of citrus tissue cultures infected with citrus tristeza virus and citrus infection variegation virus ' (1989 ) 79 Phytopathology : 820 -826.

    • Search Google Scholar
  • Hollings, M. and Stone, O. M. (1970): The long-term survival of some viruses preserved by lyophilization. Ann. Appl. Biol. 65, 411–418

    Stone O. M. , 'The long-term survival of some viruses preserved by lyophilization ' (1970 ) 65 Ann. Appl. Biol. : 411 -418.

    • Search Google Scholar
  • Infante, R., Mazzara, M. and Rosati, P. (1998): Growth estimation of in vitro cultured callus and plant regeneration from leaf disk and petiole callus of musk strawberry (F ragaria moschata duch.) subcultured for 18 months. J. of the Japanese Society for Horticultural Science 67, 39–43.

    Rosati P. , 'Growth estimation of in vitro cultured callus and plant regeneration from leaf disk and petiole callus of musk strawberry (Fragaria moschata duch.) subcultured for 18 months ' (1998 ) 67 J. of the Japanese Society for Horticultural Science : 39 -43.

    • Search Google Scholar
  • Lazarowitz, S. and Beachy, R. (1999): Viral movement protein as probes for intracellular and intercellular trafficking in plants. The Plant Cell 11, 535–548.

    Beachy R. , 'Viral movement protein as probes for intracellular and intercellular trafficking in plants ' (1999 ) 11 The Plant Cell : 535 -548.

    • Search Google Scholar
  • Mckinney, H. H. and Silber, G. (1968): Methods of preservation and storage of plant viruses. In: Maramorosch, K. and Koprowski, H. (eds): Methods in Virology Vol. 4 (pp. 491–501). Academic Press, New York.

    Silber G. , '', in Methods in Virology Vol. 4 , (1968 ) -.

  • Murashige, T. and Skoog, F. (1962): A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Plant Physiol. 15, 473–497.

    Skoog F. , 'A revised medium for rapid growth and bio-assays with tobacco tissue cultures ' (1962 ) 15 Plant Physiol : 473 -497.

    • Search Google Scholar
  • Navas-Castillo, J., Moreno, P. and Duran-Vila, N. (1995): Citrus psorosis, ringspot, cristacortis and gum pathogens are maintaned in callus culture. Plant Cell, Tissue and Organ Culture 40, 133–137.

    Duran-Vila N. , 'Citrus psorosis, ringspot, cristacortis and gum pathogens are maintaned in callus culture ' (1995 ) 40 Plant Cell, Tissue and Organ Culture : 133 -137.

    • Search Google Scholar
  • Pelah, D., Altman, A. and Czosneck, H. (1994): Tomato yellow leaf curl virus DNA in callus cultures derived from infected tomato leaves. Plant Cell, Tissue and Organ Culture 39, 37–42.

    Czosneck H. , 'Tomato yellow leaf curl virus DNA in callus cultures derived from infected tomato leaves ' (1994 ) 39 Plant Cell, Tissue and Organ Culture : 37 -42.

    • Search Google Scholar
  • Ritzenthaler, C., Schmit, A., Michler, P., Stussi-garaud, C. and Pinck, L. (1995): Grapevine fanleaf nepovirus p38 putative movement protein is located on tubules in vivo . Molecular Plant-Microbe Interactions 8, 379–387.

    Pinck L. , 'Grapevine fanleaf nepovirus p38 putative movement protein is located on tubules in vivo ' (1995 ) 8 Molecular Plant-Microbe Interactions : 379 -387.

    • Search Google Scholar
  • Sampol, B., Bota, J., Riera, D., Medrano, H. and Flexas, J. (2003): Analysis of the virus-induced inhibition of photosinthesis in malmsey grapevines. New Phytologist 160, 403–412.

    Flexas J. , 'Analysis of the virus-induced inhibition of photosinthesis in malmsey grapevines ' (2003 ) 160 New Phytologist : 403 -412.

    • Search Google Scholar
  • Seung-Heui, K. and Seon-Kyu, K. (2002): Effects of auxins and cytokinins on callus induction from leaf blade, petiole, and stem segments of in vitro grown “Sheridan ” grape shoots. Journal of Plant Biotechnology 4, 17–21.

    Seon-Kyu K. , 'Effects of auxins and cytokinins on callus induction from leaf blade, petiole, and stem segments of in vitro grown “Sheridan ” grape shoots ' (2002 ) 4 Journal of Plant Biotechnology : 17 -21.

    • Search Google Scholar
  • Scholthof, H. (2005): Plant virus transport: motions of functional equivalence. Plant Science 10, 376–382.

    Scholthof H. , 'Plant virus transport: motions of functional equivalence ' (2005 ) 10 Plant Science : 376 -382.

    • Search Google Scholar
  • Taylor, R. J. and Secor, G. A. (1992): Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth. Environmental and Experimental Botany 32, 43–48.

    Secor G. A. , 'Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth ' (1992 ) 32 Environmental and Experimental Botany : 43 -48.

    • Search Google Scholar
  • Valat, L., Burrus, M., Fuchs, M. and Mauro, M. C. (2003): Review of techniques to inoculate grapevines with Grapevine fanleaf virus: Lessons and Perspectives. Am. J. Enol. Vitic. 54:4, 279–285

    Mauro M. C. , 'Review of techniques to inoculate grapevines with Grapevine fanleaf virus: Lessons and Perspectives ' (2003 ) 54 Am. J. Enol. Vitic. : 279 -285.

    • Search Google Scholar
  • Walkey, D. G. A. (1985): Storage of virus isolates. In: Walkey, D. G. A. (ed.): Applied Plant Virology (pp. 107–108). Chapman and Hall, London, New Cork.

    Walkey D. G. A. , '', in Applied Plant Virology , (1985 ) -.