Acta Phytopathologica et Entomologica Hungarica
Volume/Issue:
Volume 54: Issue 1
Screening some Common Molecular Markers and a Desaturase Marker, Linked to Sex Pheromone Biosynthesis, in Three Z-Strain and an E-Strain Populations of the European Corn Borer, Ostrinia nubilalis, Occurring in Central Europe
Authors:
G. Bozsik 1Department of Zoology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1022 Budapest, Herman Ottó út 15. Hungary

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A. Lakatos 1Department of Zoology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1022 Budapest, Herman Ottó út 15. Hungary

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G. Szőcs 1Department of Zoology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1022 Budapest, Herman Ottó út 15. Hungary

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I. Tóbiás 2Department of Pathophysiology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1022 Budapest, Herman Ottó út 15. Hungary

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Pages:
127–136
Online Publication Date:
Jun 2019
Publication Date:
01 Jun 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/038.54.2019.911
Keywords:
sex pheromone strains; cryptic sibling species; maize; pest; moth; diagnostic DNA marker; early warning; larval stage
CORRECTION TO THIS ARTICLE
Open access

The European corn borer moth, (ECB), Ostrinia nubilalis (Lepidoptera: Crambidae, Pyraustinae) is one of the most destructive pests of maize worldwide. ECB has two pheromone-strains, separated by specific ratios of isomers of E- and Z11-tetradecenyl acetates (E11- and Z11-14Ac), but appearing morphologically identical. Accordingly, E- and Z-ECB pheromone traps are available for the respective populations for practical monitoring of the flight, however, traps for Z-strain are unreliable for practical usage in some parts of Central-Europe. E- and Z-ECB populations occur in sympatry in some areas, while in allelopatry in other areas. Determining the strains before the flight of adults, when difference in the composition of their respective pheromones is manifested, would be of practical interest for early warning. In addition to the known fatty-acyl-reductase (FAR) marker, further markers would allow more comprehensive studies. We screened the following common markers for mitochondrial and nuclear DNA regions: partial cytochrome c oxidase I (COI), cytochrome B (CytB), the second spacer of the internal transcribed spacer (ITS2), Elongation factor 1a (EF1a) and actin gene (Act). In addition, a marker of the Δ11-desaturase gene (11desat), linked to biosynthesis of female-produced sex pheromone, was also included, because we reported earlier a differential expression for this site. Three Z-ECB populations locating at distant sites within Hungary, an area where only Z-strain occurs, and an E-ECB population in Slovenia, known as the closest-occurring E-strain, were included into the study. Separate laboratory colonies were established from each population, and F1 generations were sampled to verify the identity of pheromone strains, by analysing the composition of sex pheromone by gas chromatography linked to an electroantennographic detector (GC-EAD). Molecular studies were conducted using specimens taken from the F2 generations. Results of genetic studies showed that there were no differences between the Z and E populations for the common markers. In contrast to this, several nucleic acid changes (11 nt in 4 positions) were found between the three Z-populations (Hungary) and the E-population (Slovenia) in the desaturase marker. Further study is required to reveal whether the differences found in this study are consistent across E-populations, thus making these markers suitable for diagnostic purposes.

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Cover Acta Phytopathologica et Entomologica Hungarica
Acta Phytopathologica et Entomologica Hungarica
Print ISSN:
0238-1249
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1588-2691

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Z BOZSÓ Centre for Agricultural Research, Hungary
PE CHETVERIKOV Saint-Petersburg State University, Russia
JX CUI Henan Institute of Science and Technology, China
J FODOR Centre for Agricultural Research, Hungary
Z IMREI Centre for Agricultural Research, Hungary
BM KAYDAN Çukurova University, Turkey
L KISS University of Southern Queensland, Australia
V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary
MW NEGM Ibaraki University, Japan
L PALKOVICS Széchenyi István University, Hungary
M POGÁNY Centre for Agricultural Research, Hungary
D RÉDEI National Chung Hsing University, Taiwan
A TOLSTIKOV University of Tyumen, Russia
J VUTS Rothamsted Research, UK
GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
Language English
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Foundation		 1966
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