View More View Less
  • 1 Institute of Veterinary Research of Athens, N.AG.RE.F. Neapoleos 25, Aghia Paraskevi GR 153 10 Athens Greece
  • 2 Trion Ierarchon 7 GR 118 51 Thisseio Athens Greece
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

Characterisation of unifloral honeys is a hard task that involves evaluation of the results of melissopalynological, physicochemical and sensory analyses. Finding reliable chemical markers to ascertain botanical origin of honey is of great importance to the beekeeping industry. The purpose of this work was to analyse untreated Greek thyme honeys and evaluate the possibility of establishing chemical markers for this honey by using a simple and reliable analytical method. The analytical method employed consists of extraction on octadecylsilica cartridges, GC separation and FID detection. The limits of detection were 30, 20, 15 and 15 μg kg −1 for eucalyptol, camphor, menthol and thymol, respectively, while the limit of quantification for each substance was 50 μg kg −1 . Overall recoveries were >85%. The results showed that none of the 47 thyme honey samples analysed contained eucalyptol or camphor. All samples contained menthol and thymol, but the concentrations were very low ranging from traces to 51.3 μg kg −1 and 65 μg kg −1 , respectively.

  • Bogdanov, S., Ruoff, K. & PERSANO ODDO , L. (2004): Physico-chemical methods for the characterization of unifloral honeys: a review. Apidologie , 35 , S4–S17.

    Persano Oddo L. , 'Physico-chemical methods for the characterization of unifloral honeys: a review ' (2004 ) 35 Apidologie : S4 -S17.

    • Search Google Scholar
  • EMEA (European Medicines Agency ) (1995): Menthol Summary Report. EMEA/MRL/045/95-FINAL.

  • EMEA (European Medicines Agency ) (1996): Thymol Summary Report. EMEA/MRL/075/96-FINAL.

  • EMEA (European Medicines Agency ) (1999): Camphora Summary Report. EMEA/MRL/684/99-FINAL.

  • European Commission (2002): Opinion of the Scientific Committee on Food on eucalyptol. SCF/CS/FLAV/FLAVOUR/20 ADD2 Final.

  • Floris, I., Satta, A., Cabras, P., Gatau, V.L. & Angioni, A. (2004): Comparison between two thymol formulations in the control of Varroa destructor : effectiveness, persistence, and residues. J. econ. Entomology , 97 , 187–191.

    Angioni A. , 'Comparison between two thymol formulations in the control of Varroa destructor: effectiveness, persistence, and residues ' (2004 ) 97 J. econ. Entomology : 187 -191.

    • Search Google Scholar
  • Guyot, C., Bouseta, A., Scheirman, V. & Collin, S. (1998): Floral origin markers of chestnut and lime tree honeys. J. agric. Fd Chem. , 46 , 625–633.

    Collin S. , 'Floral origin markers of chestnut and lime tree honeys ' (1998 ) 46 J. agric. Fd Chem. : 625 -633.

    • Search Google Scholar
  • Li, M., Nelson, D.L. & Sporns, P. (1993): Determination of menthol in honey by gas chromatography. J. A.O.A.C. int. , 76 , 1289–1295.

    Sporns P. , 'Determination of menthol in honey by gas chromatography ' (1993 ) 76 J. A.O.A.C. int. : 1289 -1295.

    • Search Google Scholar
  • Lodesani, M., Pellacani, A., Bergomi, S., Carpana, E., Rabitti, T. & Lasagni, P. (1992): Residue determination for some products used against Varroa infestation in bees. Apidologie , 23 , 257–272.

    Lasagni P. , 'Residue determination for some products used against Varroa infestation in bees ' (1992 ) 23 Apidologie : 257 -272.

    • Search Google Scholar
  • Mattila, H.R. & Otis, G.W. (1999): Trials of Apiguard, a thymol-based miticide. Part 1. Efficacy for control of parasitic mites and residues in honey. Am. Bee J. , 139 , 947–952.

    Otis G.W. , 'Trials of Apiguard, a thymol-based miticide. Part 1. Efficacy for control of parasitic mites and residues in honey ' (1999 ) 139 Am. Bee J. : 947 -952.

    • Search Google Scholar
  • Nozal, M.J., Bernal, J.L., Jimenez, J.J., Gonzalez, M.J. & Hoges, M. (2002): Extraction of thymol, eucalyptol, menthol, and camphor residues from honey and beeswax. Determination by gas chromatography with flame ionization detection. J. Chromat. A , 954 , 207–215.

    Hoges M. , 'Extraction of thymol, eucalyptol, menthol, and camphor residues from honey and beeswax. Determination by gas chromatography with flame ionization detection ' (2002 ) 954 J. Chromat. A : 207 -215.

    • Search Google Scholar
  • Odeh, I., Abu-Lafi, S., Dewik, H., Al-Najjar, I., Imam, A., Dembitsky, V.M. & Hanus, L.O. (2007): A variety of volatile compounds as markers in Palestinian honey from Thymus capitatus, Thymelaea hirsuta , and Tolpis virgata . Fd Chem. , 101 , 1393–1397.

    Hanus L.O. , 'A variety of volatile compounds as markers in Palestinian honey from Thymus capitatus, Thymelaea hirsuta, and Tolpis virgata ' (2007 ) 101 Fd Chem. : 1393 -1397.

    • Search Google Scholar
  • Soria, A.C., Gonzalez, M. , De Lorenzo, C., Martinez-Castro, I. & Sanz, J. (2004): Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopalynological, physicochemical and volatile composition data. Fd Chem. , 85 , 121–130.

    Sanz J. , 'Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopalynological, physicochemical and volatile composition data ' (2004 ) 85 Fd Chem. : 121 -130.

    • Search Google Scholar
  • Tsigouri, A., Passaloglou-Katrali, M. & Sabatakou, Ï. (2004): Palynological characteristics of different unifloral honeys from Greece. Grana , 43 , 122–128.

    Sabatakou , 'Palynological characteristics of different unifloral honeys from Greece ' (2004 ) 43 Grana : 122 -128.

    • Search Google Scholar
  • Vinas, P., Soler-Romera, M.J. & Hernandez-Cordoba, M. (2006): Liquid chromatographic determination of phenol, thymol and carvacrol in honey using fluorimetric detection. Talanta , 69 , 1063–1067.

    Hernandez-Cordoba M. , 'Liquid chromatographic determination of phenol, thymol and carvacrol in honey using fluorimetric detection ' (2006 ) 69 Talanta : 1063 -1067.

    • Search Google Scholar
  • Adamczyk, S., Lazaro, R., Perez-Arquillue, C., Conchello, P. & Herrera, A. (2005): Evaluation of residues of essential oil components in honey after different anti-varroa treatments. J. agric. Fd Chem. , 53 , 10085–10090.

    Herrera A. , 'Evaluation of residues of essential oil components in honey after different anti-varroa treatments ' (2005 ) 53 J. agric. Fd Chem. : 10085 -10090.

    • Search Google Scholar
  • Bogdanov, S., Imdorf, A. & Kilchenmann, V. (1998): Residues in wax and honey after Apilife VAR® treatment. Apidologie , 29 , 513–524.

    Kilchenmann V. , 'Residues in wax and honey after Apilife VAR® treatment ' (1998 ) 29 Apidologie : 513 -524.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2020 9 0 0
Nov 2020 5 0 0
Dec 2020 6 0 0
Jan 2021 12 0 0
Feb 2021 6 0 0
Mar 2021 46 0 0
Apr 2021 0 0 0