Six monocultivar virgin olive oils (VOOs) produced from five autochthonous (Buža, Buža Puntoža, Istarska Bjelica, Porečka Rosulja, and Rosinjola) and one referent cultivar (Leccino), were investigated. The mass fractions of orthodiphenols (ORT) and tocopherols (TOC) were analysed by VIS spectroscopy and HPLC. The radical-scavenging capacity was evaluated by electron spin resonance spectroscopy (ESR) using galvinoxyl free radical and the DPPH test. Results revealed a high level of total TOC in VOOs of Buža Puntoža (243 mg kg−1) and Porečka Rosulja (325 mg kg−1). VOOs contained in 100 g from 79% (Istarska Bjelica) to 261% (Porečka Rosulja) of recommended daily allowance of α-tocopherol. The mass fraction of ORT in Istarska Bjelica and Rosinjola was >250 mg kg−1 (on average 30–40% higher in comparison to other studied cultivars). Istarska Bjelica and Porečka Rosulja showed the highest antioxidant capacity in both methods of measurement. The highest capacity to inhibit protein carbonylation (PC) in response to oxidative stress (54–56%) was displayed by Buža Puntoža, Leccino, and Buža. High level of positive linear correlation between ORT mass fraction and radical-scavenging capacity measured by DPPH test (r=0.768), as well as strong negative correlation between PC inhibition and mass fraction of ORT (r= −0.697), were observed.
APARICIO, R. & LUNA, G. (2002): Characterisation of monovarietal virgin olive oils. Eur. J. Lipid Sci. Tech., 104, 614–627.
BALDIOLI, M., SERVILI, M., PERRETTI, G. & MONTEDORO, G. (1996): Antioxidant activity of tocopherols and phenolic compounds in virgin olive oil. J. Am. Oil Chem. Soc., 73, 1589–1593.
BENDINI, A., CERRETANI, L., CARRASCO-PANCORBO, A., GÓMEZ-CARAVACA, A.M., SEGURA-CARRETERO, A., FERNÁNDEZGUTIÉRREZ, A. & LERCKER, G. (2007): Phenolic molecules in virgin olive oils: A survey of their sensory properties, health effects, antioxidant activity and analytical methods. An overview of the last decade. Molecules, 12, 1679–1719.
BOSKOU, D.; TSIMIDOU, M. & BLEKAS, D. (2006): {bdOlive oil, chemistry and technology}. {plAOCS Press}, {rsChampaign, USADOI}, p. {su44}.
BOSKOU, D. (2009): Phenolic compounds in olives and olive oil. -in: BOSKOU, D. (Ed.) Olive oil minor constituents and health. CRC Press, Boca Raton, USA, pp. 11–44.
CARRASCO-PANCORBO, A., CERRETANI, L., BENDINI, A., SEGURA-CARRETERO, A., DEL CARLO, M., GALLINA-TOSCHI, T., LERCKER, G., COMPAGNONE, D. & FERNÁNDEZ-GUTIÉREZ, A. (2005): Evaluation of the antioxidant capacity of individual phenolic compounds in virgin olive oil. J. Agr. Food Chem., 53, 8918–8925.
CASTEGNA, A., DRAKE, J., POCEMICH, C. & BUTTERFIELD, D.A. (2003): Protein carbonyl levels –An assessment of protein oxidation. -in: HENSLEY, K. & FLOYD, R.A. (Eds) Methods in biological oxidative stress: Methods in pharmacology and toxicology. Humana Press Inc., Totowa, NJ, pp. 161–168.
EC (2006): Regulation no. 1924/2006, O. J. L 404, p1 of 30 December 2006.
EC (2012): Regulation no. 432/2012, O. J. L 136, p1 of 16 May 2012.
EEC (1991): Regulation no. 2568/1991, O. J. L 248, p1 of 11 July 1991.
GUTIÉRREZ, F., JÍMENEZ, B., RUÍZ, A. & ALBI, M.A. (1999): Effect of olive ripeness on the oxidative stability of virgin olive oil extracted from the varieties Picual and Hojiblanca and on the different components involved. J. Agr. Food Chem., 47, 121–127.
HUANG, D., OU, B. & PRIOR, R.L. (2005): The chemistry behind antioxidant capacity assays. J. Agr. Food Chem., 53, 1841–1856.
IOC (2007). Colorimetric method for the determination of o-diphenolic compound in olive oils. International Olive Council. Available at: http://www.internationaloliveoil.org/estaticos/view/224-testing-methods (last accessed: 4 December 2014)
ISO (1997): Animal and vegetable fats and oils –determination of tocopherols and tocotrienols contents –method using high-performance liquid chromatography. ISO International Standard no. 9936, International Organization for Standardization, Geneva, Switzerland.
KALANTZAKIS, G., BLEKAS, G., PEGKILDOU, K. & BOSKOU, D. (2006): Stability and radical-scavenging activity of heated olive oil and other vegetable oils. Eur. J. Lipid. Sci. Tech., 108, 329–335.
KOPRIVNJAK, O., VRHOVNIK, I., HLADNIK, T., PRGOMET, Ž.; HLEVNJAK, B. & MAJETIC GERMEK, V. (2012): Characteristics of nutritive value of virgin olive oils from Buža, Istarska Bjelica, Leccino and Rosulja cultivars. Croat. J. Food Technol. Biotechnol., Nutr., 7, 172–178.
PAPADIMITRIOU, V., SOTIROUDIS, T.G., XENAKIS, A., SOFIKITI, N., STAVYIANNOUDAKI, V. & CHANIOTAKIS, N.A. (2006): Oxidative stability and radical scavenging activity of extra virgin olive oils: An electron paramagnetic resonance spectroscopy study. Anal. Chim. Acta, 573, 453–458.
POLJUHA, D., SLADONJA, B., BRKIC BUBOLA, K., RADULOVIC, M., BRŠCIC, K., ŠETIC, E., KRAPAC, M. & MILOTIC, A. (2008): A multidisciplinary approach to the characterisation of autochthonous Istrian olive (Olea europaea L.) varieties. Food Technol. Biotech., 46, 347–354.
TURA, D., GIGLIOTTI, C., PEDÒ, S., FAILLA, O., BASS, D. & SERRAIOCCO, A. (2007): Influence of cultivar and site of cultivation on levels of lipophilic and hydrophilic antioxidants in virgin olive oils (Olea europaea L.) and correlations with oxidative stability. Sci. Hortic., 112, 108–119.
VALK, E.E. & HORNSTRA, G. (2000): Relationship between vitamin E requirement and polyunsaturated fatty acid intake in man: a review. Int. J. Vitam. Nutr. Res., 70, 31–42.
ŽANETIC, M., CERRETANI, L., ŠKEVIN, D., POLITEO, O., VITANOVIC, E., JUKIC ŠPIKA, M., PERICA, S. & OŽIC, M. (2013): Influence of polyphenolic compounds on the oxidative stability of virgin olive oils from selected autochthonous varieties. J. Food Agric. Environ., 11, 126–131.