Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100T revealed by de novo genome project data

Á. Tóth; E. Baka; Sz. Luzics; I. Bata-Vidács; I. Nagy; B. Bálint; R. Herczeg; F. Olasz; T. Wilk; T. Nagy; B. Kriszt; I. Nagy; J. Kukolya

doi:10.1556/066.2016.0014

Acta Alimentaria

Volume/Issue:: Volume 46: Issue 3

Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100^T revealed by de novo genome project data

Authors:

Á. Tóth

Á. Tóth Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó út 15. Hungary

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E. Baka

E. Baka Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó út 15. Hungary

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Sz. Luzics

Sz. Luzics Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó út 15. Hungary

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I. Bata-Vidács

I. Bata-Vidács Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó út 15. Hungary

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I. Nagy

I. Nagy Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62. Hungary
SeqOmics Biotechnology Ltd., H-6782 Mórahalom, Vállalkozók útja 7. Hungary

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B. Bálint

B. Bálint SeqOmics Biotechnology Ltd., H-6782 Mórahalom, Vállalkozók útja 7. Hungary

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R. Herczeg

R. Herczeg SeqOmics Biotechnology Ltd., H-6782 Mórahalom, Vállalkozók útja 7. Hungary

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F. Olasz

F. Olasz Laboratory of Microbiology; Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, H-2100 Gödöllő, Szent-Györgyi Albert utca 4. Hungary

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T. Wilk

T. Wilk Laboratory of Microbiology; Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, H-2100 Gödöllő, Szent-Györgyi Albert utca 4. Hungary

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T. Nagy

T. Nagy SeqOmics Biotechnology Ltd., H-6782 Mórahalom, Vállalkozók útja 7. Hungary

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B. Kriszt

B. Kriszt Department of Environmental Safety and Ecotoxicology, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, H-2100 Gödöllő, Páter Károly utca 1. Hungary

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I. Nagy

I. Nagy Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18., D-82152 Martinsried. Germany

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J. Kukolya

J. Kukolya Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó út 15. Hungary

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Pages:: 323–335

Online Publication Date:: Sep 2017

Publication Date:: 01 Sep 2017

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/066.2016.0014

Keywords:: de novo genome project; glycoside hydrolyses; cellulases; hemicellulases; amylases

Open access

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Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100^T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100^T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

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Acta Alimentaria

Print ISSN:: 0139-3006

Online ISSN:: 1588-2535

Editorial Board

László Abrankó, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Tamás Antal, University of Nyíregyháza, Nyíregyháza, Hungary
Diána Bánáti, University of Szeged, Szeged, Hungary
József Baranyi, Institute of Food Research, Norwich, UK
Ildikó Bata-Vidács, Eszterházy Károly Catholic University, Eger, Hungary
Ferenc Békés, FBFD PTY LTD, Sydney, NSW Australia
György Biró, Budapest, Hungary
Anna Blázovics, Semmelweis University, Budapest, Hungary
Francesco Capozzi, University of Bologna, Bologna, Italy
Marina Carcea, Research Centre for Food and Nutrition, Council for Agricultural Research and Economics Rome, Italy
Zsuzsanna Cserhalmi, Budapest, Hungary
Marco Dalla Rosa, University of Bologna, Bologna, Italy
István Dalmadi, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Katarina Demnerova, University of Chemistry and Technology, Prague, Czech Republic
Mária Dobozi King, Texas A&M University, Texas, USA
Muying Du, Southwest University in Chongqing, Chongqing, China
Sedef Nehir El, Ege University, Izmir, Turkey
Søren Balling Engelsen, University of Copenhagen, Copenhagen, Denmark
Éva Gelencsér, Budapest, Hungary
Vicente Manuel Gómez-López, Universidad Católica San Antonio de Murcia, Murcia, Spain
Jovica Hardi, University of Osijek, Osijek, Croatia
Hongju He, Henan Institute of Science and Technology, Xinxiang, China
Károly Héberger, Research Centre for Natural Sciences, ELKH, Budapest, Hungary
Nebojsa Ilić, University of Novi Sad, Novi Sad, Serbia
Dietrich Knorr, Technische Universität Berlin, Berlin, Germany
Hamit Köksel, Hacettepe University, Ankara, Turkey
Katia Liburdi, Tuscia University, Viterbo, Italy
Meinolf Lindhauer, Max Rubner Institute, Detmold, Germany
Min-Tze Liong, Universiti Sains Malaysia, Penang, Malaysia
Marena Manley, Stellenbosch University, Stellenbosch, South Africa
Miklós Mézes, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary
Áron Németh, Budapest University of Technology and Economics, Budapest, Hungary
Perry Ng, Michigan State University, Michigan, USA
Quang Duc Nguyen, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Laura Nyström, ETH Zürich, Switzerland
Lola Perez, University of Cordoba, Cordoba, Spain
Vieno Piironen, University of Helsinki, Finland
Alessandra Pino, University of Catania, Catania, Italy
Mojmir Rychtera, University of Chemistry and Technology, Prague, Czech Republic
Katharina Scherf, Technical University, Munich, Germany
Regine Schönlechner, University of Natural Resources and Life Sciences, Vienna, Austria
Arun Kumar Sharma, Department of Atomic Energy, Delhi, India
András Szarka, Budapest University of Technology and Economics, Budapest, Hungary
Mária Szeitzné Szabó, Budapest, Hungary
Sándor Tömösközi, Budapest University of Technology and Economics, Budapest, Hungary
László Varga, Széchenyi István University, Mosonmagyaróvár, Hungary
Rimantas Venskutonis, Kaunas University of Technology, Kaunas, Lithuania
Barbara Wróblewska, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olsztyn, Poland

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Acta Alimentaria
Language	English
Size	B5
Year of Foundation	1972
Volumes per Year	1
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