View More View Less
  • 1 Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rkp. 3, Hungary
Open access

In existing processes, the extraction of steviol glycosides from stevia leaves involves many process steps often including extraction by organic solvents. The purpose of the present study was to develop a process for the effective extraction of steviol glycosides, which can provide a concentrated juice exhibiting a high level of recovery with regard to the target compounds, rebaudioside A and stevioside. Pressurized Hot Water Extraction (PHWE) was first optimized with Response Surface Methodology in terms of maximized rebaudioside-A yield and minimized colour components. PHWE was then combined with pressing in a wine-press, resulting higher efficiency for extracting both steviol glycosides in comparison to the reported methods in the literature. Finally, spray drying was applied for both product stabilization and removal of contaminants.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Chhaya, K., Sharma, C., Mondal, S., Majumdar, G.C. & De, S. (2012): Clarification of stevia extract by ultrafiltration: Selection criteria of the membrane and effects of operating conditions. Food Bioprod. Process., 90, 525532.

    • Search Google Scholar
    • Export Citation
  • Erkucuk, A., Akgun, I.H. & Yesil-Celiktas, O. (2009): Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J. Supercrit. Fluid., 51, 2935.

    • Search Google Scholar
    • Export Citation
  • Henderson, J.W. & Berry, J. (2009): Isocratic stevia sweetener analysis using selective ZORBAX columns. Application note — Agilent. Available at: https://www.agilent.com/cs/library/applications/5990-3933EN.pdf (Last accessed: 07.01.2017)

    • Search Google Scholar
    • Export Citation
  • Jaitak, V., Bandna, B.S. & Kaul, V.K. (2009): An efficient microwave-assisted extraction process of stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni). Phytochem. Analysis, 20, 240245.

    • Search Google Scholar
    • Export Citation
  • Kearney, J. (2010): Food consumption trends and drivers. Philos. T. R. Soc. B., 365, 27932807.

  • Kolb, N., Herrera, J.L., Ferreyra, D.J. & Uliana, R.F. (2001): Analysis of sweet diterpene glycosides from Stevia rebaudiana: Improved HPLC method. J. Agr. Food Chem., 49, 45384541.

    • Search Google Scholar
    • Export Citation
  • Kumari, N., Rana, R.C., Sharma Y.P. & Kumar, S. (2017): Extraction, purification and analysis of sweet compounds in Stevia rebaudiana Bertoni using chromatographic techniques. Indian J. Pharm. Sci., 79, 617624.

    • Search Google Scholar
    • Export Citation
  • Kutowy, O., Zhang, Q. S. & Kumar, A. (1999): Extraction of sweet compounds from Stevia rebaudiana Bertoni, US Patent No. 5972120

  • , K.-A., Robin, F. & Roger, O. (2016): Sugar replacers: from technological challenges to consequences on health. Curr. Opin. Clin. Nutr., 19(4), 310315.

    • Search Google Scholar
    • Export Citation
  • Lemus-Mondaca, R., Vega-Galvez, A., Zura-Bravo, L. & Ah-Hen, K. (2012): Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem., 132, 11211132.

    • Search Google Scholar
    • Export Citation
  • Liu, J., Li, J.-W. & Tang, J. (2010): Ultrasonically assisted extraction of total carbohydrates from Stevia rebaudiana Bertoni and identification of extracts. Food Bioprod. Process., 88, 215221.

    • Search Google Scholar
    • Export Citation
  • Makapugay, H.C., Nanayakkara, N.P.D. & Kinghorn, A.D. (1984): Improved high-performance liquid chromatographic separation of the Stevia rebaudiana sweet diterpene glycosides using linear gradient elution. J. Chromatogr. A, 283, 390395.

    • Search Google Scholar
    • Export Citation
  • Markosyan, A. (2012): Food ingredients from Stevia rebaudiana: US 20120329884 A1, Google Patents.

  • Mathur, S., Bulchandani, N., Parihar, S. & Shekhawat, G.S. (2017): Critical review on steviol glycosides: Pharmacological, toxicological and therapeutic aspects of high potency zero caloric sweetener. Int. J. Pharmacol., 13, 916928.

    • Search Google Scholar
    • Export Citation
  • Plaza, M. & Turner, C. (2015): Pressurized hot water extraction of bioactives. TrAC-Trend. Anal. Chem., 71, 3954.

  • Pól, J., Varaďová Ostrá, E., Karásek, P., Roth, M., Benešová, K., Kotlaříková, P. & Čáslavský, J. (2007): Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana: Methanol versus water. Anal. Bioanal. Chem., 388, 18471857.

    • Search Google Scholar
    • Export Citation
  • Puri, M., Sharma, D., Barrow, C.J. & Tiwary, A.K. (2012): Optimisation of novel method for the extraction of steviosides from Stevia rebaudiana leaves. Food Chem., 132, 11131120.

    • Search Google Scholar
    • Export Citation
  • Rajab, R., Mohan, K.C., Murugan, K., Harish, M. & Mohanan, P.V. (2009): Purification and toxicity studies of stevioside from Stevia rebaudiana Bertoni. Toxicology International, 16, 4954.

    • Search Google Scholar
    • Export Citation
  • Rao, A.B., Reddy, G.R., Ernala, P., Sridhar, S. & Ravikumar, Y.V.L. (2012): An improvised process of isolation, purification of steviosida Bertoni leaves and its biological activity. Int. J. Food Sci. Tech., 47, 25542560.

    • Search Google Scholar
    • Export Citation
  • Serfaty, M., Ibdah, M., Fischer, R., Chaimovitsh, D., Saranga, Y. & Dudai, N. (2013): Dynamics of yield components and stevioside production in Stevia rebaudiana grown under different planting times, plant stands and harvest regime. Ind. Crop. Prod., 50, 731736.

    • Search Google Scholar
    • Export Citation
  • Singla, R. & Jaitak, V. (2016): Synthesis of rebaudioside A from stevioside and their interaction model with hTAS2R4 bitter taste receptor. Phytochemistry, 125, 106111.

    • Search Google Scholar
    • Export Citation
  • Vaněk, T., Nepovim, A. & Valiček, P. (2001): Determination of stevioside in plant material and fruit teas. J. Food Compos. Anal., 14, 383388.

    • Search Google Scholar
    • Export Citation
  • Zhang, S.Q., Kumar, A. & Kutowy, O. (2000): Membrane-based separation scheme for processing sweeteners from stevia leaves. Food Res. Int., 33, 617620.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 26 63
Jul 2020 0 27 37
Aug 2020 0 21 58
Sep 2020 0 14 40
Oct 2020 0 27 72
Nov 2020 0 16 44
Dec 2020 0 0 0