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  • a Center of Biotechnology of BorjCedria, BP 901, 2050, Hammam-Lif, Tunisia
  • b University of Tunis El Manar, Campus University, Tunis 1060, Tunisia
  • c CREA Research Centre for Olive, Citrus and Tree Fruit, C. da Li Rocchi, 87036 Rende (CS), Italy
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The aim of the present study was to investigate the biological activities of Tunisian olive leaf extracts and to correlate these activities to their phytochemical composition. The phenolic profile of four Tunisian autochthonous cultivars Chemlali, Sayali, Neb jmel, and Meski was determined using LC/MS-MS. The antioxidant activity of olive leaf extracts was evaluated using DPPH test. The antiproliferative effect was also investigated using MTT assay. The phytochemical screening showed that phenolic content and phenolic class repartition were significantly affected by olive leaf cultivars. Twenty-one components were identified and oleurpein, luteolin 4-glucoside, luteolin 7-glucoside, and apigenin 7-glucoside were the major phenolic components. Among all extracts, Sayali exhibited the strongest antioxidant and antiproliferative activities (IC50 41.36 µg ml−1 / EC50 147.11 µg ml−1). The MTT result showed that olive leaf extract reduced MCF-7 cell viability in a dose-dependent manner. Western blot analysis demonstrated that olive leaf extracts exhibited antiproliferative activity through apoptosis induction.

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L. Abrankó (Budapest), J. Baranyi (Norwich), I. Bata-Vidács (Budapest), J. Beczner (Budapest), Gy. Biró (Budapest), A. Blázovics (Budapest), F. Capozzi (Bologna), M. Carcea (Rome), Zs. Cserhalmi (Budapest), M. Dalla Rosa (Bologna), I. Dalmadi (Budapest), K. Demnerova (Prague), Muying Du (Chongqing), S. N. El (Izmir), S. B. Engelsen (Copenhagen), E. Gelencsér (Budapest), V. M. Gómez-López (Murcia), J. Hardi (Osijek), N. Ilić (Novi Sad), D. Knorr (Berlin), H. Köksel (Ankara), R. Lásztity (Budapest), K. Liburdi (Cork), M. Lindhauer (Detmold), M.-T. Liong (Penang), M. Manley (Stellenbosch), M. Mézes (Gödöllő), Á. Németh (Budapest), Q. D. Nguyen (Budapest), L. Nyström (Zürich), V. Piironen (Helsinki), A. Pino (Catania), M. Rychtera (Prague), K. Scherf (München), R. Schönlechner (Wien), A. Sharma (Mumbai), A. Szarka (Budapest), M. Szeitzné Szabó (Budapest), L. Varga (Mosonmagyaróvár), R. Venskutonis (Kaunas), B. Wróblewska (Olsztyn)

 

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