View More View Less
  • 1 The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland
Restricted access

The aim of this study was to determine the effect of selected factors on rye (Secale cereale L.) haploid embryo production by the wide crossing method. The study was performed on fifteen winter rye genotypes. This is the first time for rye when besides the genotype, on the enlargement of ovaries and haploid embryo production, such factors as: type of auxin analogues 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropyridine-2-carboxylic acid (picloram), and the time between florets emasculation and pollination were investigated. All factors had a significant impact on rye ovary enlargement, however the haploid embryo formation depended only on rye genotype, not on kind of auxin and days between emasculation to pollination. In total, twenty one haploid embryos were formed by six genotypes of fifteen tested. On average, 13.86% (after 2,4-D treatment) to 20.05% (after dicamba treatment) enlarged ovaries per emasculated florets were obtained. Most of the ovaries enlarged when florets were pollinated 4 and 6 days after emasculation. Most of the haploid embryos formed when florets were pollinated 6 days after emasculation. The obtained haploid embryos did not germinate.

  • Altpeter, F., Korzun, V. 2007. Rye. In: Pua, E.C., Davey, M.R. (eds) Biotechnology in Agriculture and Forestry, Transgenic Crops IV, vol. 59 Springer-Verlag, Berlin Heidelberg, pp. 107117.

    • Search Google Scholar
    • Export Citation
  • Altenhofer, P., Oertel, C., Matzk, F. 1997. Chromosome elimination in wide crosses of Poaceae. Current topics in plant cytogenetics related to plant improvement. International Symposium held at Tulln, Austria, February 21–22, pp. 310317.

    • Search Google Scholar
    • Export Citation
  • Deimling, S., Flehinghaus-Roux, T., Rober, F., Schechert, A., Roux, S.R., Geiger, H.H. 1994. Doubled haploid production-now reproducible in rye. In: Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, June 12–17, p. 95.

    • Search Google Scholar
    • Export Citation
  • Deimling, S., Flehinghaus-Roux, T. 1997. Haploidy in rye. In: Jain, M.S., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Kluwer Academic Publishers, Dordrecht, pp. 181204.

    • Search Google Scholar
    • Export Citation
  • Flehinghaus, T., Deimling, S., Geiger, H.H. 1991. Methodical improvements in rye anther culture. Plant Cell Rep. 10:397400.

  • Flehinghaus-Roux, T., Deimling, S., Geiger, H.H. 1995. Anther culture ability in Secale cereale L. Plant Breed. 114:259261.

  • Forster, B.P., Heberle-Bors, E., Kasha, K.J., Touraev, A. 2007. The resurgence of haploids in higher plants. Trends Plant Sci. 12:368375.

    • Search Google Scholar
    • Export Citation
  • Guo, Y.D., Pulli, S. 2000. Isolated microspore culture and plant regeneration in rye (Secale cereale L.). Plant Cell Rep. 19:875880.

  • Hoagland, D.R., Arnon, D.I. 1938. A water culture method for growing plants without soil. Circ. Univ. Calif., Agric. Exp. Stn., No. 347.

    • Search Google Scholar
    • Export Citation
  • Hörlein, A.J. 1991. Metodische Untersuchungen zur Antherenkultur bei Roggen. Metodische Untersuchungen zur Antherenkultur bei Roggen. Dissertation zur Erlagung des Grades eines Doktors der Agrarwissenschaften, Universität Hohenheim, Stuttgart.

    • Search Google Scholar
    • Export Citation
  • Hromada-Judycka, A., Bolibok-Bragoszewska, H., Rakoczy-Trojanowska, M. 2010. Genetically directed differential subtraction chain products related to in vitro response of immature embryos of rye (Secale cereale L.): isolation, characterization, and expression analysis Plant Cell Tiss. Org. Cult. 100:131138.

    • Search Google Scholar
    • Export Citation
  • Immonen, S. 1999. Androgenetic green plants from winter rye, Secale cereale L., of diverse origin. Plant Breeding 118:319322.

  • Immonen, S., Anttila, H. 1996. Success in anther culture of tye. Proc. EUCARPIA Int. Symp. Rye Breeding & Genetics. Vortr. Pflanzenziichtg. 35:237244.

    • Search Google Scholar
    • Export Citation
  • Immonen, S., Anttila, H. 1999. Cold pretreatment to enhance green plant regeneration from rye anther culture. Plant Cell Tiss. Org. Cult. 57:121127.

    • Search Google Scholar
    • Export Citation
  • Immonen, S., Anttila, H. 2000. Media composition and anther plating for production of androgenetic green plants from cultivated rye (Secale cereale L.). J. Plant Physiol. 156:204210.

    • Search Google Scholar
    • Export Citation
  • Karimi-Ashtiyani, R., Ishii, T., Niessen, M., Stein, N., Heckmann, S., Gurushidze, M. 2015. Point mutation impairs centromeric CENH3 loading and induces haploid plants. Proc. Natl. Acad. Sci. U.S.A. 112:1121111216.

    • Search Google Scholar
    • Export Citation
  • Laurie, D.A., O’Donoughue, LS., Bennett, M.D. 1990. Wheat × maize and other wide sexual hybrids: their potential for genetic manipulation and crop improvement. In: Gustafson, J.P. (ed.), Genetic manipulation in plant improvement II, Plenum Press, New York, pp. 95126.

    • Search Google Scholar
    • Export Citation
  • Ma, R., Guo, Y., Pulli, S. 2004. Comparison of anther and microspore culture in the embryogenesis and regeneration of rye. Plant Cell Tiss. Org. Cult. 76:147157.

    • Search Google Scholar
    • Export Citation
  • Mikolajczyk, S., Broda, Z., Weight, D. 2012. The effect of cold temperature stress on the viability of rye (Secale cereale L.) microspores. J. Biotech. 93(2):139142.

    • Search Google Scholar
    • Export Citation
  • Noga A. , Skrzypek, E., Warchol, M., Czyczylo-Mysza, I., Dziurka, K., Marcinska, I., Juzon, K., Warzecha, T., Sutkowska, A., Nita, Z., Werwinska, K. 2016. Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media. In Vitro Cellular & Developmental Biology –Plant 52:590597.

    • Search Google Scholar
    • Export Citation
  • Ponitka, A., Slusarkiewicz-Jarzina, A. 2004. Cleared-ovule technique used for rapid access to elary embryo development in Secale cereale x Zea mays crosses. Acta Biol. Cracov. Series Botanica 46:133137.

    • Search Google Scholar
    • Export Citation
  • Rakoczy-Trojanowska, M., Smiech, M., Malepszy, S. 1997. The influence of genotype and medium on rye (Secale cereale L.) anther culture. Plant Cell Tiss. Org. Cult. 48:1521.

    • Search Google Scholar
    • Export Citation
  • Rubtsova, M., Gnad, H., Melzer, M., Weyen, J., Gils, M. 2013. The auxins centrophenoxine and 2,4-D differ in their effects on non-directly induced chromosome doubling in anther culture of wheat (T. aestivum L.) Plant Biotechnol. Rep. 7:247255.

    • Search Google Scholar
    • Export Citation
  • Rybczynski, J.J. 1990. Plant tissue culture of Secale: A review. Euphytica 46:5770.

  • Sserumaga, J.P., Oikeh, S.O., Mugo, S., Asea, G., Otim, M., Beyene, Y., Abalo, G., Kikafunda, J. 2015. Genotype by environment interactions and agronomic performance of doubled haploids testcross maize (Zea mays L.) hybrids. Euphytica 207:353365.

    • Search Google Scholar
    • Export Citation
  • Tenhola-Roininen, T., Immonen, S., Tanhuanpää, P. 2006. Rye doubled haploids as a research and breeding tool –a practical point of view. Plant Breed. 125:584590.

    • Search Google Scholar
    • Export Citation
  • Targonska, M., Hromada-Judycka, A., Bolibok-Bragoszewska, H., Rakoczy-Trojanowska, M. 2013. The specificity and genetic background of the rye (Secale cereale L.) tissue culture response. Plant Cell. Rep. 32(1):19.

    • Search Google Scholar
    • Export Citation
  • Thomas, E., Wenzel, G. 1975. Embryogenesis from microspores of rye. Naturwissenschaften 62:4041.

  • Wenzel, G., Thomas, E. 1974. Observations on the growth in culture of anthers of Secale cereale. Z. Pflanzenzüchtg. 72:8994.

  • Wenzel, G., Hoffmann, E., Thomas, E. 1977. Increased induction and chromosome doubling of androgenetic haploid rye. Theor. Appl. Genet. 5:8186.

    • Search Google Scholar
    • Export Citation
  • Zenkteler, M., Misiura, E. 1974. Induction of androgenic embryos from cultured anthers of Hordeum, Secale, and Festuca. Biochem. Physiol. Pflanzen. 165:337340.

    • Search Google Scholar
    • Export Citation
  • Zenkteler, M., Nitzsche, W. 1984. Wide hybridization experiment in cereals. Theor. Appl. Genet. 68:311315.

  • Zhuang, J.J., Xu, J. 1983. Increasing differentiation frequencies in wheat pollen callus. In: Hu, H., Vega, M.R. (eds) Cell and Tissue Culture Techniques for Cereal Crop Improvement, Science Press, Beijing, p. 431.

    • Search Google Scholar
    • Export Citation

Click HERE for submission guidelines

Manuscript submission: CRC Manuscript Submission

 

Senior editors

Editor(s)-in-Chief: Pauk, János

Technical Editor(s): Hajdu Buza, Kornélia

Technical Editor(s): Lantos, Csaba

Editorial Board

  • A. Aniol (Poland)
  • P. S. Baenziger (USA)
  • R.K. Behl (India)
  • F. Békés (Australia)
  • L. Bona (Hungary)
  • A. Börner (Germany)
  • R. N. Chibbar (Canada)
  • S. Gottwald (Germany)
  • A. Goyal (Canada)
  • H. Grausgruber (Austria)
  • T. Harangozó (Hungary)
  • E. Kapusi (Austria)
  • E.K. Khlestkina (Russia)
  • J. Kolmer (USA)
  • V. Korzun (Germany)
  • R. A. McIntosh (Australia)
  • Á. Mesterházy (Hungary)
  • A. Mohan (USA)
  • I. Molnár (Hungary)
  • M. Molnár-Láng (Hungary)
  • A. Pécsváradi (Hungary)
  • S. K. Rasmussen (Denmark)
  • N. Rostoks (Latvia)
  • M. Taylor (Germany)
  • J. Zhang (China)
  • X.F. Zhang (USA)

 

Senior Editorial Board

  • P. Bartos (Czech Republic)
  • H. Bürstmayr (Austria)
  • J. Johnson (USA)
  • Z. Kertész (Hungary)
  • G. Kimber (USA)
  • J. Matuz (Hungary)

Cereal Research Communications
Cereal Research Non-Profit Ltd. Company
Address: P.O. Box 391, H-6701 Szeged, Hungary
Phone: +36 62 435 235
Fax: +36 62 420 101
E-mail: crc@gk-szeged.hu

Indexing and Abstracting Services:

  • AgBiotechNet Abstracts
  • Agricola
  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Current Contents/Agriculture
  • Biology & Environmental Sciences
  • ISI Web of Science/li>
  • Science Citation Index Expanded
  • SCOPUS

 

Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)