values and mobile phase composition have been determined for the six antihyperlipidemic agents-bezafibrate, ciprofibrate, clofibrate, clofibric acid, fenofibrate, and gemfibrozil. The drugs were separated on silica gel, CN, and Diol plates using mobile phases containing
-hexane as weakly polar diluent and five polar modifiers: acetone, dioxane, ethyl methyl ketone, ethyl acetate, and tetrahydrofuran. The optimum mobile phases were also investigated on alumina, NH
, and polyamide phases for comparison. The linearity of relationships between
and modifier volume fraction, molar fraction, and logarithm of molar fraction was calculated. Plates were developed in horizontal chambers, visualized under UV irradiation at
= 254 nm, and scanned with a densitometer in the multi-wavelength scan mode. Most results fitted the
> 0.9; for approximately half
> 0.99. Separation of all the drugs was achieved on Diol plates with mobile phases containing 20–30% of each modifier in
-hexane, and with hexane-acetone, 9 + 1, on CN plates. Five drugs were separated using the same mobile phases on silica gel. The best separation was obtained on Diol plates with tetrahydrofuran-hexane, 2 + 8, as mobile phase.
E.M. Assem de Juarez
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