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  • 1 Jagiellonian University Department of Pharmacognosy, Collegium Medicum Medyczna 9 30-688 Cracow Poland
  • | 2 Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum Medyczna 9 30-688 Cracow Poland
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Conditions have been established for determination of diosmin in pharmaceutical formulations, for example ‘Diosminin’, by TLC with densitometric detection. Chromatographic separation on silica gel was performed with chloroform-methanol-water, 23 + 12 + 2 ( v/v ), as mobile phase and the spots were analyzed by densitometry at λ = 344 nm. Under the experimental conditions established repeatable and accurate results were obtained. The method is characterized by high sensitivity — the limit of detection was 20 ng — and satisfactory recovery — from 99.8 to 103.0%. Response was linearly dependent on concentration in the range 5 to 50 μg mL −1 .

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