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  • 1 University of Skopje Faculty of Pharmacy Vodnjanska St 17 Skopje R. Macedonia
  • 2 Faculty of Technology and Metallurgy Rudjer Boskovic St 16 Skopje R. Macedonia
  • 3 Health Protection Agency Food Control Department Skopje R. Macedonia
  • 4 Institute of Chemistry Faculty of Science Arhimedova St Skopje R. Macedonia
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A quantitative high-performance thin-layer chromatographic (HPTLC) method for analysis of gallic acid and tannin in extracts of Arctostaphylos uva-ursi (L.) Sprengel, bearberry leaves (Ericaceae), and validation of the method, are described. Analyses were performed on cellulose F 254 HPTLC plates with iso -butanol-acetic acid-water, 14 + 1 + 3.5 ( v/v ), as mobile phase. The chromatographic system afforded satisfactory RF (0.35 for gallotannin and 0.46 for gallic acid), low RSD (0.1–0.44%), and effective resolution between components ( RS = 1.89). The uva-ursi leaves examined contained approximately 14% tannin and 5% gallic acid. Special features of the thin-layer chromatographic (TLC) technique were taken into consideration in the validation process and calculation of validation data for the method. This HPTLC method was found to be simple, reliable, and convenient for routine analysis. Its analytical performance fulfilled acceptance criteria established for TLC methods in the official literature.

  • C.A. Newall, L.A. Anderson , and J.D. Phillipson , Herbal Medicines: A Guide for Health-Care Professionals, The Pharmaceutical Press, London, 1996.

    Phillipson J.D. , '', in Herbal Medicines: A Guide for Health-Care Professionals , (1996 ) -.

  • M. Wichtl and N.G. Bisset (Eds), Herbal Drugs and Phytopharmaceuticals, Medpharm Scientific Publishers, Stuttgart, 1994.

    '', in Herbal Drugs and Phytopharmaceuticals , (1994 ) -.

  • ESCOP “ Uvae ursi folium”, Monographs on the Medicinal Uses of Plant Drugs, European Scientific Cooperative on Phytotherapy, Exeter, UK, 1997.

  • D. Frohne , Planta Med. 18 (1970) 1–25.

    Frohne D. , '' (1970 ) 18 Planta Med. : 1 -25.

  • C. Wahner, J. Schonert , and H. Friedrich , Pharmazie 29 (1974) 616–617

    Friedrich H. , '' (1974 ) 29 Pharmazie : 616 -617.

  • O Blake, R Booth , and D. Corrigan , Br. J. Phytother. 3 (1994) 124–127.

    Corrigan D. , '' (1994 ) 3 Br. J. Phytother. : 124 -127.

  • E. Halsam , Plant Polyphenols, Vegetable Tannins Revised, Cambridge University Press, 1989.

  • I. Asenov and S. Nikolov , Farmakognozia, Medicina i Fzikultura, Sofija, 1988.

  • K.H. Inoue and A.E. Hagerman , Anal. Biochem. 169 (1988) 363–369.

    Hagerman A.E. , '' (1988 ) 169 Anal. Biochem. : 363 -369.

  • J.B. Harborne , Phytochemical Methods: A Guide to Modern Techniques in Plant Analysis, 2nd edn, Chapman and Hall, London, 1984.

    Harborne J.B. , '', in Phytochemical Methods: A Guide to Modern Techniques in Plant Analysis , (1984 ) -.

  • J.B. Harborne , Methods in Plant Biochemistry, Vol. 1, Plant Phenolics, Academic Press, London, 1989.

    Harborne J.B. , '', in Methods in Plant Biochemistry, Vol. 1, Plant Phenolics , (1989 ) -.

  • M. Bálint , Gyógyszerészet 28 (5) (1984) 177–181.

    Bálint M. , '' (1984 ) 28 Gyógyszerészet : 177 -181.

  • General Chapter 1225, Validation of Compendial Methods, United States Pharmacopeia XXII National Formulary XVII, The United States Pharmacopoeia Convention, Rockville, MD, 1990, pp. 1612–1710.

    '', in Validation of Compendial Methods, United States Pharmacopeia XXII National Formulary XVII , (1990 ) -.

  • K. Ferenczi-Fodor, Z. Végh , and Z. Pap-Sziklay , J. Planar Chromatogr. 6 (1993) 198–203.

    Pap-Sziklay Z. , '' (1993 ) 6 J. Planar Chromatogr. : 198 -203.

  • P.R. Bradley , British Compendium, Vol. 1, British Herbal Medicine Association, 1992.

  • G.A. Ivanova and Y.V. Vikhareva , Farmatsiya Moskow 48 (6) (1999) 35–36.

    Vikhareva Y.V. , '' (1999 ) 48 Farmatsiya Moskow : 35 -36.

  • V. Shultz, R. Hansel , and V. E. Tyler , Rational Phytotherapy, A Physicans’ Guide to Herbal Medicine, Springer, Berlin, 1997.

    Tyler V. E. , '', in Rational Phytotherapy, A Physicans’ Guide to Herbal Medicine , (1997 ) -.

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