The retention behavior of amino acids on cellulose layers has been determined. With organic-aqueous mobile phases, linear second order relationships were established between volume fraction of organic solvent (tetrahydrofuran, acetonitrile, and 2-propanol) in water and retention,
, for all the solutes investigated. The results obtained experimentally were compared with those calculated by use of polynomial and
models of retention. Multiple development techniques (UMD and IMD) were also used. Predicted and experimentally obtained results were compared and assessed statistically. Chromatographic systems with the best separation selectivity were used for analysis of amino acids in reference solutions used for testing blood plasma.