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  • 1 Skubiszewski Medical University Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland
  • 2 Skubiszewski Medical University Department of Physical Chemistry Staszica 6 20-081 Lublin Poland
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The retention factor RF is used in several criteria generally known as chromatographic response functions (CRF). In TLC and HPTLC most of these are based on differences between the retention factors of two substances, which are summed or multiplied. There are also other functions, for example the multispot response function ( MRF ) which enables the quality of a separation to be estimated. Although good CRF criteria should have well-defined distribution and range, current criteria based on the differences do not satisfy this requirement. Only MRF has a clearly defined range (0 to 1), but its distribution is unstable. In this paper two new independent coefficients: RU (retention uniformity) and RD (retention distance) are proposed; these always have values within the range 0 to 1 and stable density, irrespective of the number of compounds separated. Their reliable mathematical properties have been tested in wide range by Monte-Carlo simulations. An example is given of their use in the separation of fibrate-type antihyperlipidemic drugs by normal and reversed-phase TLC (114 systems).

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