A ternary mobile phase for thin-layer chromatographic separation and identification of seven amino acids on microcrystalline cellulose, with ninhydrin as detection reagent, has been optimized by use of the experimental design software packages
. When the results from each product were compared the optimum mobile phase was found to be butanol-glacial acetic acid-water 60.14:18.77:21.09. The performance of this mobile phase was confirmed experimentally.
, Thin-Layer Chromatography, Springer, New York, 1969.
Stahl E. , '', in Thin-Layer Chromatography, (1969) -.