An HPTLC method, using an internal standard, for analysis of colchicine in a pharmaceutical formulation, has been established and validated. The analyte and internal standard were separated on aluminum plates precoated with silica gel 60 F
; the mobile phase was ethyl acetate-acetonitrile-water-formic acid 8.0:1.0:0.5:0.5 (
). Quantification was by densitometric scanning at 358 nm. Response was a linear function of colchicine concentration in the range 5 to 35 μg mL
. The limits of detection and quantification for colchicine were 1 and 5 μg mL
, respectively. Average recovery of colchicine was 100.48%, which showed the method was free from interference from excipients present in the formulation. The established method enabled accurate, precise, and rapid analysis of colchicine in the pharmaceutical formulation.
The Merck Index, 14th edn, monograph 2470 for colchicine, p. 414.
(ed.), Martindale, The Complete Drug Reference, 33rd edn, 2002, p. 400.