Two new simple and accurate methods for determination of quetiapine in tablets-normal phase (NP) and reversed-phase (RP) high performance thin layer chromatography (HPTLC), each with densitometric and videodensitometric detection, were developed and validated. NP-HPTLC was developed with HPTLC silica F
plates and hexane-dioxane-propylamine 1:9:0.4 (
) as mobile phase. RPHPTLC was carried out using HPTLC RP8 F
plates with tetrahydrofuran-phosphate buffer, pH 9.0, 5:5 (
) as mobile phase. The silica gel plates were developed to a distance of 9 cm and RP8 plates to a distance of 4.5 cm. Both analyses were performed in horizontal chambers and scanned with a densitometer at 243 nm and a video-densitometer at 254 nm. Calibration plots were linear in the range 0.2–1.2 μg quetiapine per spot for NP-HPTLC and in the range 0.1–1.1 μg for RP-HPTLC. The precision and accuracy of the four methods were fully compared and no significant differences were observed. The methods can be used in routine pharmaceutical analysis.
O. Gefvert, M. Bergstrom, B. Langstrom, T. Lundberg, L. Lindstrom, R. Yates