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  • 1 Russian Academy of Sciences Laboratory of Medical and Biological Research, Department of Biologically Active Substances, Institute of General and Experimental Biology, Siberian Division Sakh’yanovoy St. 6 670047 Ulan-Ude Russian Federation
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A thin-layer chromatographic method has been established for quantification of kurarinone and sophoraflavanone G in the roots of Sophora flavescens Soland. (Fabaceae). Chromatographic separation was performed on silica gel HPTLC plates with chloroform-methanol 10:1 (ν/ν) as mobile phase. The plates were scanned densitometrically at 285 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise — intra-day and inter-day RSD were 1.97–2.04% and 2.15–2.40%, respectively. Instrument precision and repeatability of the method were 0.43–0.56 and 1.81–1.84 (CV [%]), respectively. Accuracy was checked by measuring recovery at four levels; average recovery was 96.3–103.4% for kurarinone and 96.8–102.9% for sophoraflavanone G. The method was used for analysis of kurarinone and sophoraflavanone G in Sophora flavescens roots and was confirmed to be suitable for this purpose.

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