Russian Academy of Sciences Laboratory of Medical and Biological Research, Department of Biologically Active Substances, Institute of General and Experimental Biology, Siberian Division Sakh’yanovoy St. 6 670047 Ulan-Ude Russian Federation
A thin-layer chromatographic method has been established for quantification of kurarinone and sophoraflavanone G in the roots of Sophora flavescens Soland. (Fabaceae). Chromatographic separation was performed on silica gel HPTLC plates with chloroform-methanol 10:1 (ν/ν) as mobile phase. The plates were scanned densitometrically at 285 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise — intra-day and inter-day RSD were 1.97–2.04% and 2.15–2.40%, respectively. Instrument precision and repeatability of the method were 0.43–0.56 and 1.81–1.84 (CV [%]), respectively. Accuracy was checked by measuring recovery at four levels; average recovery was 96.3–103.4% for kurarinone and 96.8–102.9% for sophoraflavanone G. The method was used for analysis of kurarinone and sophoraflavanone G in Sophora flavescens roots and was confirmed to be suitable for this purpose.
Bh.V. Dush, Materia Medica of Tibetan Medicine, Sri Satguru Publications, Delhi, 1994.
Dush Bh.V., '', in Materia Medica of Tibetan Medicine, (1994) -.
Dush Bh.V.Materia Medica of Tibetan Medicine1994)| false
X.-L. Piao, X.-Sh. Pia, S.W. Kim, J.H. Park, H.Y. Kim, Sh.-Q. Cai, Biol. Pharm. Bull. 29 (2006) 1911–1915.
Cai Sh.-Q., '' (2006) 29Biol. Pharm. Bull.: 1911-1915.
Cai Sh.-Q.Biol. Pharm. Bull.20062919111915)| false
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