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  • 1 La Trobe University School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences Edwards Rd. Bendigo 3550 Australia
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There is a large chemical variability between commercially available Echinacea for both research and consumption impacts on the quality and efficacy of these products. Globally, herbal industries are largely unregulated, leaving manufacturers responsible for quality control. Several recent efforts to improve regulation have created a need for rapid, accurate, and reliable assays suitable for a quality control setting. Thus, the aim of this study was to develop and validate a high-performance thin-layer chromatographic (HPTLC) method for the quantitative analysis of commercial Echinacea products. HPTLC and high-resolution plate imaging were used to separate and quantify the amounts of three phenylpropanoid compounds which were used then as markers in product quality assessment. The developed method was validated according to the current International Conference on Harmonization (ICH) guidelines in terms of accuracy, precision, linearity, specificity, limits of quantification and detection, and robustness and was used to quantify these markers in three commercial Echinacea formulations. The method achieved better limits of detection than previous assays, despite exhibiting lower repeatability, particularly when quantifying cichoric acid. This work has demonstrated that high-resolution plate imaging can be used as a reliable method for the quantitative analysis of commercial Echinacea products.

  • S. Foster, Chapt. 3 Medicinal Uses of Echinacea by Eclectic Physicians, in: Echinacea: Nature’s Immune Enhancer, Healing Arts Press, Vermont, 1990, pp. 35–38.

    Foster S. , '', in Echinacea: Nature’s Immune Enhancer , (1990 ) -.

  • S.E. Binns, J. Hudson, S. Merali, J.T. Arnason, Planta Med. 68 (2002) 780–783.

    Arnason J.T. , '' (2002 ) 68 Planta Med. : 780 -783.

  • A. Chicca, F. Pellati, B. Adinolfi, A. Matthias, I. Massarelli, S. Benvenuti, E. Martinotti, A.M. Bianucci, K. Bone, R. Lehmann, P. Nieri, Br. J. Pharmacol. 153 (2008) 879–885.

    Nieri P. , '' (2008 ) 153 Br. J. Pharmacol. : 879 -885.

  • A. Cheminat, R. Zawatzky, H. Becker, R. Brouillard, Phytochemistry 27 (1988) 2787–2794.

    Brouillard R. , '' (1988 ) 27 Phytochemistry : 2787 -2794.

  • B. Classen, S. Thude, W. Blaschek, M. Wack, C. Bodinet, Phytomedicine 13 (2006) 688–694.

    Bodinet C. , '' (2006 ) 13 Phytomedicine : 688 -694.

  • C.A. LaLone, K.D. Hammer, L. Wu, J. Bae, N. Leyva, Y. Liu, A.K. Solco, G.A. Kraus, P.A. Murphy, E.S. Wurtele, O.K. Kim, K.I. Seo, M.P. Widrlechner, D.F. Birt, J. Agric. Food Chem. 55 (2007) 7314–7322.

    Birt D.F. , '' (2007 ) 55 J. Agric. Food Chem. : 7314 -7322.

  • S.E. Binns, B. Purgina, C. Bergeron, M.L. Smith, L. Ball, B.R. Baum, J.T. Arnason, Planta Med. 66 (2000) 241–244.

    Arnason J.T. , '' (2000 ) 66 Planta Med. : 241 -244.

  • Z. Zhai, Y. Liu, L. Wu, D.S. Senchina, E.S. Wurtele, P.A. Murphy, M.L. Kohut, J.E. Cunnick, J. Med. Food 10 (2007) 423–434.

    Cunnick J.E. , '' (2007 ) 10 J. Med. Food : 423 -434.

  • Z. Zhai, D. Haney, L. Wu, A. Solco, P.A. Murphy, E.S. Wurtele, M.L. Kohut, J.E. Cunnick, Food Agric. Immunol. 18 (2007) 221–236.

    Cunnick J.E. , '' (2007 ) 18 Food Agric. Immunol. : 221 -236.

  • K. Linde, B. Barrett, K. Wolkart, R. Bauer, D. Melchart, Cochrane Database Syst. Rev. (2006) CD000530.

    Melchart D. , '', in Cochrane Database Syst. Rev. , (2006 ) -.

  • J.B. Calixto, Braz. J. Med. Biol. Res. 33 (2000) 179–189.

    Calixto J.B. , '' (2000 ) 33 Braz. J. Med. Biol. Res. : 179 -189.

  • P. Molgaard, S. Johnsen, P. Christensen, C. Cornett, J. Agric. Food Chem. 51 (2003) 6922–6933.

    Cornett C. , '' (2003 ) 51 J. Agric. Food Chem. : 6922 -6933.

  • W. Li, Y. Sun, R.B. Van Breeman, J.F. Fitzloff, J. Liq. Chromatogr. Relat. Technol. 27 (2004) 2507–2529.

    Fitzloff J.F. , '' (2004 ) 27 J. Liq. Chromatogr. Relat. Technol. : 2507 -2529.

  • J. Lee, J. Funct. Foods 2 (2010) 158–162.

    Lee J. , '' (2010 ) 2 J. Funct. Foods : 158 -162.

  • W. Luo, C.Y. Ang, T.A. Gehring, T.M. Heinze, L.J. Lin, A. Mattia, J. AOAC Int. 86 (2003) 202–208.

    Mattia A. , '' (2003 ) 86 J. AOAC Int. : 202 -208.

  • J. Lee, C.F. Scagel, J. Funct. Foods 2 (2010) 77–84.

    Scagel C.F. , '' (2010 ) 2 J. Funct. Foods : 77 -84.

  • J. Barnes, L.A. Anderson, S. Gibbons, J.D. Phillipson, J. Pharm. Pharmacol. 57 (2005) 929–954.

    Phillipson J.D. , '' (2005 ) 57 J. Pharm. Pharmacol. : 929 -954.

  • N.B. Perry, J.W. van Klink, E.J. Burgess, G.A. Parmenter, Planta Med. 63 (1997) 58–62.

    Parmenter G.A. , '' (1997 ) 63 Planta Med. : 58 -62.

  • S. Bent, J. Gen. Intern. Med. 23 (2008) 854–859.

    Bent S. , '' (2008 ) 23 J. Gen. Intern. Med. : 854 -859.

  • M.M. Srivastava, High-Performance Thin-Layer Chromatography (HPTLC), Springer, Heidelberg, 2011.

    Srivastava M.M. , '', in High-Performance Thin-Layer Chromatography (HPTLC) , (2011 ) -.

  • N. Sahoo, P. Manchikanti, S. Dey, Fitoterapia 81 (2010) 462–471.

    Dey S. , '' (2010 ) 81 Fitoterapia : 462 -471.

  • S. Sudberg, E.M. Sudberg, J. Terrazas, K. Patel, J. Pineda, B. Fine, J. AOAC Int. 93 (2010) 1367–1375.

    Fine B. , '' (2010 ) 93 J. AOAC Int. : 1367 -1375.

  • Y.Z. Liang, P. Xie, K. Chan, J. Chromatogr. B 812 (2004) 53–70.

    Chan K. , '' (2004 ) 812 J. Chromatogr. B : 53 -70.

  • A. Schibli, E. Reich, J. Planar Chromatogr. 18 (2005) 34–38.

    Reich E. , '' (2005 ) 18 J. Planar Chromatogr. : 34 -38.

  • R.B. Wills, K. Bone, M. Morgan, Nutr. Res. Rev. 13 (2000) 47–77.

    Morgan M. , '' (2000 ) 13 Nutr. Res. Rev. : 47 -77.

  • C.M. Gilroy, J.F. Steiner, T. Byers, H. Shapiro, W. Georgian, Arch. Int. Med. 163 (2003) 699–704.

    Georgian W. , '' (2003 ) 163 Arch. Int. Med. : 699 -704.

  • M.F.M. Trypsteen, R.G.E. Van Severen, B.M.J. De Spiegeleer, Analyst 114 (1989) 1021–1024.

    Spiegeleer B.M.J. , '' (1989 ) 114 Analyst : 1021 -1024.

  • S. Gocan, G. Cimpan, L. Muresan, J. Pharmaceut. Biomed. Anal. 14 (1996) 1221–1227.

    Muresan L. , '' (1996 ) 14 J. Pharmaceut. Biomed. Anal. : 1221 -1227.

  • R. Bauer, P. Remiger, Planta Med. 55 (1989) 367–371.

    Remiger P. , '' (1989 ) 55 Planta Med. : 367 -371.

  • R. Bauer, I.A. Khan, H. Wagner, Planta Med. 54 (1988) 426–430.

    Wagner H. , '' (1988 ) 54 Planta Med. : 426 -430.

  • E. Reich, A. Blatter, R. Jorns, M. Kreuter, K. Thiekötter, J. Planar Chromatogr. 15 (2002) 244–251.

    Thiekötter K. , '' (2002 ) 15 J. Planar Chromatogr. : 244 -251.

  • W. Georgian, J. Planar Chromatogr. 15 (2002) 71–73.

    Georgian W. , '' (2002 ) 15 J. Planar Chromatogr. : 71 -73.

  • T. Tie-xin, W. Hong, J. Chromatogr. Sci. 46 (2008) 560–564.

    Hong W. , '' (2008 ) 46 J. Chromatogr. Sci. : 560 -564.

  • J. Sherma, Anal. Chem. 82 (2010) 4895–4910.

    Sherma J. , '' (2010 ) 82 Anal. Chem. : 4895 -4910.

  • C.F. Poole, J. Chromatogr. A 1000 (2003) 963–984.

    Poole C.F. , '' (2003 ) 1000 J. Chromatogr. A : 963 -984.

  • B. Spangenberg, C.F. Poole, C. Weins, Chapt. 9, Planar Chromatography Detectors, in: Quantitative Thin-Layer Chromatography: A Practical Survey, Springer, Heidelberg, 2010, pp. 231–260.

    Weins C. , '', in Quantitative Thin-Layer Chromatography: A Practical Survey , (2010 ) -.

  • R. Johnsson, G. Traff, M. Sunden, U. Ellervik, J. Chromatogr. A 1164 (2007) 298–305.

    Ellervik U. , '' (2007 ) 1164 J. Chromatogr. A : 298 -305.

  • R.E. Simon, L.K. Walton, Y. Liang, M.B. Denton, Analyst 126 (2001) 446–450.

    Denton M.B. , '' (2001 ) 126 Analyst : 446 -450.

  • A.V. Gerasimov, J. Anal. Chem. 59 (2004) 348–353.

    Gerasimov A.V. , '' (2004 ) 59 J. Anal. Chem. : 348 -353.

  • C.F. Poole, J. Chromatogr. A 856 (1999) 399–427.

    Poole C.F. , '' (1999 ) 856 J. Chromatogr. A : 399 -427.

  • J. Sherma, Anal. Chem. 74 (2002) 2653–2662.

    Sherma J. , '' (2002 ) 74 Anal. Chem. : 2653 -2662.

  • G.W. Schieffer, J. Nutraceut. Nutr. 3 (2000) 67–81.

    Schieffer G.W. , '' (2000 ) 3 J. Nutraceut. Nutr. : 67 -81.

  • M. Petrovic, M. Kastelan-Macan, D. Ivankovic, S. Matecic, J. AOAC Int. 83 (2000) 1457–1462.

    Matecic S. , '' (2000 ) 83 J. AOAC Int. : 1457 -1462.

  • R. Johnsson, G. Träff, M. Sundén, U. Ellervik, J. Chromatogr. A 1164 (2007) 298–305.

    Ellervik U. , '' (2007 ) 1164 J. Chromatogr. A : 298 -305.

  • B. Spangenberg, C.F. Poole, C. Weins, Chapt. 4, The Mobile Phase in Adsorption and Partition Chromatography, in: Quantitative Thin-Layer Chromatography, Springer, Heidelberg, 2011, pp. 81–104.

    Weins C. , '', in Quantitative Thin-Layer Chromatography , (2011 ) -.

  • B. Nüsslein, M. Kurzmann, R. Bauer, W. Kreis, J. Nat. Prod. 63 (2000) 1615–1618.

    Kreis W. , '' (2000 ) 63 J. Nat. Prod. : 1615 -1618.

  • I.E.W. Group, Validation of Analytical Procedures: Text and Methodology Q2 (R1), in: International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Geneva, 2005.

    '', in International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Geneva , (2005 ) -.

  • S. Ebel, J. Planar Chromatogr. 9 (1996) 4–15.

    Ebel S. , '' (1996 ) 9 J. Planar Chromatogr. : 4 -15.

  • D.L. Massart, B.G.M. Vandeginste, S.N. Deming, Y. Michotte, L. Kaufman, Chapt. 2, Precision and Accuracy; Chapt. 7, Sensitivity and Limits of Detection; Chapt. 8, Selectivity and Specificity, in: Chemometrics: A Textbook, Elsevier, Amsterdam, 1988.

    Kaufman L. , '', in Chemometrics: A Textbook , (1988 ) -.

  • H.T. Karnes, C. March, Pharmaceut. Res. 10 (1993) 1420–1426.

    March C. , '' (1993 ) 10 Pharmaceut. Res. : 1420 -1426.

  • M. Prosek, A. Golc-Wondra, T. Maver, M. Fir, J. Planar Chromatogr. 17 (2004) 102–108.

    Fir M. , '' (2004 ) 17 J. Planar Chromatogr. : 102 -108.

  • J.K. Taylor, Anal. Chem. 53 (1981) 1588A–1596A.

    Taylor J.K. , '' (1981 ) 53 Anal. Chem. : 1588A -1596A.

  • CAMAG, TLC Visualiser: The Documentation and Evaluation System with High-Resolution 12 bit CCD camera, CAMAG, Muttenz, 2003.

    '', in TLC Visualiser: The Documentation and Evaluation System with High-Resolution 12 bit CCD camera , (2003 ) -.

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