View More View Less
  • 1 CSIR ‒ National Botanical Research Institute, Lucknow (U.P.) 226001, India
Restricted access

A validated online over-pressured layer chromatography (OPLC) method was developed for the rapid, cost-effective, and efficient separation of gallic acid. A reversed-phase (RP) ultraviolet (UV)–online OPLC analytical method was developed and validated as per the International Conference on Harmonization (ICH) guidelines. Methanolic extract of Annona muricata (fruit) was prepared by cold maceration. Separation of marker was achieved on RP-OPLC plates (5 × 20 cm) with isocratic solvent system of 0.1% acetic acid, water and methanol (30:70) at a flow rate of 0.15 mL min−1; detection was carried out at λmax 270 nm. The base peak of standard gallic acid was at 5.441 min with good linearity (r2 > 0.999), precision, and accuracy. The limit of detection (LOD) (521.84 mg mL−1) and limit of quantification (LOQ) (1581.336 mg mL−1) values reflect that the method is sensitive with high peak purity; the recoveries of analyte were 99 to 103%. The achievement of the method was the early retention time of gallic acid which in turn increased the efficiency of the quantification of the targeted marker in a short duration of time for even larger number of samples in plant extract as well as biological fluids for pharmacokinetic studies. The application of the method can be extended in regulatory guidelines for the quality control of herbal drugs/products and formulations. The method is rapid and economical in terms of solvent consumption and, hence, can be preferred over other high-performance thin-layer chromatographic or liquid chromatographic methods.

  • [1]

    M. Karamac , A. Kosinska, R.B. Pegg, Polish J. Food Nutri. Sci. 15 (2006) 5558.

  • [2]

    I. Kubo , N. Masuoka, T. Joung, K. Shimizu, K. Nihei, Open Bioact. Compd. J. 3 (2010) 111.

  • [3]

    Z. Sun , L. Zhao, L. Zuo, C. Qi, P. Zhao, X. Hou, J. Chromatogr. B 858 (2014) 5562.

  • [4]

    F. Maa , X. Gong, X. Zhou, Y. Zhao, M. Li, J. Ethnopharmacol. 162 (2015) 377383.

  • [5]

    W. Phakthong , B. Liawruangrath, S. Liawruangrath, Talanta 130 (2014) 577584.

  • [6]

    E. Tyihak , E. Mincsovics, A.M. Moricz, J. Chromatogr. A 1232 (2012) 318.

  • [7]

    C.J.M. Cardozo , V.V. Lozano, D.P.Y. Betancur, H.J.C. Velasquez, J.R.C. Valenzuela, Revista Facultad Nacional de Agronomi Medellin 65 (2012) 64776486.

    • Search Google Scholar
    • Export Citation
  • [8]

    V.C. George , D.R.N. Kumar, V. Rajkumar, P.K. Suresh, R.A. Kumar, Asian Pacific J. Cancer Prevent. 13 (2011) 699704.

  • [9]

    D.K. Singh , S.K. Srivastava, A.K.S. Rawat, Acta Chromatogr. 19 (2007) 246252.

  • [10]

    N.A. Santagati , L. Salerno, G. Attaguile, F. Savoca, G. Ronsisvalle, J. Chromatogr. Sci. 46 (2008) 150156.

  • [11]

    A. Zhang , L. Wan, C. Wu, Y. Fang, G. Han, H. Li, Z. Zhang, H. Wang, Molecules 18 (2013) 1424114257.

  • [12]

    ICH, Q2R1 Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization, Geneva, 2005.

  • [13]

    P. Oroszlan , G. Verzar-Petri, E. Mincsovics, T. Székely, A. Vastagh, Proc. Intern. Symposium on TLC with Special Emphasis on OPLC (Szeged, 1984), Labor MIM, Budapest (E. Tyihák ed.), 1986, pp. 343352.

    • Search Google Scholar
    • Export Citation
  • [14]

    M. Hevesi , A.M. Moricz, Z.K. Veghely, M. Toth, Gy. Katay, E. Tyihak, J. Planar Chromatogr. 23 (2010) 411414.

  • [15]

    A.M. Moricz , S. Szarka, P.G. Ott, E.B. Hethelyi, E. Szoke, E. Tyihak, Med. Chem. 8 (2012) 8594.

  • [16]

    S. Srivastava , A. Misra, P.K. Shukla, B. Kumar, S. Lata, A.K.S. Rawat, RSC Adv. 4 (2014) 6090260906.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2020 3 5 1
Dec 2020 1 0 0
Jan 2021 0 0 0
Feb 2021 0 0 0
Mar 2021 1 0 0
Apr 2021 0 0 0
May 2021 0 0 0