View More View Less
  • 1 KLE University, Nehru Nagar, Belagavi, Karnataka 590 010, India
Restricted access

Two different botanical sources, Eclipta alba and Wedelia calendulacea are used as “Bhringaraja” in the Ayurvedic s ystem o f medicine. In the present study, an effort has been made to evaluate different sources by using high-performance thin-layer chromatography (HPTLC) as an analytical tool. Wedelolactone, one of the primary constituents of these plants, was taken as the marker compound for the evaluation. An HPTLC method was developed and validated for the evaluation of different sources of “Bhringaraja”. The chromatographic system was developed using silica gel 60 F254 HPTLC plates with the mobile phase toluene–chloroform–ethyl alcohol–formic acid (5:4:1:0.5, v/v). Linearity was found between the concentration ranges of 80 to 280 ng spot−1 (R2: 0.9994), and limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.36 ng spot−1 and 1.09 ng spot−1. The study has shown the presence of wedelolactone at the concentration of 0.26% w/w and 0.05% w/w in E. alba and W. calendulacea, respectively, whereas it was absent in another closely related species, Wedelia trilobata. At the same time, all the three plants were subjected to evaluation of quality-control parameters as per the World Health Organization (WHO) guidelines. Certain parameters such as foaming index, total alkaloids, and total bitter principles were significantly different in the three plants. Hence, the present HPTLC method development, and the validation and evaluation of quality-control parameters would be helpful in the standardization of individual plants and their formulations.

  • [1]

    K. K. Hullatti , M. S. Sharada, Indian J. Pharm. Sci. 72 (2010) 3945.

  • [2]

    http://www.techno-preneur.net/technology/project-profiles/food/bhring. html.

  • [3]

    B. Vaidya , Some Controversial Drugs in Indian Medicine, Oriental Publication, New Delhi, 1982.

  • [4]

    Anonymous, The Ayurvedic Pharmacopoeia of India, Part I, Vol. II, Ministry of Health and Family Welfare, Department of Ayush Govt. of India, New Delhi, 2008.

  • [5]

    P. V. Neeraja , E. Margaret, IJCPR 2 (2012) 18897.

  • [6]

    W. T. Shiera , A. C. Shiera, W. Xieb, C. J. Mirochab, Toxicon 39 (2001) 14351438.

  • [7]

    H. S. Oh , R. C. Smart, Proc. Natl. Acad. Sci. USA 93 (1996) 1252512530.

  • [8]

    S. M. Wong , S. Antus, A. Gottsegen, B. Fessler, G. S. Rao, J. Sonnenbichler, H. Wagner, Arzneim. Forsch. 38 (1988) 661665.

  • [9]

    I. Nomani , A. Mazumder, G. S. Chakraborthy, Int. J. PharmTech Res. 5 (2013) 95764.

  • [10]

    C. P. Khare (ed.) , Indian Medicinal Plants, Springer-Verlag, Berlin/Heidelberg, 2007.

  • [11]

    N. Balekar , T. Nakpheng, T. Srichana, Chiang Mai J. Sci. 41 (2014) 590605.

  • [12]

    Y. Liu , L. Zhan, T. Liu, M. Cheng, X. Liu, J. Ethnopharmacol. 157 (2014) 206211.

  • [13]

    Y. Xia , J. Chen, Y. Cao, C. Xu, R. Li, Y. Pan, Eur. J. Pharmacol. 714 (2013) 105111.

  • [14]

    D. Manvar , M. Mishra, S. Kumar, V. N. Pandey, J. Ethnopharmacol. 144 (2012) 545554.

  • [15]

    P. Cos , A. J. Vlietinck, D. V. Berghe, L. Maes, J. Ethnopharmacol. 106 (2006) 290302.

  • [16]

    J. Bruneton , Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd edn., Lavoisier Publishing Inc., Secaucus, NJ, 1999.

  • [17]

    Anonymous, Quality Control Methods for Medicinal Plants Materials, WHO, Geneva, 2011.

  • [18]

    P. Rashmin , P. Mrunali, D. Nitin, D. Nidhi, P. Bharat, J. Food Drug Anal. 20 (2012) 794804.

  • [19]

    G. A. Shabir , J. Chromatogr. A 987 (2003) 5766.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 2 0 0
Jul 2021 3 0 0
Aug 2021 10 0 0
Sep 2021 4 0 0
Oct 2021 11 0 0
Nov 2021 2 0 0
Dec 2021 0 0 0