View More View Less
  • 1 M. Curie-Skłodowska University, 3, M. Curie-Skłodowska Square, 20-031 Lublin, Poland
  • 2 University of Life Sciences, 13, Akademicka Street, 20-950 Lublin, Poland
  • 3 University of Pécs, 2, Rókus Street, 7624 Pécs, Hungary
  • 4 Hungarian Academy of Sciences, 15, Herman Ottó Street, 1022 Budapest, Hungary
Open access

In this study, thin-layer chromatography—direct bioautography (TLC—DB) was used for guiding the isolation and identification of antibacterial constituents of Thymus vulgaris L. ethanol extract. This TLC—bioassay method enables the separation and detection of active components directly on the surface of chromatographic plates. They can be identified by comparison with reference substances or using physicochemical methods, preferably spectroscopic ones (liquid chromatography—tandem mass spectrometry [LC—MS/MS], in the presented paper). The described method belongs to the effect-directed analyses (EDA). Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, including methicillin-resistant Staphylococcus aureus as well as luminescent bacteria like Aliivibrio fischeri. Five fractions with the widest antimicrobial spectra were detected using TLC—DB, isolated by semi-preparative TLC and subjected to LC—MS/MS analyses. Finally, two bioactive components were tentatively identified, basing on their fragmentation pattern, as eriodictyol and 4,4′-dihydroxy-5,5′-diisopropyl-2,2′-dimethyl-3,6-bifenylodion.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1]

    W. C. Evans , Pharmacognosy, Saunders, London, 2000.

  • [2]

    M. Soleimani , A. P. Daryasari, A. Ghorbani, O. M. Hejri, R. Mazaheri, J. Essent. Oil Bear. Pl. 17 (2014) 1233-{in1240}.

  • [3]

    I. M. Choma , W. Jesionek, Instrumental Thin-Layer Chromatography: Effects-Directed Biological Detection: Bioautography, Elsevier, Amsterdam, 2014.

    • Search Google Scholar
    • Export Citation
  • [4]

    G. E. Morlock , W. Schwack, J. Chromatogr. A 1217 (2010) 6600-6609.

  • [5]

    I. M. Choma , E. M. Grzelak, J. Chromatogr. A 1218 (2011) 2684-2691.

  • [6]

    A. Marston , J. Chromatogr. A 1218 (2011) 2676-2683.

  • [7]

    W. Jesionek , E. M. Grzelak, B. Majer-Dziedzic, I. M. Choma, J. Planar Chromatogr. 26 (2013) 109-113.

  • [8]

    W. Jesionek , Á. M. Móricz, Á. Alberti, P. G. Ott, B. Kocsis, G. Horváth, I. M. Choma, J. AOAC Int. 98 (2015) 1013-1020.

  • [9]

    Á. M. Móricz , E. Fornal, W. Jesionek, B. Majer-Dziedzic, I. M. Choma, Chromatographia 78 (2015) 707716.

  • [10]

    Á. M. Móricz , P. G. Ott, A. Böszörményi, E. Lemberkovics, E. Mincsovics, E. Tyihák, Chromatographia 75 (2012) 991999.

  • [11]

    G. Horváth , L. Szabó, É. Héthelyi, É. Lemberkovics, J. Essent. Oil Res. 18 (2006) 315-317.

  • [12]

    N. Nakatani , K. Miura, T. Inagaki, Agric. Biol. Chem. 53 (1989) 1375-1381.

  • [13]

    N. Fabre , I. Rustan, E. Hoffmann, J. Quetin-Leclercq, J. Am. Soc. Mass Spectrom. 12 (2001) 707-715.

  • [14]

    H. Wagner , S. Bladt, Plant Drug Analysis: A Thin Layer Chromatography Atlas, 2nd edn., Springer, Munich, 2001.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 5 0
Jul 2020 0 5 6
Aug 2020 0 1 5
Sep 2020 0 5 25
Oct 2020 0 6 10
Nov 2020 0 3 6
Dec 2020 0 0 0