A simple, cost-effective, precise, accurate, and rapid planar chromatographic method was developed and validated for the separation and determination of amodiaquine and artesunate in tablet formulations. Both compounds were determined using high-performance thin-layer chromatography plates and a mobile phase composed of toluene, acetonitrile, methanol, ammonium acetate, and triethylamine in the ratio 10:5:3:1:0.5 (% v/v). Amodiaquine was evaluated densitometrically at a detection wavelength of λ = 345 nm, whereas artesunate was determined fluorimetrically at λ = 503 nm. The method was linear in the concentration ranges of 0.093–0.280 μg spot−1 for artesunate and 0.250–1.250 μg spot−1 for amodiaquine, respectively.
R.S.Vardanyan, V.J.Hruby, Synthesis of Essential Drugs, Elsevier Science, Amsterdam, 2006.