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  • 1 Tanzania Food and Drug Authority, Dar es Salaam, Tanzania
  • 2 University of Würzburg, Am Hubland, 97074 Würzburg, Germany
  • 3 Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
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A simple, cost-effective, precise, accurate, and rapid planar chromatographic method was developed and validated for the separation and determination of amodiaquine and artesunate in tablet formulations. Both compounds were determined using high-performance thin-layer chromatography plates and a mobile phase composed of toluene, acetonitrile, methanol, ammonium acetate, and triethylamine in the ratio 10:5:3:1:0.5 (% v/v). Amodiaquine was evaluated densitometrically at a detection wavelength of λ = 345 nm, whereas artesunate was determined fluorimetrically at λ = 503 nm. The method was linear in the concentration ranges of 0.093–0.280 μg spot−1 for artesunate and 0.250–1.250 μg spot−1 for amodiaquine, respectively.

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