Among the complex mixture of biologically active compounds in Leptadenia pyrotechnica, three compounds have been used as analytical markers. A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for the estimation. Methanolic extracts of whole plants from three populations were used on aluminum pre-coated silica gel 60 F254 plates with different mobile phases to determine the amount of β-sitosterol, lupeol, and oleanolic acid with RF value of 0.64, 0.84, and 0.47, respectively. The calibration curve was linear in the range of 2–10 μg. The method is reliable for the quantification, separation, and good resolution of these compounds from other constituents of L. pyrotechnica. To ascertain the purity of the peak from the test sample, its in-situ reflectance spectrum was compared with that from standards; the clear superimposability indicated the purity of the peaks.
N.R.Shaw, G.K.Singh, Int. J. Pharm. Biol. Sci.5 (2014) 671–675.
N.R.Shaw, G.K.Singh, Int. J. Pharm. Biol. Sci.5 (2014) 671–675.)| false
Validation of analytical procedure: methodology Q2 (R1), International Conference on Harmonization (ICH), International Federation of Pharmaceutical Manufacturers and Associations (IFPMA),Geneva, 1996.
P.D.Hamrapurkar, P.Karishma, J. Planar Chromatogr.20 (2007) 183–187.
P.D.Hamrapurkar, P.Karishma, J. Planar Chromatogr.20 (2007) 183–187.)| false