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  • 1 Dalhousie University Trace Analysis Research Centre, Department of Chemistry B3H 4J1 Halifax N. S. (Canada)
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Concentrations of up to 16 elements have been determined in subcellular fractions of bovine kidney using INAA methods. Levels of Rb and Se have been measured by a cyclic INAA method. A conventional INAA method consisting of 2 irradiations and 3 counting periods has been employed to determine the other elements. Accuracy of measurements has been evaluated by analyzing several standard reference materials. Trace element content of reagents used has been investigated in detail. Fresh samples of bovine kidney have been homogenized in a buffer containing sucrose and HEPES, and the homogeneate separated into nuclei, mitochondrial, lysosomal, microsomal and cytosol fractions by successive differential centrifugation. Concentrations of trace elements have been measured in these fractions using the INAA methods.