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  • 1 Comenius University Department of Analytical Chemistry, Faculty of Natural Sciences 842 15 Bratislava Slovak Republic
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Two fractions of both fulvic acids (FA) and humic acid (HA) were prepared by fractionation method of Pierce and Felbeck5 involving acid hydrolysis of soil rests. This step increases recovery of both FA and HA considerably what suggest us need for slight modification of IHSS method in some cases. The weight loss, change in organic carbon content and visible spectra are figures of merit discussed. After detailed characterization these humic substances (HS) will serve as the working standards for study of interactions between organomercurials and organic part of soil.