In the study of aluminum toxicity for plant, it is important to analyzethe behavior of Al in cells. We present how Al is taken up by tobacco cellsthrough lumogallion staining method developed by our laboratory. The fluorescenceintensity from the cell was increased rapidly between 4 and 8 hours of 1 mMAl treatment. To calibrate the fluorescence intensity from Al-lumogallioncomplex, Al amount in the cell was determined by NAA. When the same samplewas analyzed by ICP-AES, Al amounts in all the samples were 13% lower thanthose measured by NAA.