A normal-phase high-performance liquid-chromatographic method has been developed for simultaneous quantitative analysis of phenmedipham, desmedipham, and ethofumesate in a pesticide formulation. Analysis was performed on a 25 cm × 0.4 cm, 5-μm particle, CN column with n-hexane-dichloromethane 40:60 (v/v) as mobile phase at a flow rate of 1 mL min–1. UV detection was performed at 270 nm; the constant column temperature was 25°C. The run time under these chromatographic conditions was less than 8 min. Calibration plots were linear in the concentration range 76–380 μg mL–1 for phenmedipham, 72–360 μg mL–1 for desmedipham, and 52–260 μg mL–1 for ethofumesate. Statistical evaluation by analysis of variance showed the intra-day repeatability (n = 8) and inter-day precision (n = 3) of the assay were satisfactory. The sensitivity of the method, as the limits of detection (LOD) and quantification (LOQ) for each active ingredient, was also determined.
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