Authors:
T. Sivakumar Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

Search for other papers by T. Sivakumar in
Current site
Google Scholar
PubMed
Close
,
R. Manavalan Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

Search for other papers by R. Manavalan in
Current site
Google Scholar
PubMed
Close
, and
K. Valliappan Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

Search for other papers by K. Valliappan in
Current site
Google Scholar
PubMed
Close
Open access

Summary

This paper deals with optimization of a liquid-liquid extraction procedure for simultaneous HPLC analysis of domperidone and pantoprazole in human plasma. Central composite design and Derringer's desirability function were used to optimize the concentration of KOH and the volume of ethyl acetate as the main factors affecting the liquid-liquid extraction procedure. After extraction, the analytes were separated quantitatively on a C18 column with 10 mM pH 7.0 phosphate buffer-methanol-acetonitrile 48.46:20:31.54 (υ/υ) as mobile phase at a flow rate of 1.20 mL min−1 and with UV detection at 285 nm. It was concluded that extraction recovery of both the analytes was affected by KOH concentration and that recovery of pantoprazole was affected by ethyl acetate (extraction solvent) volume. Extraction recovery under optimum extraction conditions was 93.52% for domperidone and 92.72% for pantoprazole. The optimized extraction method was validated. Linearity was established for six levels in the ranges 10–1000 ng mL−1 for pantoprazole and 15–1000 ng mL−1 for domperidone. The lower limit of quantitation (LLOQ) and detection (LOD) were estimated as 9.84 and 5.91 ng mL−1, respectively, for pantoprazole and 14.56 and 8.79 ng mL−1 for domperidone. The optimized method was linear, specific, accurate, and precise; the high recovery (>92%) and low relative standard deviation (<2.5%) enable reliable quantification of these analytes in spiked human plasma.

  • [1]. A. Fitton L. Wiseman 1995 Drugs 51 460.

  • [2]. J.A. Barone 1999 Ann. Pharmacother. 33 429.

  • [3]. R. Huber W. Muller M.C. Banks S.J. Rogers P.C. Norwood E. Doyle 1990 J. Chromatogr. 529 389.

  • [4]. N.V.S. Ramakrishna K.N. Vishwottam S. Wishu M. Koteshwara 2005 J. Chromatogr. B 822 326.

  • [5]. M. Tanaka H. Yamazaki 1996 Anal, Chem. 68 1513.

  • [6]. Q.B. Cass A.L.G. Degani N.M. Cassiano J. Pedrazolli Jr 2001 J. Chromatogr. B 766 153.

  • [7]. O. Peres C.H. Oliveira A.R.E. Barrientos V.M. Rezende G. de -Nucci 2004 Arzneimittelforschung 54 314.

  • [8]. I. Heykants R. Hendriks W. Meuldermans M. Michiels H. Scheygrond H. Reyntjens 1981 Eur. J. Drug Metab. Pharmacokinet. 6 61.

  • [9]. Y.C. Huang J.L. Colaizzi R.H. Bierman R. Woestenborghs J.J. Heykants 1986 J. Clin. Pharmacol. 26 628.

  • [10]. W. Meuldermans R. Hurkmans E. Swysen J. Hendricks M. Michiels W. Lauwers J. Heykants 1981 Eur. J. Drug Metab. Pharmacokinet. 6 49.

    • Search Google Scholar
    • Export Citation
  • [11]. M. Michiels R. Hendriks J. Heykants 1981 Eur. J. Drug Metab. Pharmacokinet. 6 37.

  • [12]. K. Yamamoto M. Hagino H. Kotaki T. Iga 1998 J. Chromatogr. B 720 251.

  • [13]. M. Kobylinska K. Kobylinska 2000 J. Chromatogr. B 744 207.

  • [14]. A.P. Zavitsanos C. MacDonald E. Bassoob D. Gopaul 1999 J. Chromatogr. B 730 9.

  • [15]. M.J. Smit F.C.W. Sutherland H.K.L. Hundt K.J. Swart A.F. Hundt J. Els 2002 J. Chromatogr. A 949 65.

  • [16]. M.J. Sáiz-Abajo J.M. González-Sáiz C. Pizarro 2005 Anal. Chim. Acta 528 63.

  • [17]. T. Sivakumar R. Manavalan K. Valliappan 2007 Acta Chromatogr. 19 29.

  • [18]. T. Sivakumar R. Manavalan C. Muralidharan K. Valliappan 2007 J. Sep. Sci. 30 3143.

  • [19]. T. Sivakumar R. Manavalan C. Muralidharan K. Valliappan 2007 J. Pharm. Biomed. Anal. 43 1842.

  • [20]. FDA-Center for Drug Evaluation and Research (CDER): Guidance for industry, Bioanalytical method validation, 2001.

  • [21]. S. Mitra 2003 Sample Preparation Techniques in Analytical Chemistry John Wiley and Sons Hoboken, New Jersey 37138.

  • [22]. A. Jadoul V. Préat 1997 Int. J. Pharm. 154 229.

  • [23]. K. Florey 2002 Analytical Profiles of Drug Substances and Excipients Elsevier Science New York 213259.

  • [24]. T. Lundstedt E. Seifert L. Abramo B. Thelin Nyström J. Pettersen R. Bergman 1998 Chemom. Intell. Lab. Syst. 42 3.

  • [25]. Q. Beg V. Sahai R. Gupta 2003 Process Biochem. 39 203.

  • [26]. G. Derringer R. Suich 1980 J. Qual. Technol. 12 214.

  • [27]. P. Wester J. Gottfries K. Johansson F. Klintebäck B. Winblad 1987 J. Chromatogr. 415 261.

  • Collapse
  • Expand

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • SCOPUS
  • DOAJ
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2024  
Scopus  
CiteScore  
CiteScore rank  
SNIP  
Scimago  
SJR index 0.285
SJR Q rank Q3

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 700 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads