Authors:
M. Szaufer-Hajdrych Department of Pharmacognosy, Poznań University of Medical Sciences, Święcickiego 4, 61-771, Poznań, Poland

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W. Bylka Department of Pharmacognosy, Poznań University of Medical Sciences, Święcickiego 4, 61-771, Poznań, Poland

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I. MatłAwska Department of Pharmacognosy, Poznań University of Medical Sciences, Święcickiego 4, 61-771, Poznań, Poland

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M. Wójciak-Kosior Department of Chemistry, Medical University of Lublin, Staszica 6, 20-081, Lublin, Poland

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G. Matysik Department of Chemistry, Medical University of Lublin, Staszica 6, 20-081, Lublin, Poland

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J. Jodynis-Liebert Department of Toxicology, Poznań University of Medical Sciences, Dojazd 30, 60-631, Poznań, Poland

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Summary

Densitometric HPTLC and HPLC have been used for quantification of p-coumaric and protocatechuic acids in an ethereal fraction from a methanolic extract of Aquilegia vulgaris L. HPLC analysis was performed on an RP-18 column with methanol-water-formic acid 25:75:0.5 (υ/υ) as mobile phase. Thin layer chromatography was performed on Si60 F254 HPTLC plates with mixtures of heptane, dichloromethane, diisopropyl ether, formic acid, and water as mobile phases. Satisfactory separation of the phenolic acids was achieved by use of the multiple gradient development technique. The quantities of p-coumaric and protocatechuic acids determined by HPLC were 0.374 and 2.283 mg g−1 dry plant material, respectively; HPTLC results were somewhat higher — 0.396 and 2.584 mg g−1, respectively. The precision of both methods, expressed as relative standard deviation, was satisfactory. The methods are useful for quality control of Aquilegia vulgaris extracts.

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Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

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2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
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Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
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SJR Q rank Q3

Acta Chromatographica
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Acta Chromatographica
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
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Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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