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  • 1 Chair and Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090, Lublin, Poland
  • 2 Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland
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Summary

A new objective chromatographic response function, RK, based on the kernel density estimate, is introduced for estimation of the fingerprinting performance of a particular TLC system (uniformity of retention) for which a large set of experimental RF values of possible components of the mixture is available. The RK criterion is insensitive to large numbers (hundreds or thousands) of RF values, when the previously proposed criteria cease. It can be applied to one and two-dimensional TLC and is easily computed. As an example of its application, the performance of twelve general screening systems was evaluated in the context of herbal extract fingerprinting (88 phytochemical standards) by both one and two-dimensional TLC.

  • [1]. E.L. Inman H.J. Tenbarge 1988 J. Chromatogr. Sci. 26 89.

  • [2]. L. Pasti B. Walczak D.L. Massart P. Reschiglian 1999 Chemom. Intell. Lab. Syst. 48 21.

  • [3]. B. Walczak W. Wu 2005 Chemom. Intell. Lab. Syst. 77 173.

  • [4]. V. Pravdova B. Walczak D.L. Massart 2002 Anal. Chim. Acta 456 77.

  • [5]. A.M. van Nederkassel M. Daszykowski P.H.C. Eilers Y. Vander Heyden 2006 J. Chromatogr. A 1118 199.

  • [6]. M. Daszykowski B. Walczak 2006 Trends Anal. Chem. 25 1081.

  • [7]. M. Waksmundzka-Hajnos J. Sherma T. Kowalska 2008 Thin Layer Chromatography in Phytochemistry CRC Press Francis and Taylor, Boca Raton, USA.

    • Search Google Scholar
    • Export Citation
  • [8]. P. Owen A. Pendlebury A.C. Moffat 1978 J. Chromatogr. 161 187.

  • [9]. P. Owen A. Pendlebury A.C. Moffat 1978 J. Chromatogr. 161 195.

  • [10]. A.C. Moffat 1975 J. Chromatogr. 110 341.

  • [11]. G. Musumarra G. Scarlatta G. Cirma G. Romano S. Palazzo S. Clementi G. Giuletti 1984 J. Chromatogr. 295 31.

  • [12]. G. Musumarra G. Scarlatta G. Cirma G. Romano S. Palazzo S. Clementi G. Giuletti 1985 J. Chromatogr. 350 151.

  • [13]. A.C. Moffat 2004 Clarke's Analysis of Drugs and Poisons (3rd edn) Pharmaceutical Press London.

  • [14]. Komsta W. Markowski G. Misztal 2007 J. Planar Chromatogr. 20 27.

  • [15]. Komsta 2008 Acta Chromatogr. 20 309.

  • [16]. D.W. Scott 1992 Multivariate Density Estimation. Theory, Practice and Visualization Wiley New York.

  • [17]. R Development Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org.

    • Search Google Scholar
    • Export Citation