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  • 1 Chair and Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090, Lublin, Poland
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Summary

The thin-layer chromatographic (TLC) retention of 35 model compounds has been investigated with ten screening mobile phases on six normal-phase and seven reversed-phase adsorbents. The retention factors formed two cubes with dimensions 35 × 10 × 6 and 35 × 10 × 7, respectively, which enabled three-way analysis by PARAFAC. A one-component PARAFAC model was optimum in both cases and two-component models performed worse. The one-component model explained 78.8% of the variance in normal-phase chromatography and 94.2% of the variance in reversed-phase chromatography. These results showed that the major variability of the retention factor (RF) can be modelled as the product of three factors related to the substance itself, the mobile phase, and the adsorbent. RF modelling was substantially better than using k or RM (rate mobility) values.

  • [1]. I. Baranowska S. Świerczek 1995 J. Planar Chromatogr. 8 357.

  • [2]. B. Klama T. Kowalska 1996 J. Planar Chromatogr. 9 25.

  • [3]. M. Waksmundzka-Hajnos 1996 Chromatographia 43 640.

  • [4]. M. Waksmundzka-Hajnos B. Wrońska 1996 Chromatographia 43 405.

  • [5]. M. Waksmundzka-Hajnos A. Hawrył 1998 J. Planar Chromatogr. 11 283.

  • [6]. M. Waksmundzka-Hajnos A. Petruczynik E. Soczewiński A. Hawrył 2002 Chem. Anal. (Warsaw) 47 483.

  • [7]. A. Petruczynik M. Waksmundzka-Hajnos A. Hawrył A. Ziółkowska 2003 Chem. Anal. (Warsaw) 48 881.

  • [8]. W. Zapała K. Kaczmarski T. Kowalska 2002 J. Chromatogr. Sci. 40 575.

  • [9]. W. Zapała M. Waksmundzka-Hajnos 2004 J. Liq. Chromatogr. 27 2127.

  • [10]. R. Bro 1997 Chemom. Intell. Lab. Syst. 38 149.

  • [11]. C.A. Andersson R. Bro 2000 Chemom. Intell. Lab. Syst. 52 1.

  • [12]. Komsta 2007 Anal. Chim. Acta 593 224.

  • [13]. R.W. Kennard L.A. Stone 1969 Technometrics 11 137.

  • [14]. E. Soczewiński J. Jusiak 1981 Chromatographia 14 23.