Acta Chromatographica
Volume/Issue:
Volume 22: Issue 2
Characterization of oligoglucan-containing products derived from Mesorhizobium loti HAMBI 1148 murein after lysozyme digestion and β-elimination
Authors:
M. Karaś Department of Genetics and Microbiology, Maria Curie-Skłodowska University, 20-033, Lublin, Poland

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and
R. Russa Department of Genetics and Microbiology, Maria Curie-Skłodowska University, 20-033, Lublin, Poland

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Pages:
323–335
Online Publication Date:
01 Jun 2010
Publication Date:
01 Jun 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.22.2010.2.13
Keywords:
peptidoglycan; rhizobium; glucan oligomer; β-elimination
Open access

Summary

Short glucan oligomers were found to be covalently linked to the peptidoglycan fragments liberated by partial lysozyme digestion of Mesorhizobium loti HAMBI 1148 murein and among some muropeptide mono and oligomers subsequently subjected to alkaline β-elimination. The mass spectra of the preparation before and after treatment with alkali contained deprotonated ions [M-H]− recorded at m/z 3107 and 2856 which represented muropeptide fragments with 2 or 3 glucose residues, respectively, at the reducing end. Because of the lack of a free reducing group, muramyl residues remained untouched during the β-elimination reaction in a manner comparable with muropeptide fragments protected by 1,6-anhydromuramic acid represented in the spectra by [M-H]− ions with masses of 2451 and 2762 m/z. The ion [M-H]− at m/z 1615 which was proposed to represent a disaccharide fragment with one Glc residue linked had no counterpart in the spectra of lysozyme-liberated muropeptides and was supposed to have arisen from a dimer (ions [M + H]+ 2023 m/z and [M-H]− 2021 m/z observed for muropeptides not treated with alkali) after elimination of a GlcNAc-acetamidohexenopyranose fragment in the alkaline environment. According to a similar mechanism, the ion at m/z 2723 [M-H]− determined as a hexasaccharide fragment with an anhydromuramic acid residue at the end arose from a dimer represented by ion [M-H]− with mass 3128 m/z. The data presented suggest that glycan strands in rhizobium murein can be protected at the reducing end by short oligoglucans except for 1,6-anhydromuramic acid residues.

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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
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Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
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ISSN 2083-5736 (Online)

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