Acta Chromatographica
Volume/Issue:
Volume 22: Issue 3
RP-HPLC analysis of withanolides in the flowers, leaves, and roots of Withania somnifera
Authors:
N. W. Ali Faculty of Pharmacy, University of Beni-Sueif, Beni-Sueif, Egypt

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S. Abouzid Faculty of Pharmacy, University of Beni-Sueif, Beni-Sueif, Egypt

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A. Nasib H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

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S. Khan H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

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J. Qureshi H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

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M. I. Choudhary H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

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Pages:
473–480
Online Publication Date:
01 Sep 2010
Publication Date:
01 Sep 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.22.2010.3.10
Keywords:
Solanaceae; RP-HPLC; withanolides; withaferin A; flower
Open access

Summary

Ashwaghanda, Withania somnifera, is one of the most widely used herbs in Ayurvedic medicine. Leaves and roots are the traditionally used parts of the plant. An RP-HPLC method using a binary acetonitrile-water gradient containing 0.1% acetic acid has been developed for analysis of withaferin A. The method was validated in accordance with ICH guidelines and used for analysis of the withanolide content of the flowers, leaves, and roots of W. somnifera. The withanolide content was highest in the flowers.

  • [1]. R. Schliebs S.K. Liebmann A. Kumar S. Ghosal V. Bigl 1973 Neurochem. Int. 30 181.

  • [2]. A.B. Ray M. Gupta 1994 Prog. Chem. Org. Nat. Prod. 63 2.

  • [3]. S.K. Bhattacharya K.S. Satyan S. Ghosal 1997 Indian J. Exp. Biol. 35 236.

  • [4]. S.K. Kulkarni B. George R. Mathur 1998 Phytother. Res. 12 451.

  • [5]. J.N. Dhuley 2001 Phytother. Res. 15 311.

  • [6]. A.D. Kinghorn B.N. Su D.S. Jang L.C. Chang D. Lee J.Q. Gu E.J. Carcache-Blanco A.C. Pawlus S.K. Lee E.J. Park M. Cuedei J.J. Gills K. Bhat H.S. Park E. Mata-Greenwod L.L. Song M. Jang J.M. Pessuto 2004 Planta Med. 70 691.

    • Search Google Scholar
    • Export Citation

  • [7]. C. Tohda K. Komaatuse T. Kuboyama 2005 J. Tradit. Med. 22 176.

  • [8]. H. Ichikawa Y. Takada S. Shishodia B. Jayaprakasam M.G. Nair B.B. Aggrawal 2006 Mol. Cancer Ther. 5 1434.

  • [9]. M. Kaileh W.V. Berghe A. Heyerick J. Horion J. Plette C. Libert D. De Keukelerie T. Essawi G. Haegeman 2007 J. Biol. Chem. 282 4253.

    • Search Google Scholar
    • Export Citation

  • [10]. R.S. Sangwan , N.D. Chaurasiya, L.N. Misra, P. Lal, G.C. Uniyal, N.S. Sangwan, A.K. Srivastava, K.A. Suri, G.N. Qazi, and R. Tuli, US Patent # 7, 108, 870 (2005).

  • [11]. N.D. Chaurasiya G.C. Uniyal P. Lal L. Misra N.S. Sangwan R. Tuli R.S. Sangwa 2008 Phytochem. Anal. 19 148.

  • [12]. S. Dalavayi S.M. Kulkarni R.L. Itikala S. Itikala 2006 Ind. J. Pharm. Sci. 68 253.

  • [13]. M. Ganzera M.I. Choudhary I.A. Khan 2003 Fitoterapia 74 68.

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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
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CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
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SJR Q rank Q3

Acta Chromatographica
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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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