Quantification of negundoside in Vitex negundo Linn. Leaf powder by high-performance liquid chromatography

P. J. Lokhande; J. K. Verma

doi:10.1556/achrom.22.2010.4.8

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Acta Chromatographica

Volume/Issue:: Volume 22: Issue 4

Quantification of negundoside in Vitex negundo Linn. Leaf powder by high-performance liquid chromatography

Authors:

P. J. Lokhande

P. J. Lokhande Inorganic Chemistry Laboratory, K.J. Somaiya College of Science and Commerce, Vidyavihar, Mumbai, 400 077, Maharashtra, India

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J. K. Verma

J. K. Verma Inorganic Chemistry Laboratory, K.J. Somaiya College of Science and Commerce, Vidyavihar, Mumbai, 400 077, Maharashtra, India

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Pages:: 591–597

Online Publication Date:: 01 Dec 2010

Publication Date:: 01 Dec 2010

Article Category:: Research Article

DOI:: https://doi.org/10.1556/achrom.22.2010.4.8

Keywords:: HPLC; negundoside; PDA detector

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Summary

An HPLC method has been developed for routine analysis of negundoside in Vitex negundo Linn. leaf extract. Separation was performed on a C₁₈ column with 65:35 (v/v) buffer solution (containing 0.01 M potassium dihydrogen orthophosphate and 0.01 M heptane sulphonic acid sodium salt; pH 3.0)-methanol as mobile phase. Detection and quantification were achieved by use of UV and photo diode array detectors operated at 251 nm. Response was a linear function of negundoside concentration in the range 1–37.5 μg mL⁻¹ (correlation coefficient 0.9996). The method was validated for system and method precision, accuracy, linearity, and solution stability. The method is sensitive, simple, rapid, accurate, and precise and can therefore be used for routine qualitycontrol analysis of Vitex negundo Linn. leaf powder, including quantitative analysis of negundoside.

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Acta Chromatographica

Online ISSN:: 2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

Danica Agbaba, University of Belgrade, Belgrade, Serbia
Łukasz Komsta, Medical University of Lublin, Lublin, Poland
Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
Jacek Bojarski, Jagiellonian University, Kraków, Poland
Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
Michał Daszykowski, University of Silesia, Katowice, Poland
Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
Attila Felinger, University of Pécs, Pécs, Hungary
Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
Urszula Hubicka, Jagiellonian University, Kraków, Poland
Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
Huba Kalász, Semmelweis University, Budapest, Hungary
Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
Imre Klebovich, Semmelweis University, Budapest, Hungary
Angelika Koch, Private Pharmacy, Hamburg, Germany
Piotr Kus, Univerity of Silesia, Katowice, Poland
Debby Mangelings, Free University of Brussels, Brussels, Belgium
Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
Gertrud Morlock, Giessen University, Giessen, Germany
Anna Petruczynik, Medical University of Lublin, Lublin, Poland
Robert Skibiński, Medical University of Lublin, Lublin, Poland
Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
Adam Voelkel, Poznań University of Technology, Poznań, Poland
Beata Walczak, University of Silesia, Katowice, Poland
Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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2023
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Journal Impact Factor	1.7
Rank by Impact Factor	Q3 (Chemistry, Analytical)
Journal Citation Indicator	0.43
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CiteScore	4.0
CiteScore rank	Q2 (General Chemistry)
SNIP	0.706
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SJR index	0.344
SJR Q rank	Q3

Acta Chromatographica
Publication Model	Online only Gold Open Access
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Article Processing Charge	400 EUR/article Effective from 1st Feb 2025: 700 EUR/article
Regional discounts on country of the funding agency	World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100%
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Acta Chromatographica
Language	English
Size	A4
Year of Foundation	1988
Volumes per Year	1
Issues per Year	4
Founder	Institute of Chemistry, University of Silesia
Founder's Address	PL-40-007 Katowice, Poland, Bankowa 12
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	2083-5736 (Online)