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P. K. Gupta
P. K. Gupta
R&D, Tulip Lab Pvt. Ltd., MIDC Ranjangaon, Pune, 412220, India
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V. V. Kuber
V. V. Kuber
R&D, Tulip Lab Pvt. Ltd., MIDC Ranjangaon, Pune, 412220, India
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V. K. Ghosh
V. K. Ghosh
R&D, Tulip Lab Pvt. Ltd., MIDC Ranjangaon, Pune, 412220, India
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S. G. Bhope
S. G. Bhope
R&D, Tulip Lab Pvt. Ltd., MIDC Ranjangaon, Pune, 412220, India
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V. Sharma
V. Sharma
R&D, Tulip Lab Pvt. Ltd., MIDC Ranjangaon, Pune, 412220, India
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S. Purohit
S. Purohit
Marwar Pharmacy College, Kharda Bhandu, Main Barmer Road, Jodhpur, India
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Summary
A simple, rapid, and specific thin layer chromatographic (TLC) method has been developed and validated for the simultaneous estimation of icariin and l-arginine from commercial polyherbal formulations for sexual dysfunction. The separation of the methanol extract of these formulations was achieved on silica gel 60 F254 aluminum backed TLC plates by using ethyl acetate-acetone-glacial acetic acid-formic acid-water 12:2:1:2:2 (υ/υ) as mobile phase. Densitometric analysis of icariin and l-arginine was monitored in absorbance mode at 270 and 195 nm, respectively. The linear regression analysis data for the calibration plots for icariin and l-arginine showed good linear relationship with r2 = 0.9984 +- 0.01 and 0.9968 +- 0.02, in the concentration ranges of 250–750 and 500–1500 ng/spot, respectively. The method was validated for precision, robustness, and recovery. The average percentage recovery was found to be 98.26% for icariin and 99.63% for l-arginine. The limits of detection and quantitation were 72, 116 and 238, 383 ng/spot, respectively, for icariin and l-arginine. Statistical analysis proves that the method is repeatable and selective for the estimation of the targeted drugs. Since the proposed mobile phase effectively resolves the icariin and l-arginine, this method can be applied for the identification and quantitation of these components in herbal extracts and marketed formulations.
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[1]. M.N. Makarova O.N. Pozharitskaya A.N. Shikov S.S.V. Tesakova V.G. Makarov V.P. Tikhonov 2007 J. Ethnopharmacol. 114 412–416.
-
[2]. A.M. Dell G.V. Galli C.E. Dal F. Belluti R. Matera E. Zironi G. Paglliuca E. Bosisio 2008 J. Nat. Prod. 71 1513–1517.
-
[3]. H. Ning Z.C. Xin G. Lin L. Banie T.F. Lue C.S. Lin 2006 Urology 68 1350–1354.
-
[4]. H.B. Xu Z.Q. Huang 2007 Vascul. Pharmacol. 47 18–24.
-
[5]. Z.B. Zhang Q.Q.T. Yang 2006 Asian J. Androl. 8 601–605.
-
[6]. L. Ding X.G. Liang D. Zhu Y. Lou 2007 Acta. Pharmacol. Sin. 28 1541–1549.
-
[7]. W. He H. Sun B. Yang D. Zhang D. Kabelitz 1995 Arzneimittelforschung 45 910–913.
-
[8]. J. Liu H. Ye Y. Lou 2005 Pharmazie 60 120–125.
-
[9]. S. Moncada E.A. Higgs H.F. Hodson R.G. Knowles P. Lopez-Jaramillo T. McCall R.M.J. Palmer M.W. Radomski D.D. Rees R. Schulz 1991 J. Cardiovasc. Pharmacol. 17 Suppl.3 1–9.
-
[10]. R. Stanislavov V.J. Nikolova 2003 Sex Marital Ther. 29 207–213.
-
[11]. R.M.J. Palmer D.S. Ashton S. Moncada 1988 Nature 333 664–666.
-
[12]. O.N. Pozharitskaya V.M. Kosman A.N. Shikov D.V. Demchenko A.Y. Eschenko V.G. Makarov 2007 J. Sep. Sci. 30 708–712.
-
[13]. J.J. Liu S.P. Li Y.T. Wang 2006 J. Chromatogr. A 1103 344–349.
-
[14]. X.J. Chen B.L. Guo S.P. Li Q.W. Zhang P.F. Tu Y.T. Wang 2007 J. Chromatogr. A 1163 96–104.
-
[15]. X.J. Chen H. Ji Q.W. Zhang P.F. Tu Y.T. Wang B.L. Guo S.P. Li 2008 J. Pharm. Biomed. Anal. 46 226–235.
-
[16]. M. Liu H. Liu X. Lu C. Li Z. Xiong F. Li 2007 J. Chromatogr. B 860 113–120.
-
[17]. B. Hess J. Sherma 2004 Acta Chromatogr. 14 60–68.
-
[18]. R. Zakrzewski W. Ciesielski D. Kazmierczak 2003 J. Sep. Sci. 26 1063–1066.
-
[19]. R.N. Zare J.P. Cooke T. Chou M.T. Dulay K. Sydow G. Trapp 2004 J. Sep. Sci. 27 1483–1490.
-
[20]. V. Gopalakrishnan P.J. Burton T.F. Blaschke 1996 Anal. Chem. 68 3520–3523.
-
[21]. ICH Q2B, Harmonized tripartite guideline, validation of analytical procedures: Methodology, IFPMA. In: Proceeding of the International Conference on Harmonization (ICH), Geneva, (1996).
Senior editors
Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland
Editors(s)
- Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
- Łukasz Komsta, Medical University of Lublin, Lublin, Poland
- Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
- Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland
Editorial Board
- Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
- Jacek Bojarski, Jagiellonian University, Kraków, Poland
- Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
- Michał Daszykowski, University of Silesia, Katowice, Poland
- Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
- Attila Felinger, University of Pécs, Pécs, Hungary
- Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
- Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
- Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
- Urszula Hubicka, Jagiellonian University, Kraków, Poland
- Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
- Huba Kalász, Semmelweis University, Budapest, Hungary
- Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
- Imre Klebovich, Semmelweis University, Budapest, Hungary
- Angelika Koch, Private Pharmacy, Hamburg, Germany
- Piotr Kus, Univerity of Silesia, Katowice, Poland
- Debby Mangelings, Free University of Brussels, Brussels, Belgium
- Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
- Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
- Gertrud Morlock, Giessen University, Giessen, Germany
- Anna Petruczynik, Medical University of Lublin, Lublin, Poland
- Robert Skibiński, Medical University of Lublin, Lublin, Poland
- Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
- Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
- Adam Voelkel, Poznań University of Technology, Poznań, Poland
- Beata Walczak, University of Silesia, Katowice, Poland
- Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
- Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation
SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl
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| 2024 | |
|---|---|
| Scopus | |
| CiteScore | |
| CiteScore rank | |
| SNIP | |
| Scimago | |
| SJR index | 0.285 |
| SJR Q rank | Q3 |
| 2023 | |
|---|---|
| Web of Science | |
| Journal Impact Factor | 1.7 |
| Rank by Impact Factor | Q3 (Chemistry, Analytical) |
| Journal Citation Indicator | 0.43 |
| Scopus | |
| CiteScore | 4.0 |
| CiteScore rank | Q2 (General Chemistry) |
| SNIP | 0.706 |
| Scimago | |
| SJR index | 0.344 |
| SJR Q rank | Q3 |
| Acta Chromatographica | |
|---|---|
| Publication Model | Online only Gold Open Access |
| Submission Fee | none |
| Article Processing Charge | 700 EUR/article |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription Information | Gold Open Access |
| Purchase per Title | |
| Acta Chromatographica | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Institute of Chemistry, University of Silesia |
| Founder's Address |
PL-40-007 Katowice, Poland, Bankowa 12 |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 2083-5736 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Nov 2024 | 0 | 112 | 2 |
| Dec 2024 | 0 | 98 | 2 |
| Jan 2025 | 0 | 56 | 2 |
| Feb 2025 | 0 | 52 | 2 |
| Mar 2025 | 0 | 97 | 7 |
| Apr 2025 | 0 | 24 | 2 |
| May 2025 | 0 | 0 | 0 |
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