Acta Chromatographica
Volume/Issue:
Volume 24: Issue 1
Optimum separation condition of five bioactive compounds by HCI program in HPLC
Authors:
M. Tian Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon, 402-751, Korea

Search for other papers by M. Tian in
Current site
Google Scholar
PubMed Close
,
S. Li Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon, 402-751, Korea

Search for other papers by S. Li in
Current site
Google Scholar
PubMed Close
, and
K. H. Row Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon, 402-751, Korea

Search for other papers by K. H. Row in
Current site
Google Scholar
PubMed Close
Pages:
65–74
Online Publication Date:
01 Mar 2012
Publication Date:
01 Mar 2012
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.24.2012.1.6
Keywords:
HCI program; HPLC; separation; bioactive compounds; marine plant
Open access

Summary

An efficient method used to separate five bioactive compounds from Gelidium amansii was optimized by the HCI software. The optimum composition of mobile phase for high-performance liquid chromatographic (HPLC) separation was obtained. The elution profiles were calculated by the polynomial theory based on the retention factor ln k = A + BF + CF2 (F was the volume percentage of acetonitrile with 1.0% acetic acid); then, the theory was applied to calculate the elution profile in both isocratic and gradient modes by modifying different mobile phase conditions with HCI program. The calculated results of mobile phase condition suggested that acetonitrile-water (containing 1.0% acetic acid) with a linear gradient elution of 0∼30 min from 15:85 to 50:50 (v/v) was the optimized component. In the experimental conditions, the agreement between the experimental elution profiles and the calculated values of eluted concentration was relatively good.

  • [1]. K.H.M. Cardozo T. Guaratini M.P. Barros V.R. Falcao A.P. Tonon N.P. Lopes S. Campos M.A. Torres A.O. Souza P. Colepicolo E. Pinto 2007 Comp. Biochem. Physiol. C: Toxicol. Pharmacol. 146 60.

    • Search Google Scholar
    • Export Citation

  • [2]. N.T. Eriksen 2008 Appl. Microbiol. Biotech. 80 1.

  • [3]. M. Plaza A. Cifuentes E. Ibanez 2008 Trends Food Sci. Technol. 19 31.

  • [4]. M. Gantar Z. Svircev 2008 J. Phycol. 44 2 260.

  • [5]. F. Bettazzi I. Palchetti S. Sisalli M. Mascini 2006 Anal. Chim. Acta 555 1 134.

  • [6]. H.B. Li K.W. Cheng C.C. Wong K.W. Fan F. Chen Y. Jiang 2007 Food Chem. 102 771.

  • [7]. P. Ganesan C.S. Kumar N. Bhaskar 2008 Bioresour. Technol. 99 2717.

  • [8]. A.K. Sinha S.C. Verma U.K. Sharma 2007 J. Sep. Sci. 30 15.

  • [9]. E. Cavoy M.F. Deltent S. Lehoucq D. Miggiano 1997 J. Chromatogr. A 769 49.

  • [10]. J.H. Kennedy M.D. Belvo V.S. Sharp J.D. Williams 2004 J. Chromatogr. A 1046 55.

  • [11]. X.J. Yao X.Y. Zhang R.S. Zhang M.C. Liu Z.D. Hu B.T. Fan 2002 Talanta 57 297.

  • [12]. L. Jin D.Y. Choi Y. Wang W.S. Ahn D.K. Choi K.H. Row 2005 J. Ind. Eng. Chem. 11 4 615.

  • [13]. D.H. Kang J.W. Lee K.H. Row 2001 J. Liq. Chrom. Relat. Technol. 24 6 855.

  • [14]. C.H. Jin J.W. Lee K.H. Row 2008 J. Sep. Sci. 31 23.

  • [15]. R. Kaliszan P. Wiczling M.J. Markuszewski 2004 J. Chromatogr. A 1060 165.

  • [16]. J. Zheng K.H. Row 2007 Chin. J. Chem. Eng. 15 2 291.

  • [17]. Y. Jin K.H. Row 2006 Chin. J. Chromatogr. 24 5 466.

  • [18]. S.V. Galushko A.A. Kamenchuk G.L. Pit 1994 J. Chromatogr. A 660 47.

  • [19]. L.R. Snyder J.W. Dolan 1998 Adv. Chromatogr. 38 115.

  • [20]. L.R. Snyder J.W. Dolan J.R. Gant 1979 J. Chromatogr. 165 3.

  • [21]. W. Markowski W. Golkiewicz 1988 Chromatographia 25 339.

  • [22]. P.J. Schoenmakers H.A.H. Billiet L. Galan 1979 J. Chromatogr. A 185 179.

  • Collapse
  • Expand
Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • SCOPUS
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2024 0 15 5
May 2024 0 10 9
Jun 2024 0 23 3
Jul 2024 0 7 1
Aug 2024 0 33 4
Sep 2024 0 18 2
Oct 2024 0 5 1