Authors:
Y. Chen Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine, Southern Medical University, 510515, Guangzhou, People's Republic of China

Search for other papers by Y. Chen in
Current site
Google Scholar
PubMed
Close
,
X. Li Chifeng Aike Pharmaceutical Technology Co., Ltd., 024001, Chifeng, People's Republic of China

Search for other papers by X. Li in
Current site
Google Scholar
PubMed
Close
,
F. Chen Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine, Southern Medical University, 510515, Guangzhou, People's Republic of China

Search for other papers by F. Chen in
Current site
Google Scholar
PubMed
Close
,
Q. Zhu Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine, Southern Medical University, 510515, Guangzhou, People's Republic of China

Search for other papers by Q. Zhu in
Current site
Google Scholar
PubMed
Close
, and
J. Luo Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine, Southern Medical University, 510515, Guangzhou, People's Republic of China

Search for other papers by J. Luo in
Current site
Google Scholar
PubMed
Close
Open access

Summary

A rapid, simple, and practical high-performance liquid chromatographic method (HPLC) was developed and validated for the simultaneous determination of norephedrine (NME), norpseudoephedrine (NMP), ephedrine (E), pseudoephedrine (PE), and methylephedrine (ME) in traditional Chinese medicines (TCM) which contained Ephedrae Herba (Ephedra). This analysis could be accomplished within 12.5 min with an Alltima Phenyl Column by isocratic elution using a mixture of KH2PO4 (20 mM)-acetonitrile (96:4, v/v) as the mobile phase at a flow-rate of 0.6 mL min−1 and a wavelength of 210 nm. This method was successfully applied to quantify ephedra alkaloids in both Ma-xing-gan-shi decoction and Ephedra decoction. The concentration of total ephedra alkaloids (4.62 mg mL−1) in Ma-xing-gan-shi decoction was much lower than that (7.10 mg mL−1) in Ephedra decoction. Furthermore, the concentration of NME, NMP, E, PE, and ME was significantly lower in Ma-xing-gan-shi decoction than that in Ephedra decoction, respectively. The method was easily acceptable and would be popular with most analytical laboratories.

  • [1]. F.J. Duan 1999 Formula of Traditional Chinese Medicine China Press of Traditional Chinese Medicine Beijing 8083.

  • [2]. E.A. Abourashed A.T. El-Alfy I.A. Khan L. Walker 2003 Phytother. Res. 17 703712.

  • [3]. W.H. Organization 1999 Herba Ephedrae WHO Monographs on Selected Medicinal Plants Publications of the World Health Organization Geneva 145163.

    • Search Google Scholar
    • Export Citation
  • [4]. S.M. Wang R.J. Lewis D. Canfield T.L. Li C.Y. Chen R.H. Liu 2005 J. Chromatogr. B. 825 8895.

  • [5]. P.R. Garcia M. Yonamine 2005 Moreau RLDM Revista Brasileira de Ciencias Farmaceuticas 41 351358.

  • [6]. E. Marchei M. Pellegrini R. Pacifici P. Zuccaro S. Pichini 2006 J. Pharmaceut. Biomed. 41 16331641.

  • [7]. J.F. Cui T.H. Zhou J.S. Zhang Z.C. Lou 1991 Phytochem. Anal. 2 116119.

  • [8]. K.H. Chan R.N. Pan M.C. Hsu 2005 Biomed. Chromatogr. 19 337342.

  • [9]. N. Okamura H. Miki T. Harada S. Yamashita Y. Masaoka Y. Nakamoto M. Tsuguma H. Yoshitomi A. Yagi 1999 J. Pharm. Biomed. Anal. 20 363372.

    • Search Google Scholar
    • Export Citation
  • [10]. B.J. Gurley P. Wang S.F. Gardner 1998 J. Pharm. Sci. 87 15471553.

  • [11]. C. Imaz D. Carreras R. Navajas C. Rodriguez A.F. Rodriguez J. Maynar R. Cortes 1993 J. Chromatogr. A 631 201205.

  • [12]. M.C. Roman 2004 J. AOAC Int. 87 114.

  • [13]. J.A. Hurlbut J.R. Carr E.R. Singleton K.C. Faul M.R. Madson J.M. Storey T.L. Thomas 1998 J. AOAC Int. 81 11211127.

  • [14]. M. Ganzera C. Lanser H. Stuppner 2005 Talanta 66 889894.

  • [15]. S.J. Sheu M.H. Huang 2001 Chromatographia 54 117119.

  • [16]. B.T. Schaneberg I.A. Khan 2004 Pharmazie 59 819823.

  • [17]. K.W. Phinney T. Ihara L.C. Sander 2005 J. Chromatogr. A 1077 9097.

  • [18]. N. Miyatake H. Miyake M. Nagashima M. Takahashi K. Yasuda I. Yasuda 2004 Yakugaku Zasshi 124 333339.

  • [19]. C.L. Flurer L.A. Lin R.D. Satzger K.A. Wolnik 1995 J. Chromatogr. B: Biomed. Appl. 669 133139.

  • [20]. B. Wiedemer and C. Thrall, LC GC N. Am., S, 43 (2004).

  • [21]. K. Sagara T. Oshima T. Misaki 1983 Chem. Pharm. Bull. 31 23592365.

  • [22]. Q.H. Zhu F.L. Chen S. Bai J.B. Luo 2005 Chin. J. Pharm. Anal. 25 10301034.

  • [23]. F. Pellati S. Benvenuti 2008 J. Pharmaceut. Biomed. 48 254263.

  • [24]. J. Li 1992 “Shang Han Lun” Fang Zheng Yao Yan Jiu Heilongjiang Science and Technology Press Haerbin 73.

  • [25]. Q. Wang 1988 “Shang Han Lun” Jiang Jie Henan Science and Technology Press Zhengzhou 82.

  • [26]. W.J. Lin K. Chen L. Lin 2004 Chin. J. Pharm. Anal. 24 378380.

  • [27]. C. Imaz R. Navajas D. Carreras C. Rodriguez A.F. Rodriguez 2000 J. Chromatogr. A 870 2328.

  • [28]. J.S. Zhang Z. Tian Z.C. Lou 1989 Yao Xue Xue Bao 24 865871.

  • Collapse
  • Expand
The author instructions are available in PDF.
Please, download the file from HERE.

 

 

Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • SCOPUS
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2024 0 102 8
Mar 2024 0 40 8
Apr 2024 0 21 14
May 2024 0 5 6
Jun 2024 0 30 9
Jul 2024 0 15 5
Aug 2024 0 0 0