This study reports the effect of a nonionic perfluorinated surfactant, N-polyoxyethylene-N-propyl perfluorooctane sulfonamide (PFOSA), as additive of background electrolyte on capillary electrophoresis (CE) of common inorganic cations. The association constants (Kass) for PFOSA estimated from the electrophoretic mobility of analyte cations were the order of Mg2+ > Ca2+ > Sr2+ > K+ ≈ NH4+ > Na+ ≈ Li+. The Kass values were larger than those for zwitterionic and nonionic surfactants with hydrocarbon moiety. Use of PFOSA made another essential contribution to the determination of inorganic cations in a protein-containing sample. This was considered because high solubility of PFOSA for proteins functioned as suppressor for protein adsorption to the capillary wall. Four inorganic cations, Na+, K+, Mg2+, and Ca2+, in human saliva sample were successfully determined by sample injection without any pretreatments except for filtration and dilution.
. N.A.Guzman1993Capillary Electrophoresis Technology in Chromatography Science SeriesMarcel DekkerNew York.
N.A.Guzman1993Capillary Electrophoresis Technology in Chromatography Science SeriesMarcel DekkerNew York.)| false