Summary
A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid-liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.
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[1]. JEF. Reynolds. Martindale, The Extra Pharmacopoeia, The Royal Pharmaceutical Society, Great Britain, London; 2006, pp. 594–596.
-
[2]. E.D. Ralf , Clin. Pharm., 8 (1983).
-
[3]. J.G. Hardman L.E. Limbird A.G. Gilman 2001 Goodman & Gilman's. The pharmacological basis of therapeutics 10th ed. Mc Graw-Hill NewYork.
-
[4]. C. Gjerloff , E. Arnold, Acta. Pharmacol. Toxicol, 51 (1982).
-
[5]. B. Horatio , T. Thien, Clin. Ther., 27 (2005).
-
[6]. A.O. Adegoke , O.K.E. Umoh, J.O. Soyinka. J. Chem. Soc., 7 (2010).
-
[7]. E. Vega , N. Sola. J. Pharm. Biomed. Anal., 25 (2001).
-
[8]. British Pharmacopoeia, Her Majestry Stationery Office, London, 2002, pp. 98, 1814 and 1815.
-
[9]. A. Ozkan , Y. Ozkan, Z. Senturk, J. Pharm. Biomed. Anal., 17 (1998).
-
[10]. W. Jin , W. Li, Q. Xu, Q. Dong, Electrophoresis, 21 (2000).
-
[11]. M.J. Jessa , D.A. Barrett, P.N. Shaw, R.C. Spiller, J. Chromatogr. B, 677 (1996).
-
[12]. J. Emami , N. Ghassami, H. Hamishehkar, Daru, 14 (2006).
-
[13]. M.J. Galmier , A.M. Frasey, M. Bastide, E. Beyssac, J. Petit, J.M. Aiache, C. Lartigue-Mattei, J. Chromatogr. B, 720 (1998).
-
[14]. R.A. Gibson , L. Lattanzio, H. Mcgee, Clin. Chem., 30 (1984).
-
[15]. H.W. Sun , F.C. Wang, L.F. Aie, J.Chromatogr. B. 857 (2007).
-
[16]. T.J. Venkateswaran , J.T. Stewart, J. Chromatogr. B, 672 (1995).
-
[17]. K.B. Mustapha , M.T. Odunola, M. Garba, O. Obodozie. J. Biotech., 5 (2006).
-
[18]. Y-H. Tu , Y. Wang, L. Allen, Int. J. Pharmaceutic., 61 (1990).
-
[19]. M. Silva , S. Schramm, E. Kanoe Kono, V. Port A. C. Serra. J. Chromatogr. Sci., 47 (2009).
-
[20]. P. Chaikin , K.B. Alton, D.C. Sampson, W. Weintraub, J. Clin. Pharmaco., 22 (1982).
-
[21]. M. Bakshi , S. Singh, J. Pharmaceutic. Biomed. Anal., 34 (2004).
-
[22]. K. Phasa , A. Ali, S. Bana, and S. Humair, Int. J. Pharm. Pharmaceutic. SIC, 2 (2010).
-
[23]. K. Rajnarayana , M.R. Chaluvadi, V.R. Alapati, S.R. Mada, G. Jayasagar, D.R. Krishna, Pharmazie, 57 (2002).
-
[24]. I. Nilson-Ehle , B.O. Ursing, P. Nilson-Ehle, J. Antimicrob. Chemo., 19 (1981).
-
[25]. J Mattlla , T. Manntsto, R. Mantyla, S. Nykanen, Antimicrog. Agent. Chemother., 23 (1983).
-
[26]. J. Virtanen , H. Haataja, P.T Mannisto, J. Antimicrob. Agent. Chemother., 28 (1985).
-
[27]. H. Mattie , B A.C. Dijkmans, C. Gulpen, J. Antimicrob. Chemother., 1 (1982).
-
[28]. A.L Crowell , A Kolby, W.E Secor, Antimicrob. Agent. Chemother., 47 (2003).
-
[29]. L. Shargel S. W. Pong A.B.C. Yu 2005 Applied biopharmaceutics and pharmacokinetics 5th ed. MC Graw Hill New York 435–475.
-
[30]. A. Menelaou , A.A Somogyi, M.L Barclay, F. Bochner, J. Chromatogr. B, 27 (1999).
Senior editors
Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)
Editor(s)-in-Chief: Sajewicz, Mieczyslaw
Editors(s)
- Danica Agbaba (University of Belgrade, Belgrade, Serbia)
- Łukasz Komsta (Medical University of Lublin, Lublin, Poland)
- Ivana Stanimirova-Daszykowska (University of Silesia, Katowice, Poland)
- Monika Waksmundzka-Hajnos (Medical University of Lublin, Lublin, Poland)
Editorial Board
- R. Bhushan (The Indian Institute of Technology, Roorkee, India)
- J. Bojarski (Jagiellonian University, Kraków, Poland)
- B. Chankvetadze (State University of Tbilisi, Tbilisi, Georgia)
- M. Daszykowski (University of Silesia, Katowice, Poland)
- T.H. Dzido (Medical University of Lublin, Lublin, Poland)
- A. Felinger (University of Pécs, Pécs, Hungary)
- K. Glowniak (Medical University of Lublin, Lublin, Poland)
- B. Glód (Siedlce University of Natural Sciences and Humanities, Siedlce, Poland)
- A. Gumieniczek (Medical University of Lublin, Lublin, Poland)
- U. Hubicka (Jagiellonian University, Kraków, Poland)
- K. Kaczmarski (Rzeszow University of Technology, Rzeszów, Poland)
- H. Kalász (Semmelweis University, Budapest, Hungary)
- K. Karljiković Rajić (University of Belgrade, Belgrade, Serbia)
- I. Klebovich (Semmelweis University, Budapest, Hungary)
- A. Koch (Private Pharmacy, Hamburg, Germany)
- P. Kus (Univerity of Silesia, Katowice, Poland)
- D. Mangelings (Free University of Brussels, Brussels, Belgium)
- E. Mincsovics (Corvinus University of Budapest, Budapest, Hungary)
- Á. M. Móricz (Centre for Agricultural Research, Budapest, Hungary)
- G. Morlock (Giessen University, Giessen, Germany)
- A. Petruczynik (Medical University of Lublin, Lublin, Poland)
- R. Skibiński (Medical University of Lublin, Lublin, Poland)
- B. Spangenberg (Offenburg University of Applied Sciences, Germany)
- T. Tuzimski (Medical University of Lublin, Lublin, Poland)
- Y. Vander Heyden (Free University of Brussels, Brussels, Belgium)
- A. Voelkel (Poznań University of Technology, Poznań, Poland)
- B. Walczak (University of Silesia, Katowice, Poland)
- W. Wasiak (Adam Mickiewicz University, Poznań, Poland)
- I.G. Zenkevich (St. Petersburg State University, St. Petersburg, Russian Federation)
KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl
SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl
Indexing and Abstracting Services:
- Science Citation Index
- Sci Search
- Research Alert
- Chemistry Citation Index and Current Content/Physical
- Chemical and Earth Sciences
- SCOPUS
- GoogleScholar
- Index Copernicus
- CABI
- CABELLS Journalytics
| 2021 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
652 |
| Journal Impact Factor | 2,011 |
| Rank by Impact Factor | Chemistry, Analytical 66/87 |
| Impact Factor without Journal Self Cites |
1,789 |
| 5 Year Impact Factor |
1,350 |
| Journal Citation Indicator | 0,40 |
| Rank by Journal Citation Indicator | Chemistry, Analytical 72/99 |
| Scimago | |
| Scimago H-index |
29 |
| Scimago Journal Rank |
0,27 |
| Scimago Quartile Score | Chemistry (miscellaneous) (Q3) |
| Scopus | |
| Scopus Cite Score |
2,8 |
| Scopus CIte Score Rank |
General Chemistry 210/409 (Q3) |
| Scopus SNIP |
0,586 |
|
2020
|
|
|
Total Cites
|
650
|
|
WoS
|
|
|
Journal
Impact Factor |
1,639
|
|
Rank by
|
Chemistry, Analytical 71/83 (Q4)
|
|
Impact Factor
|
|
|
Impact Factor
|
1,412
|
|
without
|
|
|
Journal Self Cites
|
|
|
5 Year
|
1,301
|
|
Impact Factor
|
|
|
Journal
|
0,34
|
|
Citation Indicator
|
|
|
Rank by Journal
|
Chemistry, Analytical 75/93 (Q4)
|
|
Citation Indicator
|
|
|
Citable
|
45
|
|
Items
|
|
|
Total
|
43
|
|
Articles
|
|
|
Total
|
2
|
|
Reviews
|
|
|
Scimago
|
28
|
|
H-index
|
|
|
Scimago
|
0,316
|
|
Journal Rank
|
|
|
Scimago
|
Chemistry (miscellaneous) Q3
|
|
Quartile Score
|
|
|
Scopus
|
393/181=2,2
|
|
Scite Score
|
|
|
Scopus
|
General Chemistry 215/398 (Q3)
|
|
Scite Score Rank
|
|
|
Scopus
|
0,560
|
|
SNIP
|
|
|
Days from
|
58
|
|
submission
|
|
|
to acceptance
|
|
|
Days from
|
68
|
|
acceptance
|
|
|
to publication
|
|
|
Acceptance
|
51%
|
| Rate |
| 2019 | |
|---|---|
| Total Cites WoS |
495 |
| Impact Factor | 1,418 |
| Impact Factor without Journal Self Cites |
1,374 |
| 5 Year Impact Factor |
0,936 |
| Immediacy Index |
0,460 |
| Citable Items |
50 |
| Total Articles |
50 |
| Total Reviews |
0 |
| Cited Half-Life |
6,2 |
| Citing Half-Life |
8,3 |
| Eigenfactor Score |
0,00048 |
| Article Influence Score |
0,164 |
| % Articles in Citable Items |
100,00 |
| Normalized Eigenfactor |
0,05895 |
| Average IF Percentile |
20,349 |
| Scimago H-index |
26 |
| Scimago Journal Rank |
0,255 |
| Scopus Scite Score |
226/167=1,4 |
| Scopus Scite Score Rank |
Chemistry (miscellaneous) 240/398 (Q3) |
| Scopus SNIP |
0,494 |
| Acceptance Rate |
41% |
| Acta Chromatographica | |
|---|---|
| Publication Model | Online only Gold Open Access |
| Submission Fee | none |
| Article Processing Charge | 400 EUR/article |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription Information | Gold Open Access |
| Purchase per Title | |
| Acta Chromatographica | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Institute of Chemistry, University of Silesia |
| Founder's Address |
PL-40-007 Katowice, Poland, Bankowa 12 |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 2083-5736 (Online) |
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| Abstract Views | Full Text Views | PDF Downloads | |
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| Dec 2022 | 0 | 19 | 14 |
| Jan 2023 | 0 | 22 | 8 |
| Feb 2023 | 0 | 4 | 5 |
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| Apr 2023 | 0 | 5 | 10 |
| May 2023 | 0 | 6 | 7 |
| Jun 2023 | 0 | 0 | 0 |
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