Acta Chromatographica
Volume/Issue:
Volume 25: Issue 2
Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry
Authors:
H. L. Huo Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by H. L. Huo in
Current site
Google Scholar
PubMed Close
,
S. H. Yu Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by S. H. Yu in
Current site
Google Scholar
PubMed Close
,
X. Z. Liu Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by X. Z. Liu in
Current site
Google Scholar
PubMed Close
,
Y. Meng Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by Y. Meng in
Current site
Google Scholar
PubMed Close
,
Y. P. Ren Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by Y. P. Ren in
Current site
Google Scholar
PubMed Close
, and
L. T. Zhang Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China

Search for other papers by L. T. Zhang in
Current site
Google Scholar
PubMed Close
Pages:
201–219
Online Publication Date:
01 Jun 2013
Publication Date:
01 Jun 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.25.2013.2.1
Keywords:
HPLC-MS/MS; excretion; coumarins
Open access

Summary

A selective and sensitive liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was developed and validated for analysis of xanthotoxol (1), xanthotoxin (2), isoimpinellin (3), bergapten (4), oxypeucedanin (5), imperatorin (6), cnidilin (7), and isoimperatorin (8) in rat bile and urine using pimpinellin as an internal standard (IS). An Agilent 1200 liquid chromatography system (Agilent Technologies, USA) equipped with a quaternary pump, an autosampler, and a column compartment was used for all analyses. Chromatographic separations were performed on a Sapphire C18 column (150 mm × 4.6 mm, 5 μm), and the column temperature was maintained at 30°C; the sample injection volume was 10 μL. The specificity, linearity, accuracy, precision, recovery, matrix effect, and several stabilities were validated for all analytes in the rat bile and urine samples. The method was successfully applied in monitoring the concentrations of eight coumarins in rat bile and urine after a single oral administration of Radix Angelicae Dahuricae extract with a dosage of 8.0 mL/kg. In the bile samples, the eight coumarins excreted completely in twenty-four hours. The average percentages of coumarins (1–8) excreted were 0.045%, 0.019%, 0.177%, 0.105%, 0.337%, 0.023%, 0.024%, 0.021%. In the urine samples, the eight coumarins excreted completely in seventy-two hours. The average percentages of coumarins (1–8) excreted were 1.78%, 0.095%, 0.130%, 0.292%, 0.082%, 0.008%, 0.005%, 0.004%. The method is robust and specific and it can successfully complete the requirements of the excretion study of the eight coumarins in Radix Angelicae Dahuricae.

  • [1]. H. Zhu D.Z. Kong H.J. Xu N. Wang C.C. Zhao P.W. Liu L.T. Zhang 2011 Asian, J. Chem. 23 3893–3897.

  • [2]. T.T. Wang H. Jin Q. Li W.M. Cheng Q.Q. Hu X.H. Chen K.S. Bi 2007 Chromatographia 65 477–481.

  • [3]. Y. Xie W.Q. Zhao T.T. Zhou G.R. Fan Y.T. Wu 2010 Phytochem. Anal. 21 473–482.

  • [4]. L.P. Xiang I.H. Park S.H. Back H.Y. Kim M.K. Park J.H. Park 2004 J. Ethnopharmacol. 93 243–246.

  • [5]. Y. Kimura H. Ohminami H. Arichi H. Okuda K. Baba M. Kozawa S. Arichi 1997 J. Nat. Prod. 60 249–251.

  • [6]. F. Cottiglia G. Loy D. Garau C. Floris M. Casu R. Pompei L. Bonsignore 2001 Phytomedicine 8 302–305.

  • [7]. Y.S. Kwon A. Kobayashi S. Kajiyama K. Kawazu H. Kanzaki C.M. Kim 1997 Phytochemistry 44 887–889.

  • [8]. S.X. Deng Y.Q. Mo 1988 Chin. Trad. Herbal Drugs 19 22–25.

  • [9]. B.S. Adama R. Pentza C.P. Siegersa O. Strubelt M. Tegtmeier 2005 Phytomedicine 12 52–61.

  • [10]. J.Y. Deng G.H. Gao C.J. Zhao P.J. Xu 2004 J. Shenyang Pharmaceut. Univ. 21 354–357.

  • [11]. A.Y. Park S.Y. Park J. Lee M. Jung J. Kim S.S. Kang J.R. Youm S.B. Han 2009 Biomed. Chromatogr. 23 1034–1043.

  • [12]. X.G. Zheng X.W. Zhang X.N. Sheng Z.F. Yuan W. Yang Q. Wang L.T. Zhang 2010 J. Pharmaceut. Biomed. Anal. 51 599–605.

  • [13]. W. Yang C. Feng D.Z. Kong X.W. Shi X.G. Zheng Y. Cui M. Liu L.T. Zhang Q. Wang 2010 Food Chem. 120 886–894.

  • [14]. Y.B. Li 2006 J. Pharmaceut. Biomed. Anal. 40 1253–1256.

  • [15]. Y. Xie Y. Chen M. Lin J. Wen G.R. Fan Y.T. Wu 2007 J. Pharmaceut. Biomed. Anal. 44 166–172.

  • [16]. S.C. Wang Q.H. Chen L.C. He 2007 J. Chromatogr. B 852 473–478.

  • [17]. T.F. Jing Z.H. Lv Y.H. Wang M.E. Yue 2010 Biomed. Chromatogr. 24 581–587.

  • [18]. W. Yang C. Feng D.Z. Kong X.W. Shi Y. Cui M. Liu Q. Wang Y.L. Wang L.T. Zhang 2010 J. Chromatogr. B 878 575–582.

  • [19]. Y.F. Du P.W. Liu X.W. Shi Y.R. Jin Q. Wang X.W. Zhang X.N. Sheng L.T. Zhang 2010 Anal. Biochem. 407 111–119.

  • [20]. H. Zhu L.T. Zhang Y.P. Ren H.J. Xu Y. Cui 2011 Chin. J. Pharmaceut. 5 112–115.

  • Collapse
  • Expand
Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • SCOPUS
  • DOAJ
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2024  
Scopus  
CiteScore  
CiteScore rank  
SNIP  
Scimago  
SJR index 0.285
SJR Q rank Q3

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge

700 EUR/article

For MS submitted before 1 Feb 2025: 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2024 0 106 7
Jan 2025 0 49 6
Feb 2025 0 77 3
Mar 2025 0 108 7
Apr 2025 0 35 9
May 2025 0 21 6
Jun 2025 0 32 2